Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration
In this study, hydrogels based on in situ crosslinking between oxidized alginate (OA) and N,O-carboxymethyl chitosan (NOCC) in combination with β-tricalcium phosphate (β-TCP) were prepared using different OA concentrations and OA to NOCC ratios. Various hydrogel characterizations were examined and r...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Science: Advanced Materials and Devices |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468217922000879 |
| _version_ | 1828299511090380800 |
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| author | Binh Thanh Vu Van My Hua Tuan-Ngan Tang Nhi Ngoc-Thao Dang Hang Thi-Thuy Cao Thang Bach Phan Hanh Thi-Kieu Ta Viet Hung Pham Quyen Ngoc Tran Thanh Dinh Le Toi Van Vo Hiep Thi Nguyen |
| author_facet | Binh Thanh Vu Van My Hua Tuan-Ngan Tang Nhi Ngoc-Thao Dang Hang Thi-Thuy Cao Thang Bach Phan Hanh Thi-Kieu Ta Viet Hung Pham Quyen Ngoc Tran Thanh Dinh Le Toi Van Vo Hiep Thi Nguyen |
| author_sort | Binh Thanh Vu |
| collection | DOAJ |
| description | In this study, hydrogels based on in situ crosslinking between oxidized alginate (OA) and N,O-carboxymethyl chitosan (NOCC) in combination with β-tricalcium phosphate (β-TCP) were prepared using different OA concentrations and OA to NOCC ratios. Various hydrogel characterizations were examined and revealed the strong effect of OA concentrations and NOCC contents on the ability of hydrogel formation, swelling degree, degradability and compression strength. Besides, Schiff's base crosslinking between aldehyde groups of OA and amino groups of NOCC, indicating hydrogel formation, was confirmed via Fourier transform infrared spectroscopy (FT-IR) spectra. The presence of β-TCP in hydrogels was also confirmed by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) images. Furthermore, most hydrogels were non-cytotoxic and expressed the capability for cells to penetrate, migrate and exchange nutrients and oxygen. Besides, further experiments demonstrated their in vivo biocompatibility. Generally, the results suggested that hydrogels based on 3% (w/v) OA, 3% (w/v) NOCC (OA:NOCC volume ratio is 1:3) and 20% (w/w) β-TCP have the potential for bone regeneration. |
| first_indexed | 2024-04-13T12:55:37Z |
| format | Article |
| id | doaj.art-2d184158ade046bf86016115972e3bbd |
| institution | Directory Open Access Journal |
| issn | 2468-2179 |
| language | English |
| last_indexed | 2024-04-13T12:55:37Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Science: Advanced Materials and Devices |
| spelling | doaj.art-2d184158ade046bf86016115972e3bbd2022-12-22T02:46:03ZengElsevierJournal of Science: Advanced Materials and Devices2468-21792022-12-0174100503Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regenerationBinh Thanh Vu0Van My Hua1Tuan-Ngan Tang2Nhi Ngoc-Thao Dang3Hang Thi-Thuy Cao4Thang Bach Phan5Hanh Thi-Kieu Ta6Viet Hung Pham7Quyen Ngoc Tran8Thanh Dinh Le9Toi Van Vo10Hiep Thi Nguyen11Tissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamTissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamTissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamTissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamNhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Khanh Hoa Province, Viet NamVietnam National University, Ho Chi Minh City, Viet Nam; Center for Innovative Materials and Architectures, Ho Chi Minh City, Viet Nam; Laboratory of Advanced Materials, University of Science, Ho Chi Minh City, Viet NamVietnam National University, Ho Chi Minh City, Viet Nam; Center for Innovative Materials and Architectures, Ho Chi Minh City, Viet Nam; Faculty of Materials Science and Technology, University of Science, Ho Chi Minh City, Viet NamKey Laboratory of Analytical Technology for Environmental Quality and Food Safety Control, University of Science, Vietnam National University, Hanoi, Viet NamInstitute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Viet NamThong Nhat Hospital, Ho Chi Minh City, Viet NamTissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet NamTissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet Nam; Corresponding author. Tissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, Viet Nam.In this study, hydrogels based on in situ crosslinking between oxidized alginate (OA) and N,O-carboxymethyl chitosan (NOCC) in combination with β-tricalcium phosphate (β-TCP) were prepared using different OA concentrations and OA to NOCC ratios. Various hydrogel characterizations were examined and revealed the strong effect of OA concentrations and NOCC contents on the ability of hydrogel formation, swelling degree, degradability and compression strength. Besides, Schiff's base crosslinking between aldehyde groups of OA and amino groups of NOCC, indicating hydrogel formation, was confirmed via Fourier transform infrared spectroscopy (FT-IR) spectra. The presence of β-TCP in hydrogels was also confirmed by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) images. Furthermore, most hydrogels were non-cytotoxic and expressed the capability for cells to penetrate, migrate and exchange nutrients and oxygen. Besides, further experiments demonstrated their in vivo biocompatibility. Generally, the results suggested that hydrogels based on 3% (w/v) OA, 3% (w/v) NOCC (OA:NOCC volume ratio is 1:3) and 20% (w/w) β-TCP have the potential for bone regeneration.http://www.sciencedirect.com/science/article/pii/S2468217922000879β-tricalcium phosphateBone regenerationIn situ crosslinking hydrogelN,O-carboxymethyl chitosanOxidized alginate |
| spellingShingle | Binh Thanh Vu Van My Hua Tuan-Ngan Tang Nhi Ngoc-Thao Dang Hang Thi-Thuy Cao Thang Bach Phan Hanh Thi-Kieu Ta Viet Hung Pham Quyen Ngoc Tran Thanh Dinh Le Toi Van Vo Hiep Thi Nguyen Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration Journal of Science: Advanced Materials and Devices β-tricalcium phosphate Bone regeneration In situ crosslinking hydrogel N,O-carboxymethyl chitosan Oxidized alginate |
| title | Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration |
| title_full | Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration |
| title_fullStr | Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration |
| title_full_unstemmed | Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration |
| title_short | Fabrication of in situ crosslinking hydrogels based on oxidized alginate/N,O-carboxymethyl chitosan/β-tricalcium phosphate for bone regeneration |
| title_sort | fabrication of in situ crosslinking hydrogels based on oxidized alginate n o carboxymethyl chitosan β tricalcium phosphate for bone regeneration |
| topic | β-tricalcium phosphate Bone regeneration In situ crosslinking hydrogel N,O-carboxymethyl chitosan Oxidized alginate |
| url | http://www.sciencedirect.com/science/article/pii/S2468217922000879 |
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