Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo
Objective The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2022-11-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-22-0040.pdf |
| _version_ | 1811233036777291776 |
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| author | Soyoung Choi Jihye Cha Minji Song JuHwan Son Mi-Rim Park Yeong-jo Lim Tae-Hun Kim Kyung-Tai Lee Woncheoul Park |
| author_facet | Soyoung Choi Jihye Cha Minji Song JuHwan Son Mi-Rim Park Yeong-jo Lim Tae-Hun Kim Kyung-Tai Lee Woncheoul Park |
| author_sort | Soyoung Choi |
| collection | DOAJ |
| description | Objective The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship. |
| first_indexed | 2024-04-12T11:14:19Z |
| format | Article |
| id | doaj.art-2cf2cb93669a4ac186cf68a4366e6a42 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-12T11:14:19Z |
| publishDate | 2022-11-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-2cf2cb93669a4ac186cf68a4366e6a422022-12-22T03:35:33ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352022-11-0135111808181610.5713/ab.22.004024931Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in HanwooSoyoung Choi0Jihye Cha1Minji Song2JuHwan Son3Mi-Rim Park4Yeong-jo Lim5Tae-Hun Kim6Kyung-Tai Lee7Woncheoul Park8 Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, Korea Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju 55365, KoreaObjective The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship.http://www.animbiosci.org/upload/pdf/ab-22-0040.pdffeceshanwoomicrobiome16s rrnavagina |
| spellingShingle | Soyoung Choi Jihye Cha Minji Song JuHwan Son Mi-Rim Park Yeong-jo Lim Tae-Hun Kim Kyung-Tai Lee Woncheoul Park Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo Animal Bioscience feces hanwoo microbiome 16s rrna vagina |
| title | Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo |
| title_full | Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo |
| title_fullStr | Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo |
| title_full_unstemmed | Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo |
| title_short | Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo |
| title_sort | analysis of 16s rrna gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in hanwoo |
| topic | feces hanwoo microbiome 16s rrna vagina |
| url | http://www.animbiosci.org/upload/pdf/ab-22-0040.pdf |
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