Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace
Raspberry fruit pomace, a byproduct of juice production, was studied as a potential source of antioxidant compounds. Target high-performance liquid chromatography analysis of important polyphenolic compounds (gallic, <i>p</i>-coumaric, caffeic, quercitrin, chlorogenic, and ellagic acid)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/4/706 |
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| author | Slađana Krivokapić Milorad Vlaović Biljana Damjanović Vratnica Andrej Perović Svetlana Perović |
| author_facet | Slađana Krivokapić Milorad Vlaović Biljana Damjanović Vratnica Andrej Perović Svetlana Perović |
| author_sort | Slađana Krivokapić |
| collection | DOAJ |
| description | Raspberry fruit pomace, a byproduct of juice production, was studied as a potential source of antioxidant compounds. Target high-performance liquid chromatography analysis of important polyphenolic compounds (gallic, <i>p</i>-coumaric, caffeic, quercitrin, chlorogenic, and ellagic acid) was performed together with analysis of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and antioxidant capacity (via ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays). The differences in polyphenolic content of <i>Rubus idaeus</i> L. pomace were evaluated following ultrasound-assisted extraction and conventional maceration with different organic solvents. Additionally, the yield of free phenolics was measured in hydrolyzed pomace extracts. The results obtained show that the ultrasound method maximizes the quantity of antioxidant compounds in terms of TPC (27.79 mg/L gallic acid equivalent (GAE)), TFC (8.02 mg/g quercetin equivalent (QE)), TAC (7.13 mg/L cyanidine-3-glucoside equivalent (C3G Eq)), caffeic (19.17 µg/g), chlorogenic (3.56 µg/g), ellagic (105.52 µg/g), and gallic acids (8.75 µg/g), as well as FRAP (1002.72 µmol/L) and DPPH assays (969.71 µmol/mL vitamin C equivalent (vit C Eq); 567.00 µmol/100 g Trolox equivalent (TE)). On the other hand, conventional maceration maximizes the yield of quercetin and <i>p</i>-coumaric acid. In terms of biowaste valorization, raspberry fruit pomace has a promising industrial potential and may prove to be useful in the development of antioxidant dietary supplements. |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T12:52:04Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2cfaac0abbac433dae2c4574352b8e5d2023-11-21T12:10:39ZengMDPI AGFoods2304-81582021-03-0110470610.3390/foods10040706Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit PomaceSlađana Krivokapić0Milorad Vlaović1Biljana Damjanović Vratnica2Andrej Perović3Svetlana Perović4Department of Biology, Faculty of Natural Science and Mathematics, University of Montenegro, Podgorica 81000, MontenegroDepartment of Biology, Faculty of Natural Science and Mathematics, University of Montenegro, Podgorica 81000, MontenegroFaculty of Metallurgy and Technology, University of Montenegro, Podgorica 81000, MontenegroDepartment of Biology, Faculty of Natural Science and Mathematics, University of Montenegro, Podgorica 81000, MontenegroDepartment of Biology, Faculty of Natural Science and Mathematics, University of Montenegro, Podgorica 81000, MontenegroRaspberry fruit pomace, a byproduct of juice production, was studied as a potential source of antioxidant compounds. Target high-performance liquid chromatography analysis of important polyphenolic compounds (gallic, <i>p</i>-coumaric, caffeic, quercitrin, chlorogenic, and ellagic acid) was performed together with analysis of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and antioxidant capacity (via ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays). The differences in polyphenolic content of <i>Rubus idaeus</i> L. pomace were evaluated following ultrasound-assisted extraction and conventional maceration with different organic solvents. Additionally, the yield of free phenolics was measured in hydrolyzed pomace extracts. The results obtained show that the ultrasound method maximizes the quantity of antioxidant compounds in terms of TPC (27.79 mg/L gallic acid equivalent (GAE)), TFC (8.02 mg/g quercetin equivalent (QE)), TAC (7.13 mg/L cyanidine-3-glucoside equivalent (C3G Eq)), caffeic (19.17 µg/g), chlorogenic (3.56 µg/g), ellagic (105.52 µg/g), and gallic acids (8.75 µg/g), as well as FRAP (1002.72 µmol/L) and DPPH assays (969.71 µmol/mL vitamin C equivalent (vit C Eq); 567.00 µmol/100 g Trolox equivalent (TE)). On the other hand, conventional maceration maximizes the yield of quercetin and <i>p</i>-coumaric acid. In terms of biowaste valorization, raspberry fruit pomace has a promising industrial potential and may prove to be useful in the development of antioxidant dietary supplements.https://www.mdpi.com/2304-8158/10/4/706antioxidantspolyphenolsHPLCbyproduct |
| spellingShingle | Slađana Krivokapić Milorad Vlaović Biljana Damjanović Vratnica Andrej Perović Svetlana Perović Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace Foods antioxidants polyphenols HPLC byproduct |
| title | Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace |
| title_full | Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace |
| title_fullStr | Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace |
| title_full_unstemmed | Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace |
| title_short | Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace |
| title_sort | biowaste as a potential source of bioactive compounds a case study of raspberry fruit pomace |
| topic | antioxidants polyphenols HPLC byproduct |
| url | https://www.mdpi.com/2304-8158/10/4/706 |
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