Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity
Porcine epidemic diarrhea (PED) is a highly contagious disease that has been reported annually in several Asian countries, causing significant economic losses to the swine livestock industry. Although vaccines against the porcine epidemic diarrhea virus (PEDV) are available, their efficacy remains q...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/5/923 |
| _version_ | 1827739952098574336 |
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| author | Dae-Min Kim Sung-Hyun Moon Seung-Chai Kim Ho-Seong Cho Dongseob Tark |
| author_facet | Dae-Min Kim Sung-Hyun Moon Seung-Chai Kim Ho-Seong Cho Dongseob Tark |
| author_sort | Dae-Min Kim |
| collection | DOAJ |
| description | Porcine epidemic diarrhea (PED) is a highly contagious disease that has been reported annually in several Asian countries, causing significant economic losses to the swine livestock industry. Although vaccines against the porcine epidemic diarrhea virus (PEDV) are available, their efficacy remains questionable due to limitations such as viral genome mutation and insufficient intestinal mucosal immunity. Therefore, the development of a safe and effective vaccine is necessary. In this study, a virulent Korean strain of PEDV, CKT-7, was isolated from a piglet with severe diarrhea, and six different conditions were employed for serial passage of the strain in a cell culture system to generate effective live attenuated vaccine (LAV) candidates. The characteristics of these strains were analyzed in vitro and in vivo, and the CKT-7 N strain was identified as the most effective vaccine candidate, with a viral titer peak of 8.67 ± 0.29 log<sub>10</sub>TCID<sub>50</sub>/mL, and no mortality or diarrhea symptoms were observed in five-day-old piglets. These results indicate that LAV candidates can be generated through serial passage with different culture conditions and provide valuable insights into the development of a highly effective LAV against PEDV. |
| first_indexed | 2024-03-11T03:15:11Z |
| format | Article |
| id | doaj.art-2d086ded357847f4a690ab0d40455ef4 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T03:15:11Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-2d086ded357847f4a690ab0d40455ef42023-11-18T03:35:47ZengMDPI AGVaccines2076-393X2023-04-0111592310.3390/vaccines11050923Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease ActivityDae-Min Kim0Sung-Hyun Moon1Seung-Chai Kim2Ho-Seong Cho3Dongseob Tark4Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 545431, Republic of KoreaCollege of Veterinary Medicine, Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Republic of KoreaCollege of Veterinary Medicine, Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Republic of KoreaCollege of Veterinary Medicine, Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Republic of KoreaLaboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 545431, Republic of KoreaPorcine epidemic diarrhea (PED) is a highly contagious disease that has been reported annually in several Asian countries, causing significant economic losses to the swine livestock industry. Although vaccines against the porcine epidemic diarrhea virus (PEDV) are available, their efficacy remains questionable due to limitations such as viral genome mutation and insufficient intestinal mucosal immunity. Therefore, the development of a safe and effective vaccine is necessary. In this study, a virulent Korean strain of PEDV, CKT-7, was isolated from a piglet with severe diarrhea, and six different conditions were employed for serial passage of the strain in a cell culture system to generate effective live attenuated vaccine (LAV) candidates. The characteristics of these strains were analyzed in vitro and in vivo, and the CKT-7 N strain was identified as the most effective vaccine candidate, with a viral titer peak of 8.67 ± 0.29 log<sub>10</sub>TCID<sub>50</sub>/mL, and no mortality or diarrhea symptoms were observed in five-day-old piglets. These results indicate that LAV candidates can be generated through serial passage with different culture conditions and provide valuable insights into the development of a highly effective LAV against PEDV.https://www.mdpi.com/2076-393X/11/5/923porcine epidemic diarrhea viruslive attenuated vaccinepathogenicity |
| spellingShingle | Dae-Min Kim Sung-Hyun Moon Seung-Chai Kim Ho-Seong Cho Dongseob Tark Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity Vaccines porcine epidemic diarrhea virus live attenuated vaccine pathogenicity |
| title | Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity |
| title_full | Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity |
| title_fullStr | Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity |
| title_full_unstemmed | Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity |
| title_short | Development of Effective PEDV Vaccine Candidates Based on Viral Culture and Protease Activity |
| title_sort | development of effective pedv vaccine candidates based on viral culture and protease activity |
| topic | porcine epidemic diarrhea virus live attenuated vaccine pathogenicity |
| url | https://www.mdpi.com/2076-393X/11/5/923 |
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