Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus
Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducte...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2021-12-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1363 |
| _version_ | 1797394257413144576 |
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| author | İlyas Deligoz Miray Arlı-Sökmen Mucella Tekeoglu |
| author_facet | İlyas Deligoz Miray Arlı-Sökmen Mucella Tekeoglu |
| author_sort | İlyas Deligoz |
| collection | DOAJ |
| description |
Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducted by seven different governmental agricultural research institutes in Turkey, and advanced breeding lines have been developed by using the selected local dry bean populations and crossing studies. In this study, 204 breeding lines were tested for resistance levels to BCMV and BCMNV. Initially, BCMNV NL-3 and BCMV NL-4 strains were individually sap-inoculated onto the leaves of bean plants belonging to each breeding lines with 10 replications, and the reactions of plants were evaluated for symptomatic appearance of virus infection 21 days after inoculation. Additionally, phenotypic evaluation was confirmed by molecular markers linked to resistance genes. As a result of the study, 153 breeding lines were found to involve the dominant I gene whereas four and five of the tested lines had the recessive genes bc-1² and bc-2², respectively. In conclusion, it was emphasized that these breeding lines could be registered after evaluating them in terms of yield and quality. Also, the use of seeds of the resistant lines to supply the source of virus-resistance in breeding studies and maintaining their seeds at the national genebank were recommended.
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| first_indexed | 2024-03-09T00:17:00Z |
| format | Article |
| id | doaj.art-2d086512f05541fd87ff399cfda77349 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:17:00Z |
| publishDate | 2021-12-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-2d086512f05541fd87ff399cfda773492023-12-12T07:49:14ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052021-12-0120610.24326/asphc.2021.6.2Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virusİlyas Deligoz0Miray Arlı-Sökmen1Mucella Tekeoglu2Department of Plant Health, Black Sea Agricultural Research Institute, Samsun, TurkeyDepartment of Plant Protection, Faculty of Agriculture, Ondokuz Mayis University, Samsun, TurkeyDepartment of Plant Biotechnology, Faculty of Agriculture, Ondokuz Mayis University, Samsun, Turkey Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducted by seven different governmental agricultural research institutes in Turkey, and advanced breeding lines have been developed by using the selected local dry bean populations and crossing studies. In this study, 204 breeding lines were tested for resistance levels to BCMV and BCMNV. Initially, BCMNV NL-3 and BCMV NL-4 strains were individually sap-inoculated onto the leaves of bean plants belonging to each breeding lines with 10 replications, and the reactions of plants were evaluated for symptomatic appearance of virus infection 21 days after inoculation. Additionally, phenotypic evaluation was confirmed by molecular markers linked to resistance genes. As a result of the study, 153 breeding lines were found to involve the dominant I gene whereas four and five of the tested lines had the recessive genes bc-1² and bc-2², respectively. In conclusion, it was emphasized that these breeding lines could be registered after evaluating them in terms of yield and quality. Also, the use of seeds of the resistant lines to supply the source of virus-resistance in breeding studies and maintaining their seeds at the national genebank were recommended. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1363Phaseolus vulgarisBCMVBCMNVdominant I generecessive genes |
| spellingShingle | İlyas Deligoz Miray Arlı-Sökmen Mucella Tekeoglu Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus Acta Scientiarum Polonorum: Hortorum Cultus Phaseolus vulgaris BCMV BCMNV dominant I gene recessive genes |
| title | Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| title_full | Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| title_fullStr | Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| title_full_unstemmed | Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| title_short | Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| title_sort | phenotypic and molecular screening of dry bean phaseolus vulgaris l breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus |
| topic | Phaseolus vulgaris BCMV BCMNV dominant I gene recessive genes |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1363 |
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