Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages
Seeds from non-drug varieties of hemp (<i>Cannabis sativa</i> L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and...
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/15/2366 |
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| author | Carmen M. Claro-Cala Elena Grao-Cruces Rocio Toscano Maria C. Millan-Linares Sergio Montserrat-de la Paz Maria E. Martin |
| author_facet | Carmen M. Claro-Cala Elena Grao-Cruces Rocio Toscano Maria C. Millan-Linares Sergio Montserrat-de la Paz Maria E. Martin |
| author_sort | Carmen M. Claro-Cala |
| collection | DOAJ |
| description | Seeds from non-drug varieties of hemp (<i>Cannabis sativa</i> L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10–100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14<sup>+</sup>CD16<sup>++</sup> monocyte phenotype and toward macrophage M2 (CD200R<sup>high</sup> and MRC-1<sup>high</sup>), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage. |
| first_indexed | 2024-03-09T05:24:03Z |
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| last_indexed | 2024-03-09T05:24:03Z |
| publishDate | 2022-08-01 |
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| series | Foods |
| spelling | doaj.art-2d125a00307044d09e9bdef4173935e02023-12-03T12:38:04ZengMDPI AGFoods2304-81582022-08-011115236610.3390/foods11152366Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-MacrophagesCarmen M. Claro-Cala0Elena Grao-Cruces1Rocio Toscano2Maria C. Millan-Linares3Sergio Montserrat-de la Paz4Maria E. Martin5Department of Pharmacology, Pediatric and Radiology, Faculty of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, SpainDepartment of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, SpainDepartment of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, SpainDepartment of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, SpainDepartment of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, SpainDepartment of Cell Biology, Faculty of Biology, Universidad de Sevilla, Av. Reina Mercedes s/n, 41012 Seville, SpainSeeds from non-drug varieties of hemp (<i>Cannabis sativa</i> L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10–100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14<sup>+</sup>CD16<sup>++</sup> monocyte phenotype and toward macrophage M2 (CD200R<sup>high</sup> and MRC-1<sup>high</sup>), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage.https://www.mdpi.com/2304-8158/11/15/2366phytolnutraceuticalsunsaponifiablehemp seed oilfunctional foodsimmunonutrition |
| spellingShingle | Carmen M. Claro-Cala Elena Grao-Cruces Rocio Toscano Maria C. Millan-Linares Sergio Montserrat-de la Paz Maria E. Martin Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages Foods phytol nutraceuticals unsaponifiable hemp seed oil functional foods immunonutrition |
| title | Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages |
| title_full | Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages |
| title_fullStr | Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages |
| title_full_unstemmed | Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages |
| title_short | Acyclic Diterpene Phytol from Hemp Seed Oil (<i>Cannabis sativa</i> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages |
| title_sort | acyclic diterpene phytol from hemp seed oil i cannabis sativa i l exerts anti inflammatory activity on primary human monocytes macrophages |
| topic | phytol nutraceuticals unsaponifiable hemp seed oil functional foods immunonutrition |
| url | https://www.mdpi.com/2304-8158/11/15/2366 |
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