Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A
Objective: Current evidence has revealed a significant association between bullous pemphigoid (BP) and neurological diseases (ND), including stroke, but the incidence of BP autoantibodies in patients with stroke has not previously been investigated. Our study aimed to assess BP antigen-specific anti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00236/full |
| _version_ | 1817998071925047296 |
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| author | Yanan Wang Xuming Mao Di Wang Christoph M. Hammers Aimee S. Payne Yiman Wang Hongzhong Jin Bin Peng Li Li |
| author_facet | Yanan Wang Xuming Mao Di Wang Christoph M. Hammers Aimee S. Payne Yiman Wang Hongzhong Jin Bin Peng Li Li |
| author_sort | Yanan Wang |
| collection | DOAJ |
| description | Objective: Current evidence has revealed a significant association between bullous pemphigoid (BP) and neurological diseases (ND), including stroke, but the incidence of BP autoantibodies in patients with stroke has not previously been investigated. Our study aimed to assess BP antigen-specific antibodies in stroke patients.Design: One hundred patients with stroke and 100 matched healthy controls were randomly selected for measurement of anti-BP180/BP230 IgG autoantibodies by enzyme-linked immunosorbent assay (ELISA), salt-split indirect immunofluorescence (IIF), and immunoblotting against human cutaneous BP180 and BP180-NC16A.Results: Anti-BP180 autoantibodies were found in 14 (14.0%) patients with stroke and 5 (5.0%) of controls by ELISA (p < 0.05). Sera from 13 (13.0%) patients with stroke and 3 (3.0%) controls reacted with 180-kDa proteins from human epidermal extract (p < 0.05). 11 (11.0%) of stroke and 2 (2.0%) of control sera recognized the human recombinant full length BP180 and NC16A (p < 0.05). The anti-BP180-positive patients were significantly younger than the negative patients at the time of stroke (p < 0.001).Conclusion: Development of anti-BP180 autoantibodies occurs at a higher frequency after stroke, suggesting BP180 as a relatively common autoantigen after stroke and providing novel insights into BP pathogenesis in aging. |
| first_indexed | 2024-04-14T02:47:43Z |
| format | Article |
| id | doaj.art-2d169357baba441ebb86dad31510fd51 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-14T02:47:43Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2d169357baba441ebb86dad31510fd512022-12-22T02:16:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-02-011010.3389/fimmu.2019.00236432786Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16AYanan Wang0Xuming Mao1Di Wang2Christoph M. Hammers3Aimee S. Payne4Yiman Wang5Hongzhong Jin6Bin Peng7Li Li8Department of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaDepartment of Dermatology, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaDepartment of Dermatology, University of Luebeck, Luebeck, GermanyDepartment of Dermatology, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaDepartment of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaDepartment of Neurology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaDepartment of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy Medical Science, Beijing, ChinaObjective: Current evidence has revealed a significant association between bullous pemphigoid (BP) and neurological diseases (ND), including stroke, but the incidence of BP autoantibodies in patients with stroke has not previously been investigated. Our study aimed to assess BP antigen-specific antibodies in stroke patients.Design: One hundred patients with stroke and 100 matched healthy controls were randomly selected for measurement of anti-BP180/BP230 IgG autoantibodies by enzyme-linked immunosorbent assay (ELISA), salt-split indirect immunofluorescence (IIF), and immunoblotting against human cutaneous BP180 and BP180-NC16A.Results: Anti-BP180 autoantibodies were found in 14 (14.0%) patients with stroke and 5 (5.0%) of controls by ELISA (p < 0.05). Sera from 13 (13.0%) patients with stroke and 3 (3.0%) controls reacted with 180-kDa proteins from human epidermal extract (p < 0.05). 11 (11.0%) of stroke and 2 (2.0%) of control sera recognized the human recombinant full length BP180 and NC16A (p < 0.05). The anti-BP180-positive patients were significantly younger than the negative patients at the time of stroke (p < 0.001).Conclusion: Development of anti-BP180 autoantibodies occurs at a higher frequency after stroke, suggesting BP180 as a relatively common autoantigen after stroke and providing novel insights into BP pathogenesis in aging.https://www.frontiersin.org/article/10.3389/fimmu.2019.00236/fullBP180anti-BP180 autoantibodiesBP180-NC16Abullous pemphigoidstroke |
| spellingShingle | Yanan Wang Xuming Mao Di Wang Christoph M. Hammers Aimee S. Payne Yiman Wang Hongzhong Jin Bin Peng Li Li Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A Frontiers in Immunology BP180 anti-BP180 autoantibodies BP180-NC16A bullous pemphigoid stroke |
| title | Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A |
| title_full | Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A |
| title_fullStr | Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A |
| title_full_unstemmed | Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A |
| title_short | Anti-BP180 Autoantibodies Are Present in Stroke and Recognize Human Cutaneous BP180 and BP180-NC16A |
| title_sort | anti bp180 autoantibodies are present in stroke and recognize human cutaneous bp180 and bp180 nc16a |
| topic | BP180 anti-BP180 autoantibodies BP180-NC16A bullous pemphigoid stroke |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00236/full |
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