Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
Maize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a diffe...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.693088/full |
| _version_ | 1819098557725016064 |
|---|---|
| author | Huyen Bui Robert Greenhalgh Gunbharpur S. Gill Meiyuan Ji Andre H. Kurlovs Christian Ronnow Sarah Lee Ricardo A. Ramirez Richard M. Clark Richard M. Clark |
| author_facet | Huyen Bui Robert Greenhalgh Gunbharpur S. Gill Meiyuan Ji Andre H. Kurlovs Christian Ronnow Sarah Lee Ricardo A. Ramirez Richard M. Clark Richard M. Clark |
| author_sort | Huyen Bui |
| collection | DOAJ |
| description | Maize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a different mechanism. Two spider mites, the generalist Tetranychus urticae, and the grass-specialist Oligonychus pratensis, are notable pests of maize, especially during drought conditions. We assessed resistance (antibiosis) to both mites of 38 highly diverse maize lines, including several previously reported to be resistant to one or the other mite species. We found that line B96, as well as its derivatives B49 and B75, were highly resistant to T. urticae. In contrast, neither these three lines, nor any others included in our study, were notably resistant to the specialist O. pratensis. Quantitative trait locus (QTL) mapping with replicate populations from crosses of B49, B75, and B96 to susceptible B73 identified a QTL in the same genomic interval on chromosome 6 for T. urticae resistance in each of the three resistant lines, and an additional resistance QTL on chromosome 1 was unique to B96. Single-locus genotyping with a marker coincident with the chromosome 6 QTL in crosses of both B49 and B75 to B73 revealed that the respective QTL was large-effect; it explained ∼70% of the variance in resistance, and resistance alleles from B49 and B75 acted recessively as compared to B73. Finally, a genome-wide haplotype analysis using genome sequence data generated for B49, B75, and B96 identified an identical haplotype, likely of initial origin from B96, as the source of T. urticae resistance on chromosome 6 in each of the B49, B75, and B96 lines. Our findings uncover the relationship between intraspecific variation in maize defenses and resistance to its major generalist and specialist spider mite herbivores, and we identified loci for use in breeding programs and for genetic studies of resistance to T. urticae, the most widespread spider mite pest of maize. |
| first_indexed | 2024-12-22T00:32:53Z |
| format | Article |
| id | doaj.art-2cfb538be7ad47d5b6df459e12471dc9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T00:32:53Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2cfb538be7ad47d5b6df459e12471dc92022-12-21T18:44:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.693088693088Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider MitesHuyen Bui0Robert Greenhalgh1Gunbharpur S. Gill2Meiyuan Ji3Andre H. Kurlovs4Christian Ronnow5Sarah Lee6Ricardo A. Ramirez7Richard M. Clark8Richard M. Clark9School of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesDepartment of Biology, Utah State University, Logan, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesDepartment of Biology, Utah State University, Logan, UT, United StatesSchool of Biological Sciences, University of Utah, Salt Lake City, UT, United StatesHenry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT, United StatesMaize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a different mechanism. Two spider mites, the generalist Tetranychus urticae, and the grass-specialist Oligonychus pratensis, are notable pests of maize, especially during drought conditions. We assessed resistance (antibiosis) to both mites of 38 highly diverse maize lines, including several previously reported to be resistant to one or the other mite species. We found that line B96, as well as its derivatives B49 and B75, were highly resistant to T. urticae. In contrast, neither these three lines, nor any others included in our study, were notably resistant to the specialist O. pratensis. Quantitative trait locus (QTL) mapping with replicate populations from crosses of B49, B75, and B96 to susceptible B73 identified a QTL in the same genomic interval on chromosome 6 for T. urticae resistance in each of the three resistant lines, and an additional resistance QTL on chromosome 1 was unique to B96. Single-locus genotyping with a marker coincident with the chromosome 6 QTL in crosses of both B49 and B75 to B73 revealed that the respective QTL was large-effect; it explained ∼70% of the variance in resistance, and resistance alleles from B49 and B75 acted recessively as compared to B73. Finally, a genome-wide haplotype analysis using genome sequence data generated for B49, B75, and B96 identified an identical haplotype, likely of initial origin from B96, as the source of T. urticae resistance on chromosome 6 in each of the B49, B75, and B96 lines. Our findings uncover the relationship between intraspecific variation in maize defenses and resistance to its major generalist and specialist spider mite herbivores, and we identified loci for use in breeding programs and for genetic studies of resistance to T. urticae, the most widespread spider mite pest of maize.https://www.frontiersin.org/articles/10.3389/fpls.2021.693088/fulltwo-spotted spider mite (Tetranychus urticae)Tetranychus cinnabarinusBanks grass mitebulked segregant analysis (BSA)antibiosisOligonychus pratensis |
| spellingShingle | Huyen Bui Robert Greenhalgh Gunbharpur S. Gill Meiyuan Ji Andre H. Kurlovs Christian Ronnow Sarah Lee Ricardo A. Ramirez Richard M. Clark Richard M. Clark Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites Frontiers in Plant Science two-spotted spider mite (Tetranychus urticae) Tetranychus cinnabarinus Banks grass mite bulked segregant analysis (BSA) antibiosis Oligonychus pratensis |
| title | Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites |
| title_full | Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites |
| title_fullStr | Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites |
| title_full_unstemmed | Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites |
| title_short | Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites |
| title_sort | maize inbred line b96 is the source of large effect loci for resistance to generalist but not specialist spider mites |
| topic | two-spotted spider mite (Tetranychus urticae) Tetranychus cinnabarinus Banks grass mite bulked segregant analysis (BSA) antibiosis Oligonychus pratensis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.693088/full |
| work_keys_str_mv | AT huyenbui maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT robertgreenhalgh maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT gunbharpursgill maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT meiyuanji maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT andrehkurlovs maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT christianronnow maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT sarahlee maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT ricardoaramirez maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT richardmclark maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites AT richardmclark maizeinbredlineb96isthesourceoflargeeffectlociforresistancetogeneralistbutnotspecialistspidermites |