Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte
This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in <i>D. dasycarpus.</i> Haplopine (12.5 and 25 μM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF,...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/806 |
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| author | Tae-Young Kim Ye Jin Kim Jonghwan Jegal Beom-Geun Jo Han-Seok Choi Min Hye Yang |
| author_facet | Tae-Young Kim Ye Jin Kim Jonghwan Jegal Beom-Geun Jo Han-Seok Choi Min Hye Yang |
| author_sort | Tae-Young Kim |
| collection | DOAJ |
| description | This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in <i>D. dasycarpus.</i> Haplopine (12.5 and 25 μM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF, and G-CSF and the protein expressions of IL-6 and GM-CSF in TNF-α/INF-γ-stimulated HaCaT cells. In H<sub>2</sub>O<sub>2</sub>-induced Jukat T cells, haplopine (25 and 50 μM) suppressed the productions of proinflammatory cytokines (IL-4, IL-13, and COX-2) and increased the mRNA and protein expressions of oxidative stress defense enzymes (SOD, CAT, and HO-1) in a concentration-dependent manner. In vivo, haplopine significantly attenuated the development of AD symptoms in 2,4-dinitrochlorobenzene (DNCB)-stimulated Balb/c mice, as evidenced by reduced clinical dermatitis scores, skin thickness measurements, mast cell infiltration, and serum IgE concentrations. These findings demonstrate that haplopine should be considered a novel anti-atopic agent with the potential to treat AD. |
| first_indexed | 2024-03-10T11:14:58Z |
| format | Article |
| id | doaj.art-2d0683154b9f457ab01967ecdf60279e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:14:58Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-2d0683154b9f457ab01967ecdf60279e2023-11-21T20:28:50ZengMDPI AGAntioxidants2076-39212021-05-0110580610.3390/antiox10050806Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human KeratinocyteTae-Young Kim0Ye Jin Kim1Jonghwan Jegal2Beom-Geun Jo3Han-Seok Choi4Min Hye Yang5College of Pharmacy, Pusan National University, Busan 46241, KoreaC&D Research Team, R&D Strategy Center, Genuonesciences, Seoul 06800, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaC&D Research Team, R&D Strategy Center, Genuonesciences, Seoul 06800, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaThis study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in <i>D. dasycarpus.</i> Haplopine (12.5 and 25 μM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF, and G-CSF and the protein expressions of IL-6 and GM-CSF in TNF-α/INF-γ-stimulated HaCaT cells. In H<sub>2</sub>O<sub>2</sub>-induced Jukat T cells, haplopine (25 and 50 μM) suppressed the productions of proinflammatory cytokines (IL-4, IL-13, and COX-2) and increased the mRNA and protein expressions of oxidative stress defense enzymes (SOD, CAT, and HO-1) in a concentration-dependent manner. In vivo, haplopine significantly attenuated the development of AD symptoms in 2,4-dinitrochlorobenzene (DNCB)-stimulated Balb/c mice, as evidenced by reduced clinical dermatitis scores, skin thickness measurements, mast cell infiltration, and serum IgE concentrations. These findings demonstrate that haplopine should be considered a novel anti-atopic agent with the potential to treat AD.https://www.mdpi.com/2076-3921/10/5/806haplopineatopic dermatitisHaCaT cellsJurkat T cells2,4-dinitrochlorobenzeneBalb/c mice |
| spellingShingle | Tae-Young Kim Ye Jin Kim Jonghwan Jegal Beom-Geun Jo Han-Seok Choi Min Hye Yang Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte Antioxidants haplopine atopic dermatitis HaCaT cells Jurkat T cells 2,4-dinitrochlorobenzene Balb/c mice |
| title | Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte |
| title_full | Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte |
| title_fullStr | Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte |
| title_full_unstemmed | Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte |
| title_short | Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte |
| title_sort | haplopine ameliorates 2 4 dinitrochlorobenzene induced atopic dermatitis like skin lesions in mice and tnf α ifn γ induced inflammation in human keratinocyte |
| topic | haplopine atopic dermatitis HaCaT cells Jurkat T cells 2,4-dinitrochlorobenzene Balb/c mice |
| url | https://www.mdpi.com/2076-3921/10/5/806 |
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