Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology
The outbreak of novel coronavirus pneumonia (COVID-19), defined as a worldwide pandemic, has been a public health emergency of international concern. Pudilanxiaoyan oral liquid (PDL), an effective drug of Traditional Chinese Medicine (TCM), is considered to be an effective and alternative means for...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Georg Thieme Verlag KG
2021-06-01
|
| Series: | Pharmaceutical Fronts |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1735147 |
| _version_ | 1819019744868564992 |
|---|---|
| author | Ying-Peng Tong Xiao-Fei Shen Chao Li Qi Zhou Chun-Xiao Jiang Na Li Zhen-Da Xie Zi-Ping Zhu Jian-Xin Wang |
| author_facet | Ying-Peng Tong Xiao-Fei Shen Chao Li Qi Zhou Chun-Xiao Jiang Na Li Zhen-Da Xie Zi-Ping Zhu Jian-Xin Wang |
| author_sort | Ying-Peng Tong |
| collection | DOAJ |
| description | The outbreak of novel coronavirus pneumonia (COVID-19), defined as a worldwide pandemic, has been a public health emergency of international concern. Pudilanxiaoyan oral liquid (PDL), an effective drug of Traditional Chinese Medicine (TCM), is considered to be an effective and alternative means for clinical prevention of COVID-19. The purpose of this study was to identify potential active constituents of PDL, and explore its underlying anti-COVID-19 mechanism using network pharmacology. Integration of target prediction (SwissTargetPrediction and STITCH database) was used to elucidate the active components of PDL. Protein–protein interaction network analyses, gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, network construction, and molecular docking were applied to analyze the prospective mechanisms of the predicted target genes. Our results showed that the key active ingredients in PDL were luteolin, apigenin, esculetin, chrysin, baicalein, oroxylin A, baicalin, wogonin, cymaroside, and gallic acid. A majority of the predicted targets were mainly involved in the pathways related to viral infection, lung injury, and inflammatory responses. An in vitro study further inferred that inhibiting the activity of nuclear factor (NF)-кB signaling pathway was a key mechanism by which PDL exerted anti-COVID-19 effects. This study not only provides chemical basis and pharmacology of PDL but also the rationale for strategies to exploring future TCM for COVID-19 therapy. |
| first_indexed | 2024-12-21T03:40:11Z |
| format | Article |
| id | doaj.art-2d065af806704f2cab1443b212877f00 |
| institution | Directory Open Access Journal |
| issn | 2628-5088 2628-5096 |
| language | English |
| last_indexed | 2024-12-21T03:40:11Z |
| publishDate | 2021-06-01 |
| publisher | Georg Thieme Verlag KG |
| record_format | Article |
| series | Pharmaceutical Fronts |
| spelling | doaj.art-2d065af806704f2cab1443b212877f002022-12-21T19:17:14ZengGeorg Thieme Verlag KGPharmaceutical Fronts2628-50882628-50962021-06-010302e65e7610.1055/s-0041-1735147Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network PharmacologyYing-Peng Tong0Xiao-Fei Shen1Chao Li2Qi Zhou3Chun-Xiao Jiang4Na Li5Zhen-Da Xie6Zi-Ping Zhu7Jian-Xin Wang8Institute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaTCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of ChinaJiangsu Key Laboratory of Chinese Medicine and Characteristic Preparations for Paediatrics, Jumpcan Pharmaceutical Co., Ltd., Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaInstitute of Natural Medicine and Health Product, School of Advanced Study, Taizhou University, Taizhou, People's Republic of ChinaThe outbreak of novel coronavirus pneumonia (COVID-19), defined as a worldwide pandemic, has been a public health emergency of international concern. Pudilanxiaoyan oral liquid (PDL), an effective drug of Traditional Chinese Medicine (TCM), is considered to be an effective and alternative means for clinical prevention of COVID-19. The purpose of this study was to identify potential active constituents of PDL, and explore its underlying anti-COVID-19 mechanism using network pharmacology. Integration of target prediction (SwissTargetPrediction and STITCH database) was used to elucidate the active components of PDL. Protein–protein interaction network analyses, gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, network construction, and molecular docking were applied to analyze the prospective mechanisms of the predicted target genes. Our results showed that the key active ingredients in PDL were luteolin, apigenin, esculetin, chrysin, baicalein, oroxylin A, baicalin, wogonin, cymaroside, and gallic acid. A majority of the predicted targets were mainly involved in the pathways related to viral infection, lung injury, and inflammatory responses. An in vitro study further inferred that inhibiting the activity of nuclear factor (NF)-кB signaling pathway was a key mechanism by which PDL exerted anti-COVID-19 effects. This study not only provides chemical basis and pharmacology of PDL but also the rationale for strategies to exploring future TCM for COVID-19 therapy.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1735147pudilanxiaoyan oral liquidcovid-19active ingredientsnetwork pharmacologynf-кb signaling pathway |
| spellingShingle | Ying-Peng Tong Xiao-Fei Shen Chao Li Qi Zhou Chun-Xiao Jiang Na Li Zhen-Da Xie Zi-Ping Zhu Jian-Xin Wang Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology Pharmaceutical Fronts pudilanxiaoyan oral liquid covid-19 active ingredients network pharmacology nf-кb signaling pathway |
| title | Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology |
| title_full | Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology |
| title_fullStr | Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology |
| title_full_unstemmed | Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology |
| title_short | Unveiling Potential Active Constituents and Pharmacological Mechanisms of Pudilanxiaoyan Oral Liquid for Anti-Coronavirus Pneumonia Using Network Pharmacology |
| title_sort | unveiling potential active constituents and pharmacological mechanisms of pudilanxiaoyan oral liquid for anti coronavirus pneumonia using network pharmacology |
| topic | pudilanxiaoyan oral liquid covid-19 active ingredients network pharmacology nf-кb signaling pathway |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1735147 |
| work_keys_str_mv | AT yingpengtong unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT xiaofeishen unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT chaoli unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT qizhou unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT chunxiaojiang unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT nali unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT zhendaxie unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT zipingzhu unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology AT jianxinwang unveilingpotentialactiveconstituentsandpharmacologicalmechanismsofpudilanxiaoyanoralliquidforanticoronaviruspneumoniausingnetworkpharmacology |