An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles
Abstract The emerging antibiotic resistance in pathogenic bacteria is a key problem in modern medicine that has led to a search for novel therapeutic strategies. A potential approach for managing such bacteria involves the use of their natural killers, namely lytic bacteriophages. Another effective...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-59866-y |
| _version_ | 1797199529078947840 |
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| author | Mateusz Szymczak Jarosław A. Pankowski Agnieszka Kwiatek Bartłomiej Grygorcewicz Joanna Karczewska-Golec Kamila Sadowska Piotr Golec |
| author_facet | Mateusz Szymczak Jarosław A. Pankowski Agnieszka Kwiatek Bartłomiej Grygorcewicz Joanna Karczewska-Golec Kamila Sadowska Piotr Golec |
| author_sort | Mateusz Szymczak |
| collection | DOAJ |
| description | Abstract The emerging antibiotic resistance in pathogenic bacteria is a key problem in modern medicine that has led to a search for novel therapeutic strategies. A potential approach for managing such bacteria involves the use of their natural killers, namely lytic bacteriophages. Another effective method involves the use of metal nanoparticles with antimicrobial properties. However, the use of lytic phages armed with nanoparticles as an effective antimicrobial strategy, particularly with respect to biofilms, remains unexplored. Here, we show that T7 phages armed with silver nanoparticles exhibit greater efficacy in terms of controlling bacterial biofilm, compared with phages or nanoparticles alone. We initially identified a novel silver nanoparticle-binding peptide, then constructed T7 phages that successfully displayed the peptide on the outer surface of the viral head. These recombinant, AgNP-binding phages could effectively eradicate bacterial biofilm, even when used at low concentrations. Additionally, when used at concentrations that could eradicate bacterial biofilm, T7 phages armed with silver nanoparticles were not toxic to eukaryotic cells. Our results show that the novel combination of lytic phages with phage-bound silver nanoparticles is an effective, synergistic and safe strategy for the treatment of bacterial biofilms. |
| first_indexed | 2024-04-24T07:17:12Z |
| format | Article |
| id | doaj.art-2cf2de658cf345f995ba88f218b7d322 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:17:12Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2cf2de658cf345f995ba88f218b7d3222024-04-21T11:15:24ZengNature PortfolioScientific Reports2045-23222024-04-0114111510.1038/s41598-024-59866-yAn effective antibiofilm strategy based on bacteriophages armed with silver nanoparticlesMateusz Szymczak0Jarosław A. Pankowski1Agnieszka Kwiatek2Bartłomiej Grygorcewicz3Joanna Karczewska-Golec4Kamila Sadowska5Piotr Golec6Department of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawDepartment of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawDepartment of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawDepartment of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawDepartment of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawNalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of SciencesDepartment of Molecular Virology, Faculty of Biology, Institute of Microbiology, University of WarsawAbstract The emerging antibiotic resistance in pathogenic bacteria is a key problem in modern medicine that has led to a search for novel therapeutic strategies. A potential approach for managing such bacteria involves the use of their natural killers, namely lytic bacteriophages. Another effective method involves the use of metal nanoparticles with antimicrobial properties. However, the use of lytic phages armed with nanoparticles as an effective antimicrobial strategy, particularly with respect to biofilms, remains unexplored. Here, we show that T7 phages armed with silver nanoparticles exhibit greater efficacy in terms of controlling bacterial biofilm, compared with phages or nanoparticles alone. We initially identified a novel silver nanoparticle-binding peptide, then constructed T7 phages that successfully displayed the peptide on the outer surface of the viral head. These recombinant, AgNP-binding phages could effectively eradicate bacterial biofilm, even when used at low concentrations. Additionally, when used at concentrations that could eradicate bacterial biofilm, T7 phages armed with silver nanoparticles were not toxic to eukaryotic cells. Our results show that the novel combination of lytic phages with phage-bound silver nanoparticles is an effective, synergistic and safe strategy for the treatment of bacterial biofilms.https://doi.org/10.1038/s41598-024-59866-y |
| spellingShingle | Mateusz Szymczak Jarosław A. Pankowski Agnieszka Kwiatek Bartłomiej Grygorcewicz Joanna Karczewska-Golec Kamila Sadowska Piotr Golec An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles Scientific Reports |
| title | An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| title_full | An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| title_fullStr | An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| title_full_unstemmed | An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| title_short | An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| title_sort | effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles |
| url | https://doi.org/10.1038/s41598-024-59866-y |
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