Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation
Phosphophoryn (PP) and dentin sialoprotein (DSP) are two of the most abundant dentin matrix non-collagenous proteins, and are derived from dentin sialoprotein-phosphophoryn (DSP-PP) mRNA. Mutations in the DSP-PP gene are linked to dentinogenesis imperfecta II and III. Previously, we reported transie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/6/4/70 |
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| author | Shu-Feng Chuang Yu-Hsuan Chen Peter Ma Helena H. Ritchie |
| author_facet | Shu-Feng Chuang Yu-Hsuan Chen Peter Ma Helena H. Ritchie |
| author_sort | Shu-Feng Chuang |
| collection | DOAJ |
| description | Phosphophoryn (PP) and dentin sialoprotein (DSP) are two of the most abundant dentin matrix non-collagenous proteins, and are derived from dentin sialoprotein-phosphophoryn (DSP-PP) mRNA. Mutations in the DSP-PP gene are linked to dentinogenesis imperfecta II and III. Previously, we reported transient DSP-PP expression in preameloblast cells first, followed by co-expression in preameloblasts and young odontoblasts, and finally sustained expression in odontoblasts. This phenomenon raised the possibility that DSP/PP proteins secreted by preameloblasts might promote dental pulp cell migration toward the dental pulp border and promote dental pulp cell differentiation. To examine the effects of DSP/PP proteins on dental pulp cell development, we investigated:(1) native PP effects on dental pulpcell migration and matrix protein expression; and (2) recombinant DSP/PP protein effects on cell proliferation and differentiation. We found that PP promoted cell migration and the expression of high levels of Col type I and PP in dental pulp cells. The addition of recombinant DSP/PP proteins affected cell proliferation and differentiation in a dental pulp cell line. These findings strongly suggest that DSP/PP may modulate cell migration, cell proliferation and differentiation, thus leading to dentin formation. DSP/PP protein may be useful clinically for pulp tissue regeneration. |
| first_indexed | 2024-04-11T12:57:59Z |
| format | Article |
| id | doaj.art-2d1baf22d4954907baf412866d47c5ee |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-04-11T12:57:59Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-2d1baf22d4954907baf412866d47c5ee2022-12-22T04:23:01ZengMDPI AGDentistry Journal2304-67672018-12-01647010.3390/dj6040070dj6040070Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and DifferentiationShu-Feng Chuang0Yu-Hsuan Chen1Peter Ma2Helena H. Ritchie3Institute of Oral Medicine, National Cheng Kung University, Tainan 709, TaiwanInstitute of Oral Medicine, National Cheng Kung University, Tainan 709, TaiwanDepartment of Biomaterial Science, School of Dentitry, University of Michigan, Ann Arbor, MI 48109, USADepartment of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor MI 48109, USAPhosphophoryn (PP) and dentin sialoprotein (DSP) are two of the most abundant dentin matrix non-collagenous proteins, and are derived from dentin sialoprotein-phosphophoryn (DSP-PP) mRNA. Mutations in the DSP-PP gene are linked to dentinogenesis imperfecta II and III. Previously, we reported transient DSP-PP expression in preameloblast cells first, followed by co-expression in preameloblasts and young odontoblasts, and finally sustained expression in odontoblasts. This phenomenon raised the possibility that DSP/PP proteins secreted by preameloblasts might promote dental pulp cell migration toward the dental pulp border and promote dental pulp cell differentiation. To examine the effects of DSP/PP proteins on dental pulp cell development, we investigated:(1) native PP effects on dental pulpcell migration and matrix protein expression; and (2) recombinant DSP/PP protein effects on cell proliferation and differentiation. We found that PP promoted cell migration and the expression of high levels of Col type I and PP in dental pulp cells. The addition of recombinant DSP/PP proteins affected cell proliferation and differentiation in a dental pulp cell line. These findings strongly suggest that DSP/PP may modulate cell migration, cell proliferation and differentiation, thus leading to dentin formation. DSP/PP protein may be useful clinically for pulp tissue regeneration.https://www.mdpi.com/2304-6767/6/4/70phosphophoryn (PP)dentin sialoprotein (DSP)dentinogenesisdental pulp cellscell migrationdifferentiation |
| spellingShingle | Shu-Feng Chuang Yu-Hsuan Chen Peter Ma Helena H. Ritchie Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation Dentistry Journal phosphophoryn (PP) dentin sialoprotein (DSP) dentinogenesis dental pulp cells cell migration differentiation |
| title | Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation |
| title_full | Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation |
| title_fullStr | Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation |
| title_full_unstemmed | Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation |
| title_short | Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation |
| title_sort | phosphophoryn and dentin sialoprotein effects on dental pulp cell migration proliferation and differentiation |
| topic | phosphophoryn (PP) dentin sialoprotein (DSP) dentinogenesis dental pulp cells cell migration differentiation |
| url | https://www.mdpi.com/2304-6767/6/4/70 |
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