Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay
Background: Despite the wide range of applications, there is a serious lack of information on the impact of the nanoparticles on human health and the environment. The present study was done to determine the range of dangerous concentrations of iron oxide nanoparticle and their effects on mouse embry...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2016-07-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=216&sid=1&slc_lang=en |
| _version_ | 1819024448256212992 |
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| author | Homa Mohseni Kouchesfehani Sahar Kiani Ali Akbar Rostami Rahman Fakheri |
| author_facet | Homa Mohseni Kouchesfehani Sahar Kiani Ali Akbar Rostami Rahman Fakheri |
| author_sort | Homa Mohseni Kouchesfehani |
| collection | DOAJ |
| description | Background: Despite the wide range of applications, there is a serious lack of information on the impact of the nanoparticles on human health and the environment. The present study was done to determine the range of dangerous concentrations of iron oxide nanoparticle and their effects on mouse embryonic stem cells.
Methods: Iron oxide nanoparticles with less than 20 nanometers diameter were encapsulated by a PEG-phospholipid. The suspension of iron oxide nanoparticles was prepared using the culture media and cell viability was determined by MTT assay.
Results: MTT assay was used to examine the cytotoxicity of iron oxide nanoparticle s. Royan B1 cells were treated with medium containing different concentrations (10, 20, 30, 40, 50, and 60µg/ml) of the iron oxide nanoparticle. Cell viability was determined at 12 and 24 hours after treatment which showed significant decreases when concentration and time period increased.
Conclusion: The main mechanism of nanoparticles action is still unknown, but in vivo and in vitro studies in different environments suggest that they are capable of producing reactive oxygen species (ROS). Therefore, they may have an effect on the concentration of intracellular calcium, activation of transcription factors, and changes in cytokine. The results of this study show that the higher concentration and duration of treatment of cells with iron oxide nanoparticles increase the rate of cell death. |
| first_indexed | 2024-12-21T04:54:57Z |
| format | Article |
| id | doaj.art-2cffc401672d4783b9d15290252b5341 |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-21T04:54:57Z |
| publishDate | 2016-07-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-2cffc401672d4783b9d15290252b53412022-12-21T19:15:22ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592016-07-01721849853Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT AssayHoma Mohseni Kouchesfehani0Sahar Kiani1Ali Akbar Rostami2Rahman Fakheri3Department of Biological Sciences, Kharazmi University, Tehran, Iran.Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.MSc student in Developmental Biology Sciences, Kharazmi University, Tehran, Iran.*Corresponding Author: E-mail: aliakbarrostami6616@yahoo.comBSc in Biology, Reproductive Biomedicine Research Center, Royan Institute for Animal Biotechnology, ACECR, Tehran, Iran.Background: Despite the wide range of applications, there is a serious lack of information on the impact of the nanoparticles on human health and the environment. The present study was done to determine the range of dangerous concentrations of iron oxide nanoparticle and their effects on mouse embryonic stem cells. Methods: Iron oxide nanoparticles with less than 20 nanometers diameter were encapsulated by a PEG-phospholipid. The suspension of iron oxide nanoparticles was prepared using the culture media and cell viability was determined by MTT assay. Results: MTT assay was used to examine the cytotoxicity of iron oxide nanoparticle s. Royan B1 cells were treated with medium containing different concentrations (10, 20, 30, 40, 50, and 60µg/ml) of the iron oxide nanoparticle. Cell viability was determined at 12 and 24 hours after treatment which showed significant decreases when concentration and time period increased. Conclusion: The main mechanism of nanoparticles action is still unknown, but in vivo and in vitro studies in different environments suggest that they are capable of producing reactive oxygen species (ROS). Therefore, they may have an effect on the concentration of intracellular calcium, activation of transcription factors, and changes in cytokine. The results of this study show that the higher concentration and duration of treatment of cells with iron oxide nanoparticles increase the rate of cell death.http://ijt.arakmu.ac.ir/browse.php?a_id=216&sid=1&slc_lang=enCytotoxicityIron Oxide NanoparticleMTT Assay |
| spellingShingle | Homa Mohseni Kouchesfehani Sahar Kiani Ali Akbar Rostami Rahman Fakheri Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay Iranian Journal of Toxicology Cytotoxicity Iron Oxide Nanoparticle MTT Assay |
| title | Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay |
| title_full | Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay |
| title_fullStr | Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay |
| title_full_unstemmed | Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay |
| title_short | Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay |
| title_sort | cytotoxic effect of iron oxide nanoparticles on mouse embryonic stem cells by mtt assay |
| topic | Cytotoxicity Iron Oxide Nanoparticle MTT Assay |
| url | http://ijt.arakmu.ac.ir/browse.php?a_id=216&sid=1&slc_lang=en |
| work_keys_str_mv | AT homamohsenikouchesfehani cytotoxiceffectofironoxidenanoparticlesonmouseembryonicstemcellsbymttassay AT saharkiani cytotoxiceffectofironoxidenanoparticlesonmouseembryonicstemcellsbymttassay AT aliakbarrostami cytotoxiceffectofironoxidenanoparticlesonmouseembryonicstemcellsbymttassay AT rahmanfakheri cytotoxiceffectofironoxidenanoparticlesonmouseembryonicstemcellsbymttassay |