Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i>
<i>Rehmannia glutinosa</i> is an important medicinal plant that has long been used in Chinese traditional medicine. Acteoside, one of the bioactive components from <i>R. glutinosa</i>, possessed various pharmacological activities for human health; however, the molecular mecha...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/5/1634 |
| _version_ | 1827651084268601344 |
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| author | Yuanjun Li Yanan Wang Longyu Huang Chunrong Chen Na An Xiaoke Zheng |
| author_facet | Yuanjun Li Yanan Wang Longyu Huang Chunrong Chen Na An Xiaoke Zheng |
| author_sort | Yuanjun Li |
| collection | DOAJ |
| description | <i>Rehmannia glutinosa</i> is an important medicinal plant that has long been used in Chinese traditional medicine. Acteoside, one of the bioactive components from <i>R. glutinosa</i>, possessed various pharmacological activities for human health; however, the molecular mechanism of acteoside formation is not fully understood. In the current study, a novel tyrosine decarboxylase (designated as RgTyDC2) was identified from the <i>R. glutinosa</i> transcriptome. Biochemical analysis of RgTyDC2 showed RgTyDC2 uses tyrosine and dopa as the substrate to produce tyramine and dopamine, respectively, and it displays higher catalytic efficiency toward tyrosine than dopa. Moreover, the transcript level of <i>RgTyDC2</i> was consistent with the accumulation pattern of acteoside in <i>R. glutinosa</i>, supporting its possible role in the biosynthesis of acteoside in vivo. |
| first_indexed | 2024-03-09T20:28:54Z |
| format | Article |
| id | doaj.art-2d199825124941ad9bcf0047a7d96dd3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:28:54Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2d199825124941ad9bcf0047a7d96dd32023-11-23T23:27:27ZengMDPI AGMolecules1420-30492022-03-01275163410.3390/molecules27051634Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i>Yuanjun Li0Yanan Wang1Longyu Huang2Chunrong Chen3Na An4Xiaoke Zheng5College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaCollege of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaInstitute of Cotton Research, Chinese Academy of Agricultural Sciences (CAAS), Anyang 455000, ChinaSchool of Life Science, Henan University, Kaifeng 475004, ChinaCollege of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaCollege of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China<i>Rehmannia glutinosa</i> is an important medicinal plant that has long been used in Chinese traditional medicine. Acteoside, one of the bioactive components from <i>R. glutinosa</i>, possessed various pharmacological activities for human health; however, the molecular mechanism of acteoside formation is not fully understood. In the current study, a novel tyrosine decarboxylase (designated as RgTyDC2) was identified from the <i>R. glutinosa</i> transcriptome. Biochemical analysis of RgTyDC2 showed RgTyDC2 uses tyrosine and dopa as the substrate to produce tyramine and dopamine, respectively, and it displays higher catalytic efficiency toward tyrosine than dopa. Moreover, the transcript level of <i>RgTyDC2</i> was consistent with the accumulation pattern of acteoside in <i>R. glutinosa</i>, supporting its possible role in the biosynthesis of acteoside in vivo.https://www.mdpi.com/1420-3049/27/5/1634<i>Rehmannia glutinosa</i>acteosidetyrosine decarboxylase |
| spellingShingle | Yuanjun Li Yanan Wang Longyu Huang Chunrong Chen Na An Xiaoke Zheng Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> Molecules <i>Rehmannia glutinosa</i> acteoside tyrosine decarboxylase |
| title | Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> |
| title_full | Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> |
| title_fullStr | Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> |
| title_full_unstemmed | Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> |
| title_short | Identification and Functional Characterization of Tyrosine Decarboxylase from <i>Rehmannia glutinosa</i> |
| title_sort | identification and functional characterization of tyrosine decarboxylase from i rehmannia glutinosa i |
| topic | <i>Rehmannia glutinosa</i> acteoside tyrosine decarboxylase |
| url | https://www.mdpi.com/1420-3049/27/5/1634 |
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