Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects
<i>Lentinula edodes</i>, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of shiitake cultivation, namely spent mushroom substrate (SMS), to explore its nutraceutical properties. The SMS sample...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/12/1200 |
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| author | Filipa Baptista Joana Campos Valéria Costa-Silva Ana Rita Pinto Maria José Saavedra Luis Mendes Ferreira Miguel Rodrigues Ana Novo Barros |
| author_facet | Filipa Baptista Joana Campos Valéria Costa-Silva Ana Rita Pinto Maria José Saavedra Luis Mendes Ferreira Miguel Rodrigues Ana Novo Barros |
| author_sort | Filipa Baptista |
| collection | DOAJ |
| description | <i>Lentinula edodes</i>, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of shiitake cultivation, namely spent mushroom substrate (SMS), to explore its nutraceutical properties. The SMS samples were collected and subjected to different extraction methods, namely short or long agitation, and ultrasound-assisted extractions using different temperatures and distilled water or a 50% (<i>v</i>/<i>v</i>) ethanol as solvents. The extracts were tested for phenolic content (total phenols, <i>ortho</i>-diphenols, and flavonoids), antioxidant capacity (DPPH, 2,2-diphenyl-1 picrylhydrazyl; ABTS, 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; and FRAP, ferric reducing antioxidant power), and antibacterial activity. The different extraction methods revealed substantial variations (<i>p</i> < 0.05) in phenolic composition and antioxidant capacity. The highest phenolic content and antioxidant capacity were achieved using 24 h extraction, agitation, 50 °C, and ethanol as the solvent. Furthermore, the extracted compounds displayed antibacterial activity in specific tested bacterial strains. This study highlights the nutraceutical potential of <i>L. edodes</i>’ SMS, positioning it as a valuable dietary supplement for animal nutrition, with emphasis on its prebiotic properties. Hence, this research unveils the promising health benefits of SMS in both human and animal nutrition. |
| first_indexed | 2024-03-08T20:36:37Z |
| format | Article |
| id | doaj.art-2d0a2791c75545c587cc85930c8e3a55 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T20:36:37Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-2d0a2791c75545c587cc85930c8e3a552023-12-22T14:19:29ZengMDPI AGJournal of Fungi2309-608X2023-12-01912120010.3390/jof9121200Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial EffectsFilipa Baptista0Joana Campos1Valéria Costa-Silva2Ana Rita Pinto3Maria José Saavedra4Luis Mendes Ferreira5Miguel Rodrigues6Ana Novo Barros7Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCECAV—Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal<i>Lentinula edodes</i>, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of shiitake cultivation, namely spent mushroom substrate (SMS), to explore its nutraceutical properties. The SMS samples were collected and subjected to different extraction methods, namely short or long agitation, and ultrasound-assisted extractions using different temperatures and distilled water or a 50% (<i>v</i>/<i>v</i>) ethanol as solvents. The extracts were tested for phenolic content (total phenols, <i>ortho</i>-diphenols, and flavonoids), antioxidant capacity (DPPH, 2,2-diphenyl-1 picrylhydrazyl; ABTS, 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; and FRAP, ferric reducing antioxidant power), and antibacterial activity. The different extraction methods revealed substantial variations (<i>p</i> < 0.05) in phenolic composition and antioxidant capacity. The highest phenolic content and antioxidant capacity were achieved using 24 h extraction, agitation, 50 °C, and ethanol as the solvent. Furthermore, the extracted compounds displayed antibacterial activity in specific tested bacterial strains. This study highlights the nutraceutical potential of <i>L. edodes</i>’ SMS, positioning it as a valuable dietary supplement for animal nutrition, with emphasis on its prebiotic properties. Hence, this research unveils the promising health benefits of SMS in both human and animal nutrition.https://www.mdpi.com/2309-608X/9/12/1200<i>Lentinula edodes</i>spent mushroom substrateextraction methodsnutraceuticalsprebiotic supplementationvaluable by-product resources |
| spellingShingle | Filipa Baptista Joana Campos Valéria Costa-Silva Ana Rita Pinto Maria José Saavedra Luis Mendes Ferreira Miguel Rodrigues Ana Novo Barros Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects Journal of Fungi <i>Lentinula edodes</i> spent mushroom substrate extraction methods nutraceuticals prebiotic supplementation valuable by-product resources |
| title | Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects |
| title_full | Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects |
| title_fullStr | Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects |
| title_full_unstemmed | Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects |
| title_short | Nutraceutical Potential of <i>Lentinula edodes</i>’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects |
| title_sort | nutraceutical potential of i lentinula edodes i spent mushroom substrate a comprehensive study on phenolic composition antioxidant activity and antibacterial effects |
| topic | <i>Lentinula edodes</i> spent mushroom substrate extraction methods nutraceuticals prebiotic supplementation valuable by-product resources |
| url | https://www.mdpi.com/2309-608X/9/12/1200 |
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