Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils
Sample pretreatment is a vital step in the detection of mycotoxins, and traditional pretreatment methods are time-consuming, labor-intensive and generate much organic waste liquid. In this work, an automatic, high-throughput and environmentally friendly pretreatment method is proposed. Immunomagneti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/5/337 |
| _version_ | 1797598143771049984 |
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| author | Baoxia Ni Jin Ye Zhihong Xuan Li Li Xiangrui Wen Zongwang Li Hongmei Liu Songxue Wang |
| author_facet | Baoxia Ni Jin Ye Zhihong Xuan Li Li Xiangrui Wen Zongwang Li Hongmei Liu Songxue Wang |
| author_sort | Baoxia Ni |
| collection | DOAJ |
| description | Sample pretreatment is a vital step in the detection of mycotoxins, and traditional pretreatment methods are time-consuming, labor-intensive and generate much organic waste liquid. In this work, an automatic, high-throughput and environmentally friendly pretreatment method is proposed. Immunomagnetic beads technology and dispersive liquid–liquid microextraction technology are combined, and the zearalenone in corn oils is directly purified and concentrated under the solubilization effects of surfactant. The proposed pretreatment method allows for the batch pretreatment of samples without pre-extraction using organic reagents, and almost no organic waste liquid is produced. Coupled with UPLC-FLD, an effective and accurate quantitative detection method for zearalenone is established. The recovery of spiked zearalenone in corn oils at different concentrations ranges from 85.7 to 89.0%, and the relative standard deviation is below 2.9%. The proposed pretreatment method overcomes the shortcomings of traditional pretreatment methods and has broad application prospects. |
| first_indexed | 2024-03-11T03:15:11Z |
| format | Article |
| id | doaj.art-2d0ccad0a8d34ee593adc02bb4fc5bbe |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T03:15:11Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-2d0ccad0a8d34ee593adc02bb4fc5bbe2023-11-18T03:33:37ZengMDPI AGToxins2072-66512023-05-0115533710.3390/toxins15050337Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn OilsBaoxia Ni0Jin Ye1Zhihong Xuan2Li Li3Xiangrui Wen4Zongwang Li5Hongmei Liu6Songxue Wang7Academy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Beijing 100037, ChinaSample pretreatment is a vital step in the detection of mycotoxins, and traditional pretreatment methods are time-consuming, labor-intensive and generate much organic waste liquid. In this work, an automatic, high-throughput and environmentally friendly pretreatment method is proposed. Immunomagnetic beads technology and dispersive liquid–liquid microextraction technology are combined, and the zearalenone in corn oils is directly purified and concentrated under the solubilization effects of surfactant. The proposed pretreatment method allows for the batch pretreatment of samples without pre-extraction using organic reagents, and almost no organic waste liquid is produced. Coupled with UPLC-FLD, an effective and accurate quantitative detection method for zearalenone is established. The recovery of spiked zearalenone in corn oils at different concentrations ranges from 85.7 to 89.0%, and the relative standard deviation is below 2.9%. The proposed pretreatment method overcomes the shortcomings of traditional pretreatment methods and has broad application prospects.https://www.mdpi.com/2072-6651/15/5/337immunomagnetic beadsdispersive liquid liquid microextractionpretreatmentzearalenonecorn oilsUPLC-FLD |
| spellingShingle | Baoxia Ni Jin Ye Zhihong Xuan Li Li Xiangrui Wen Zongwang Li Hongmei Liu Songxue Wang Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils Toxins immunomagnetic beads dispersive liquid liquid microextraction pretreatment zearalenone corn oils UPLC-FLD |
| title | Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils |
| title_full | Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils |
| title_fullStr | Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils |
| title_full_unstemmed | Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils |
| title_short | Automatic Pretreatment of Dispersive Liquid Liquid Microextraction Based on Immunomagnetic Beads Coupled with UPLC-FLD for the Determination of Zearalenone in Corn Oils |
| title_sort | automatic pretreatment of dispersive liquid liquid microextraction based on immunomagnetic beads coupled with uplc fld for the determination of zearalenone in corn oils |
| topic | immunomagnetic beads dispersive liquid liquid microextraction pretreatment zearalenone corn oils UPLC-FLD |
| url | https://www.mdpi.com/2072-6651/15/5/337 |
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