Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia
Summary: Arboviruses are public health threats that cause explosive outbreaks. Major determinants of arbovirus transmission, geographic spread, and pathogenesis are the magnitude and duration of viremia in vertebrate hosts. Previously, we determined that multiple alphaviruses are cleared efficiently...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723004291 |
| _version_ | 1797842424380260352 |
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| author | Frances S. Li Kathryn S. Carpentier David W. Hawman Cormac J. Lucas Stephanie E. Ander Heinz Feldmann Thomas E. Morrison |
| author_facet | Frances S. Li Kathryn S. Carpentier David W. Hawman Cormac J. Lucas Stephanie E. Ander Heinz Feldmann Thomas E. Morrison |
| author_sort | Frances S. Li |
| collection | DOAJ |
| description | Summary: Arboviruses are public health threats that cause explosive outbreaks. Major determinants of arbovirus transmission, geographic spread, and pathogenesis are the magnitude and duration of viremia in vertebrate hosts. Previously, we determined that multiple alphaviruses are cleared efficiently from murine circulation by the scavenger receptor MARCO (Macrophage receptor with collagenous structure). Here, we define biochemical features on chikungunya (CHIKV), o’nyong ’nyong (ONNV), and Ross River (RRV) viruses required for MARCO-dependent clearance in vivo. In vitro, MARCO expression promotes binding and internalization of CHIKV, ONNV, and RRV via the scavenger receptor cysteine-rich (SRCR) domain. Furthermore, we observe species-specific effects of the MARCO SRCR domain on CHIKV internalization, where those from known amplification hosts fail to promote CHIKV internalization. Consistent with this observation, CHIKV is inefficiently cleared from the circulation of rhesus macaques in contrast with mice. These findings suggest a role for MARCO in determining whether a vertebrate serves as an amplification or dead-end host following CHIKV infection. |
| first_indexed | 2024-04-09T16:47:41Z |
| format | Article |
| id | doaj.art-2d0c7a1f21d4487fb3d8295abcdea33a |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-09T16:47:41Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-2d0c7a1f21d4487fb3d8295abcdea33a2023-04-22T06:20:51ZengElsevierCell Reports2211-12472023-05-01425112418Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremiaFrances S. Li0Kathryn S. Carpentier1David W. Hawman2Cormac J. Lucas3Stephanie E. Ander4Heinz Feldmann5Thomas E. Morrison6Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA; Corresponding authorSummary: Arboviruses are public health threats that cause explosive outbreaks. Major determinants of arbovirus transmission, geographic spread, and pathogenesis are the magnitude and duration of viremia in vertebrate hosts. Previously, we determined that multiple alphaviruses are cleared efficiently from murine circulation by the scavenger receptor MARCO (Macrophage receptor with collagenous structure). Here, we define biochemical features on chikungunya (CHIKV), o’nyong ’nyong (ONNV), and Ross River (RRV) viruses required for MARCO-dependent clearance in vivo. In vitro, MARCO expression promotes binding and internalization of CHIKV, ONNV, and RRV via the scavenger receptor cysteine-rich (SRCR) domain. Furthermore, we observe species-specific effects of the MARCO SRCR domain on CHIKV internalization, where those from known amplification hosts fail to promote CHIKV internalization. Consistent with this observation, CHIKV is inefficiently cleared from the circulation of rhesus macaques in contrast with mice. These findings suggest a role for MARCO in determining whether a vertebrate serves as an amplification or dead-end host following CHIKV infection.http://www.sciencedirect.com/science/article/pii/S2211124723004291CP: Microbiology |
| spellingShingle | Frances S. Li Kathryn S. Carpentier David W. Hawman Cormac J. Lucas Stephanie E. Ander Heinz Feldmann Thomas E. Morrison Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia Cell Reports CP: Microbiology |
| title | Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia |
| title_full | Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia |
| title_fullStr | Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia |
| title_full_unstemmed | Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia |
| title_short | Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia |
| title_sort | species specific marco alphavirus interactions dictate chikungunya virus viremia |
| topic | CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723004291 |
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