The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells
Background: Connective tissue subacromial bursa-derived progenitor cells (SBDCs) have been suggested as a potent biologic augment to promote healing of the repaired rotator cuff tendon. Maximizing the amount of retained progenitor cells at the tendon repair site is essential for ensuring an optimal...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | JSES International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666638323001755 |
| _version_ | 1797647737262440448 |
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| author | Lukas N. Muench, MD Lisa Tamburini, BS Danielle Kriscenski, BS Daniel P. Berthold, MD Marco-Christopher Rupp, MD Mark P. Cote, MS, DPT Mary Beth McCarthy, BS Augustus D. Mazzocca, MS, MD |
| author_facet | Lukas N. Muench, MD Lisa Tamburini, BS Danielle Kriscenski, BS Daniel P. Berthold, MD Marco-Christopher Rupp, MD Mark P. Cote, MS, DPT Mary Beth McCarthy, BS Augustus D. Mazzocca, MS, MD |
| author_sort | Lukas N. Muench, MD |
| collection | DOAJ |
| description | Background: Connective tissue subacromial bursa-derived progenitor cells (SBDCs) have been suggested as a potent biologic augment to promote healing of the repaired rotator cuff tendon. Maximizing the amount of retained progenitor cells at the tendon repair site is essential for ensuring an optimal healing environment, warranting a search for proadhesive and proliferative adjuvants. The purpose was to evaluate the effect of magnesium (Mg), platelet-rich plasma (PRP), and a combination of both adjuvants on the in vitro cellular adhesion and proliferation potential of SBDCs on suture material commonly used in rotator cuff surgery. Methods: SBDCs were isolated from subacromial bursa samples harvested during rotator cuff repair and cultured in growth media. Commercially available collagen-coated nonabsorbable flat-braided suture was cut into 1-inch pieces, placed into 48-well culture dishes, and sterilized under ultraviolet light. Either a one-time dose of 5 mM sterile Mg, 0.2 mL of PRP, or a combination of both adjuvants was added, while a group without treatment served as a negative control. Cellular proliferation and adhesion assays on suture material were performed for each treatment condition. Results: Augmenting the suture with Mg resulted in a significantly increased cellular adhesion (total number of attached cells) of SBDCs compared to PRP alone (31,527 ± 19,884 vs. 13,619 ± 8808; P < .001), no treatment (31,527 ± 19,884 vs. 21,643 ± 8194; P = .016), and combination of both adjuvants (31,527 ± 19,884 vs. 17,121 ± 11,935; P < .001). Further, augmentation with Mg achieved a significant increase in cellular proliferation (absorbance) of SBDCs on suture material when compared to the PRP (0.516 ± 0.207 vs. 0.424 ± 0.131; P = .001) and no treatment (0.516 ± 0.207 vs. 0.383 ± 0.094; P < .001) group. The combination of Mg and PRP showed a significantly higher proliferation potential compared to PRP alone (0.512 ± 0.194 vs. 0.424 ± 0.131; P = .001) and no treatment (0.512 ± 0.194 vs. 0.383 ± 0.094; P < .001). There were no significant differences in the remaining intergroup comparisons (P > .05, respectively). Conclusion: Augmenting suture material with Mg resulted in a significantly increased cellular adhesion of SBDCs compared to untreated suture material, as well as augmentation with PRP alone or a combination of both adjuvants. Further, Mg with or without PRP augmentation achieved a significant increase in the cellular proliferation of SBDCs on suture material compared to untreated sutures and augmentation with PRP alone. Application of Mg may be a clinically feasible approach to optimizing the use of SBDCs as a biological augment in rotator cuff repair, while combined augmentation with PRP may harness the full potential for optimized tissue recovery due to the high concentration of PRP-derived growth factors. |
| first_indexed | 2024-03-11T15:21:47Z |
| format | Article |
| id | doaj.art-2d0eb50de5de4cf6964637b5386c85fb |
| institution | Directory Open Access Journal |
| issn | 2666-6383 |
| language | English |
| last_indexed | 2024-03-11T15:21:47Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JSES International |
