Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace
In this study, a novel polysaccharide, LPP, was obtained from <i>Lonicera caerulea</i> L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 × 10<sup>4</sup> Da; that i...
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MDPI AG
2022-08-01
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| Series: | Fermentation |
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| author | Fangyi Pei Yuze Lv Xinbo Cao Xuemeng Wang Yanxin Ren Jingping Ge |
| author_facet | Fangyi Pei Yuze Lv Xinbo Cao Xuemeng Wang Yanxin Ren Jingping Ge |
| author_sort | Fangyi Pei |
| collection | DOAJ |
| description | In this study, a novel polysaccharide, LPP, was obtained from <i>Lonicera caerulea</i> L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 × 10<sup>4</sup> Da; that it was mainly composed of galacturonic acid, followed by galactose; that it possessed the characteristic functional groups of polysaccharides; and that it had an absence of O-glycosidic bonds and crystalline and triple helix structures. Furthermore, LPP exhibited a favorable thermodynamic stability and antioxidant, hypoglycemic, and hypolipidemic activities in a dose-dependent manner in vitro, demonstrating that LPP can be used as an agent to regulate glycolipid metabolism. Additionally, the relationship between its bio-activities is discussed in this paper. The results revealed that the RP, •OH, and NO<sub>2</sub><sup>−</sup> radicals had synergistic promoting effects, and polysaccharides with a strong antioxidant ability may have excellent hypoglycemic and hypolipidemic effects. Collectively, these results suggest that LPP has a strong bio-activity, and that <i>Lonicera caerulea</i> L. pomace can be used as a potential polysaccharide source. |
| first_indexed | 2024-03-10T00:04:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T00:04:04Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj.art-2cf12aaba734446b894d6362db337d582023-11-23T16:10:26ZengMDPI AGFermentation2311-56372022-08-018942210.3390/fermentation8090422Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. PomaceFangyi Pei0Yuze Lv1Xinbo Cao2Xuemeng Wang3Yanxin Ren4Jingping Ge5Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaKey Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaKey Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaKey Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaKey Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaKey Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, ChinaIn this study, a novel polysaccharide, LPP, was obtained from <i>Lonicera caerulea</i> L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 × 10<sup>4</sup> Da; that it was mainly composed of galacturonic acid, followed by galactose; that it possessed the characteristic functional groups of polysaccharides; and that it had an absence of O-glycosidic bonds and crystalline and triple helix structures. Furthermore, LPP exhibited a favorable thermodynamic stability and antioxidant, hypoglycemic, and hypolipidemic activities in a dose-dependent manner in vitro, demonstrating that LPP can be used as an agent to regulate glycolipid metabolism. Additionally, the relationship between its bio-activities is discussed in this paper. The results revealed that the RP, •OH, and NO<sub>2</sub><sup>−</sup> radicals had synergistic promoting effects, and polysaccharides with a strong antioxidant ability may have excellent hypoglycemic and hypolipidemic effects. Collectively, these results suggest that LPP has a strong bio-activity, and that <i>Lonicera caerulea</i> L. pomace can be used as a potential polysaccharide source.https://www.mdpi.com/2311-5637/8/9/422blue honeysucklepolysaccharidestructural characterizationsglycolipid metabolismcorrelation analysis |
| spellingShingle | Fangyi Pei Yuze Lv Xinbo Cao Xuemeng Wang Yanxin Ren Jingping Ge Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace Fermentation blue honeysuckle polysaccharide structural characterizations glycolipid metabolism correlation analysis |
| title | Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace |
| title_full | Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace |
| title_fullStr | Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace |
| title_full_unstemmed | Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace |
| title_short | Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace |
| title_sort | structural characteristics and the antioxidant and hypoglycemic activities of a polysaccharide from i lonicera caerulea i l pomace |
| topic | blue honeysuckle polysaccharide structural characterizations glycolipid metabolism correlation analysis |
| url | https://www.mdpi.com/2311-5637/8/9/422 |
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