Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies
In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and B...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Finlay Ediciones
2010-04-01
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| Series: | VacciMonitor |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2010000100004&lng=es&nrm=iso |
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| author | Le Thuy Nguyen Thi Reinier Borrero Maura Sonsire Férnandez Giselle Reyes José Luis Perez Fátima Reyes María de los Angeles García Midrey Fariñas Juan Francisco Infante Yanely Tirado Alina Puig Gustavo Sierra Nadine Álvarez Juan Carlos Ramírez María Elena Sarmiento Mohd-Nor Norazmi Armando Acosta |
| author_facet | Le Thuy Nguyen Thi Reinier Borrero Maura Sonsire Férnandez Giselle Reyes José Luis Perez Fátima Reyes María de los Angeles García Midrey Fariñas Juan Francisco Infante Yanely Tirado Alina Puig Gustavo Sierra Nadine Álvarez Juan Carlos Ramírez María Elena Sarmiento Mohd-Nor Norazmi Armando Acosta |
| author_sort | Le Thuy Nguyen Thi |
| collection | DOAJ |
| description | In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies |
| first_indexed | 2024-12-22T15:56:24Z |
| format | Article |
| id | doaj.art-2cf44e356c0d4703be0b33c7095b10c1 |
| institution | Directory Open Access Journal |
| issn | 1025-028X 1025-0298 |
| language | English |
| last_indexed | 2024-12-22T15:56:24Z |
| publishDate | 2010-04-01 |
| publisher | Finlay Ediciones |
| record_format | Article |
| series | VacciMonitor |
| spelling | doaj.art-2cf44e356c0d4703be0b33c7095b10c12022-12-21T18:20:46ZengFinlay EdicionesVacciMonitor1025-028X1025-02982010-04-011912026Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studiesLe Thuy Nguyen Thi0Reinier Borrero Maura1Sonsire Férnandez2Giselle Reyes3José Luis Perez4Fátima Reyes5María de los Angeles García6Midrey Fariñas7Juan Francisco Infante8Yanely Tirado9Alina Puig10Gustavo Sierra11Nadine Álvarez12Juan Carlos Ramírez13María Elena Sarmiento14Mohd-Nor Norazmi15Armando Acosta16Biotechnology Centre of Ho Chi Minh City, 176 Hai Ba Trung Street, Ho Chi Minh City, VietnamFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaSchool of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, MalaysiaFinlay Institute. Center for Vaccine Research & Production. Ave 27 No 19805, La Lisa, Havana City, AP16017 CP11600, CubaIn this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategieshttp://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2010000100004&lng=es&nrm=isoMycobacterium tuberculosisMycobacterium smegmatisepitopesprediction |
| spellingShingle | Le Thuy Nguyen Thi Reinier Borrero Maura Sonsire Férnandez Giselle Reyes José Luis Perez Fátima Reyes María de los Angeles García Midrey Fariñas Juan Francisco Infante Yanely Tirado Alina Puig Gustavo Sierra Nadine Álvarez Juan Carlos Ramírez María Elena Sarmiento Mohd-Nor Norazmi Armando Acosta Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies VacciMonitor Mycobacterium tuberculosis Mycobacterium smegmatis epitopes prediction |
| title | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_full | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_fullStr | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_full_unstemmed | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_short | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_sort | evaluation of the potential of mycobacterium smegmatis as vaccine candidate against tuberculosis by in silico and in vivo studies |
| topic | Mycobacterium tuberculosis Mycobacterium smegmatis epitopes prediction |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2010000100004&lng=es&nrm=iso |
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