| spelling | doaj.art-2d0eb50de5de4cf6964637b5386c85fb2023-10-28T05:09:50ZengElsevierJSES International2666-63832023-11-017623672372The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cellsLukas N. Muench, MD0Lisa Tamburini, BS1Danielle Kriscenski, BS2Daniel P. Berthold, MD3Marco-Christopher Rupp, MD4Mark P. Cote, MS, DPT5Mary Beth McCarthy, BS6Augustus D. Mazzocca, MS, MD7Department of Sports Orthopaedics, Technical University of Munich, Munich, Germany; Corresponding author: Lukas N. Muench, MD, Department of Sports Orthopaedics, Technical University of Munich, Ismaninger Str. 22, 81675 München, Germany.Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USADepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USADepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, LMU, Munich, GermanyDepartment of Sports Orthopaedics, Technical University of Munich, Munich, GermanyDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USADepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USAMassachusetts General Hospital, Massachusetts General Brigham, Harvard Medical School, Boston, MA, USABackground: Connective tissue subacromial bursa-derived progenitor cells (SBDCs) have been suggested as a potent biologic augment to promote healing of the repaired rotator cuff tendon. Maximizing the amount of retained progenitor cells at the tendon repair site is essential for ensuring an optimal healing environment, warranting a search for proadhesive and proliferative adjuvants. The purpose was to evaluate the effect of magnesium (Mg), platelet-rich plasma (PRP), and a combination of both adjuvants on the in vitro cellular adhesion and proliferation potential of SBDCs on suture material commonly used in rotator cuff surgery. Methods: SBDCs were isolated from subacromial bursa samples harvested during rotator cuff repair and cultured in growth media. Commercially available collagen-coated nonabsorbable flat-braided suture was cut into 1-inch pieces, placed into 48-well culture dishes, and sterilized under ultraviolet light. Either a one-time dose of 5 mM sterile Mg, 0.2 mL of PRP, or a combination of both adjuvants was added, while a group without treatment served as a negative control. Cellular proliferation and adhesion assays on suture material were performed for each treatment condition. Results: Augmenting the suture with Mg resulted in a significantly increased cellular adhesion (total number of attached cells) of SBDCs compared to PRP alone (31,527 ± 19,884 vs. 13,619 ± 8808; P < .001), no treatment (31,527 ± 19,884 vs. 21,643 ± 8194; P = .016), and combination of both adjuvants (31,527 ± 19,884 vs. 17,121 ± 11,935; P < .001). Further, augmentation with Mg achieved a significant increase in cellular proliferation (absorbance) of SBDCs on suture material when compared to the PRP (0.516 ± 0.207 vs. 0.424 ± 0.131; P = .001) and no treatment (0.516 ± 0.207 vs. 0.383 ± 0.094; P < .001) group. The combination of Mg and PRP showed a significantly higher proliferation potential compared to PRP alone (0.512 ± 0.194 vs. 0.424 ± 0.131; P = .001) and no treatment (0.512 ± 0.194 vs. 0.383 ± 0.094; P < .001). There were no significant differences in the remaining intergroup comparisons (P > .05, respectively). Conclusion: Augmenting suture material with Mg resulted in a significantly increased cellular adhesion of SBDCs compared to untreated suture material, as well as augmentation with PRP alone or a combination of both adjuvants. Further, Mg with or without PRP augmentation achieved a significant increase in the cellular proliferation of SBDCs on suture material compared to untreated sutures and augmentation with PRP alone. Application of Mg may be a clinically feasible approach to optimizing the use of SBDCs as a biological augment in rotator cuff repair, while combined augmentation with PRP may harness the full potential for optimized tissue recovery due to the high concentration of PRP-derived growth factors.http://www.sciencedirect.com/science/article/pii/S2666638323001755Subacromial bursaProgenitor cellsPlatelet-rich plasmaMagnesiumBiologic augmentationCellular adhesion |
| spellingShingle | Lukas N. Muench, MD Lisa Tamburini, BS Danielle Kriscenski, BS Daniel P. Berthold, MD Marco-Christopher Rupp, MD Mark P. Cote, MS, DPT Mary Beth McCarthy, BS Augustus D. Mazzocca, MS, MD The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells JSES International Subacromial bursa Progenitor cells Platelet-rich plasma Magnesium Biologic augmentation Cellular adhesion |
| title | The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells |
| title_full | The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells |
| title_fullStr | The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells |
| title_full_unstemmed | The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells |
| title_short | The effect of augmenting suture material with magnesium and platelet-rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa-derived progenitor cells |
| title_sort | effect of augmenting suture material with magnesium and platelet rich plasma on the in vitro adhesion and proliferation potential of subacromial bursa derived progenitor cells |
| topic | Subacromial bursa Progenitor cells Platelet-rich plasma Magnesium Biologic augmentation Cellular adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S2666638323001755 |
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