Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology
Secondary metabolites extract of McB1 endophytic fungi from gelam (Melaleuca cajuputi Powell.) leaves have a high potential antibacterial activity against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 with flavonoids and phenol as bioactive compound. The low production of secondary me...
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| Format: | Article |
| Language: | English |
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Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya
2022-04-01
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| Series: | Science and Technology Indonesia |
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| Online Access: | https://sciencetechindonesia.com/index.php/jsti/article/view/440 |
| _version_ | 1797285784980553728 |
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| author | Hary Widjajanti Elisa Nurnawati Muharni Eca Desriana Zahwa |
| author_facet | Hary Widjajanti Elisa Nurnawati Muharni Eca Desriana Zahwa |
| author_sort | Hary Widjajanti |
| collection | DOAJ |
| description | Secondary metabolites extract of McB1 endophytic fungi from gelam (Melaleuca cajuputi Powell.) leaves have a high potential antibacterial activity against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 with flavonoids and phenol as bioactive compound. The low production of secondary metabolites extract in the cultivation stage and the high potential antibacterial activity of bioactive compounds produced by McB1 endophytic fungi require special treatment for optimize the secondary metabolites product. This is possibly achieved by optimizing the composition of the cultivation media, where various sources of carbon, nitrogen, and pH produce different amounts and classes of secondary metabolites. The objectives of the research to obtain the optimum interaction between sources of carbon, nitrogen, and pH for the production of secondary metabolite extract using Response Surface Methodology (RSM). The results showed that the highest extract (0.25 g) with the composition of sucrose as carbon source, yeast extract as nitrogen source, and pH 6. Based on the optimization of the medium with a variation of 4.5 gL-1 sucrose, 0.48 gL-1 yeast extract, and pH 6.1 yielded 0.34 g of secondary metabolites extract of McB1 endophytic fungi. The chromatogram profile of the optimized secondary metabolite extract showed the presence of flavonoids, phenols, terpenoids, and tannins. |
| first_indexed | 2024-03-07T18:08:21Z |
| format | Article |
| id | doaj.art-2cf99547f16e413d95ee480689a57062 |
| institution | Directory Open Access Journal |
| issn | 2580-4405 2580-4391 |
| language | English |
| last_indexed | 2024-03-07T18:08:21Z |
| publishDate | 2022-04-01 |
| publisher | Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya |
| record_format | Article |
| series | Science and Technology Indonesia |
| spelling | doaj.art-2cf99547f16e413d95ee480689a570622024-03-02T08:06:02ZengMagister Program of Material Sciences, Graduate School of Universitas SriwijayaScience and Technology Indonesia2580-44052580-43912022-04-017214915710.26554/sti.2022.7.2.149-157Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface MethodologyHary Widjajanti 0Elisa Nurnawati 1Muharni 2Eca Desriana Zahwa 3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Palembang, 30662, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Palembang, 30662, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Palembang, 30662, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Palembang, 30662, IndonesiaSecondary metabolites extract of McB1 endophytic fungi from gelam (Melaleuca cajuputi Powell.) leaves have a high potential antibacterial activity against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 with flavonoids and phenol as bioactive compound. The low production of secondary metabolites extract in the cultivation stage and the high potential antibacterial activity of bioactive compounds produced by McB1 endophytic fungi require special treatment for optimize the secondary metabolites product. This is possibly achieved by optimizing the composition of the cultivation media, where various sources of carbon, nitrogen, and pH produce different amounts and classes of secondary metabolites. The objectives of the research to obtain the optimum interaction between sources of carbon, nitrogen, and pH for the production of secondary metabolite extract using Response Surface Methodology (RSM). The results showed that the highest extract (0.25 g) with the composition of sucrose as carbon source, yeast extract as nitrogen source, and pH 6. Based on the optimization of the medium with a variation of 4.5 gL-1 sucrose, 0.48 gL-1 yeast extract, and pH 6.1 yielded 0.34 g of secondary metabolites extract of McB1 endophytic fungi. The chromatogram profile of the optimized secondary metabolite extract showed the presence of flavonoids, phenols, terpenoids, and tannins.https://sciencetechindonesia.com/index.php/jsti/article/view/440endophytic fungiantibacterial compoundresponse surface methodology (rsm) |
| spellingShingle | Hary Widjajanti Elisa Nurnawati Muharni Eca Desriana Zahwa Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology Science and Technology Indonesia endophytic fungi antibacterial compound response surface methodology (rsm) |
| title | Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology |
| title_full | Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology |
| title_fullStr | Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology |
| title_full_unstemmed | Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology |
| title_short | Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology |
| title_sort | optimization of antibacterial production of endophytic fungi with various sources of c n and ph using the response surface methodology |
| topic | endophytic fungi antibacterial compound response surface methodology (rsm) |
| url | https://sciencetechindonesia.com/index.php/jsti/article/view/440 |
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