Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America
Leptographium wageneri is a native fungal pathogen in western North America that causes black stain root disease (BSRD) of conifers. Three host-specialized varieties of this pathogen were previously described: L. wageneri var. wageneri on pinyon pines (Pinus monophylla and P. edulis); L. wageneri va...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1286157/full |
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| author | Daram Choi Daram Choi Thomas C. Harrington David C. Shaw Jane E. Stewart Ned B. Klopfenstein Duncan R. Kroese Mee-Sook Kim |
| author_facet | Daram Choi Daram Choi Thomas C. Harrington David C. Shaw Jane E. Stewart Ned B. Klopfenstein Duncan R. Kroese Mee-Sook Kim |
| author_sort | Daram Choi |
| collection | DOAJ |
| description | Leptographium wageneri is a native fungal pathogen in western North America that causes black stain root disease (BSRD) of conifers. Three host-specialized varieties of this pathogen were previously described: L. wageneri var. wageneri on pinyon pines (Pinus monophylla and P. edulis); L. wageneri var. ponderosum, primarily on hard pines (e.g., P. ponderosa, P. jeffreyi); and L. wageneri var. pseudotsugae on Douglas-fir (Pseudotsuga menziesii). Morphological, physiological, and ecological differences among the three pathogen varieties have been previously determined; however, DNA-based characterization and analyses are needed to determine the genetic relationships among these varieties. The objective of this study was to use DNA sequences of 10 gene regions to assess phylogenetic relationships among L. wageneri isolates collected from different hosts. The multigene phylogenetic analyses, based on maximum likelihood and Bayesian inference, strongly supported species-level separation of the three L. wageneri varieties. These results, in conjunction with previously established phenotypic differences, support the elevation of L. wageneri var. ponderosum and L. wageneri var. pseudotsugae to the species level as L. ponderosum comb. nov. and L. pseudotsugae comb. nov., respectively, while maintaining L. wageneri var. wageneri as Leptographium wageneri. Characterization of the three Leptographium species, each with distinct host ranges, provides a baseline to further understand the ecological interactions and evolutionary relationships of these forest pathogens, which informs management of black stain root disease. |
| first_indexed | 2024-03-08T21:13:08Z |
| format | Article |
| id | doaj.art-2d17f76e7e30447d8ac3f6087019c6cf |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T21:13:08Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2d17f76e7e30447d8ac3f6087019c6cf2023-12-22T04:21:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-12-011410.3389/fpls.2023.12861571286157Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North AmericaDaram Choi0Daram Choi1Thomas C. Harrington2David C. Shaw3Jane E. Stewart4Ned B. Klopfenstein5Duncan R. Kroese6Mee-Sook Kim7Department of Forest Engineering, Resources and Management, Oregon State University, Corvallis, OR, United StatesPacific Northwest Research Station, USDA Forest Service, Corvallis, OR, United StatesDepartment of Plant Pathology, Entomology and Microbiology, Iowa State University, Ames, IA, United StatesDepartment of Forest Engineering, Resources and Management, Oregon State University, Corvallis, OR, United StatesDepartment of Agricultural Biology, Colorado State University, Fort Collins, CO, United StatesRocky Mountain Research Station, USDA Forest Service, Moscow, ID, United StatesPacific Northwest Research Station, USDA Forest Service, Corvallis, OR, United StatesPacific Northwest Research Station, USDA Forest Service, Corvallis, OR, United StatesLeptographium wageneri is a native fungal pathogen in western North America that causes black stain root disease (BSRD) of conifers. Three host-specialized varieties of this pathogen were previously described: L. wageneri var. wageneri on pinyon pines (Pinus monophylla and P. edulis); L. wageneri var. ponderosum, primarily on hard pines (e.g., P. ponderosa, P. jeffreyi); and L. wageneri var. pseudotsugae on Douglas-fir (Pseudotsuga menziesii). Morphological, physiological, and ecological differences among the three pathogen varieties have been previously determined; however, DNA-based characterization and analyses are needed to determine the genetic relationships among these varieties. The objective of this study was to use DNA sequences of 10 gene regions to assess phylogenetic relationships among L. wageneri isolates collected from different hosts. The multigene phylogenetic analyses, based on maximum likelihood and Bayesian inference, strongly supported species-level separation of the three L. wageneri varieties. These results, in conjunction with previously established phenotypic differences, support the elevation of L. wageneri var. ponderosum and L. wageneri var. pseudotsugae to the species level as L. ponderosum comb. nov. and L. pseudotsugae comb. nov., respectively, while maintaining L. wageneri var. wageneri as Leptographium wageneri. Characterization of the three Leptographium species, each with distinct host ranges, provides a baseline to further understand the ecological interactions and evolutionary relationships of these forest pathogens, which informs management of black stain root disease.https://www.frontiersin.org/articles/10.3389/fpls.2023.1286157/fullLeptographiumphylogenygenetic characterizationforest pathogentaxonomy |
| spellingShingle | Daram Choi Daram Choi Thomas C. Harrington David C. Shaw Jane E. Stewart Ned B. Klopfenstein Duncan R. Kroese Mee-Sook Kim Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America Frontiers in Plant Science Leptographium phylogeny genetic characterization forest pathogen taxonomy |
| title | Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America |
| title_full | Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America |
| title_fullStr | Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America |
| title_full_unstemmed | Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America |
| title_short | Phylogenetic analyses allow species-level recognition of Leptographium wageneri varieties that cause black stain root disease of conifers in western North America |
| title_sort | phylogenetic analyses allow species level recognition of leptographium wageneri varieties that cause black stain root disease of conifers in western north america |
| topic | Leptographium phylogeny genetic characterization forest pathogen taxonomy |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1286157/full |
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