Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis
The response to the synaptic release of γ-Aminobutyric acid (GABA) in the central nervous system is mediated by the GABAB receptor, inter alia dependent on the formation of receptor oligomers. Spatial intensity distribution analysis (SpIDA) is an image analysis method measuring the oligomerization a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2017-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2017-0141 |
| _version_ | 1797848693228961792 |
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| author | Bienzeisler Jonas Landry Marc Llorente Alberto Hüttmann Gereon |
| author_facet | Bienzeisler Jonas Landry Marc Llorente Alberto Hüttmann Gereon |
| author_sort | Bienzeisler Jonas |
| collection | DOAJ |
| description | The response to the synaptic release of γ-Aminobutyric acid (GABA) in the central nervous system is mediated by the GABAB receptor, inter alia dependent on the formation of receptor oligomers. Spatial intensity distribution analysis (SpIDA) is an image analysis method measuring the oligomerization and density of proteins. It was used to quantify the oligomerization of GABAB receptors in the rodent spinal cord under conditions of chronic pain and inflammation. SpIDA reports a lowered density of tetrameric GABAB receptor entities in GABAergic neurons of the dorsal horn under these conditions compared to sham samples. The soma of neurons in the ventral horn is shown to be populated by single entities of the GABAB receptor. The findings underline the role of receptor oligomerization, as well as the membrane environment in regulating receptor function. |
| first_indexed | 2024-04-09T18:32:46Z |
| format | Article |
| id | doaj.art-2d0b591b9a0543da819823982cd3d443 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-09T18:32:46Z |
| publishDate | 2017-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-2d0b591b9a0543da819823982cd3d4432023-04-11T17:07:14ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042017-09-013266967310.1515/cdbme-2017-0141cdbme-2017-0141Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysisBienzeisler Jonas0Landry Marc1Llorente Alberto2Hüttmann Gereon3Universität zu Lübeck, Medizinische Ingenieurwissenschaft, Lübeck, GermanyUniversité de Bordeaux, IINS, Bordeaux, FranceUniversidad del País Vasco, Bilbao, SpainUniversität zu Lübeck, Institut für Biomedizinische Optik, Lübeck, GermanyThe response to the synaptic release of γ-Aminobutyric acid (GABA) in the central nervous system is mediated by the GABAB receptor, inter alia dependent on the formation of receptor oligomers. Spatial intensity distribution analysis (SpIDA) is an image analysis method measuring the oligomerization and density of proteins. It was used to quantify the oligomerization of GABAB receptors in the rodent spinal cord under conditions of chronic pain and inflammation. SpIDA reports a lowered density of tetrameric GABAB receptor entities in GABAergic neurons of the dorsal horn under these conditions compared to sham samples. The soma of neurons in the ventral horn is shown to be populated by single entities of the GABAB receptor. The findings underline the role of receptor oligomerization, as well as the membrane environment in regulating receptor function.https://doi.org/10.1515/cdbme-2017-0141spatial intensity distribution analysisgabab receptoroligomerizationchronic pain |
| spellingShingle | Bienzeisler Jonas Landry Marc Llorente Alberto Hüttmann Gereon Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis Current Directions in Biomedical Engineering spatial intensity distribution analysis gabab receptor oligomerization chronic pain |
| title | Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| title_full | Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| title_fullStr | Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| title_full_unstemmed | Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| title_short | Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| title_sort | analysis of the in vivo gabab receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis |
| topic | spatial intensity distribution analysis gabab receptor oligomerization chronic pain |
| url | https://doi.org/10.1515/cdbme-2017-0141 |
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