Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i>
In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/7/845 |
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| author | Sunitha Shiva Thilani Samarakoon Kaleb A. Lowe Charles Roach Hieu Sy Vu Madeline Colter Hollie Porras Caroline Hwang Mary R. Roth Pamela Tamura Maoyin Li Kathrin Schrick Jyoti Shah Xuemin Wang Haiyan Wang Ruth Welti |
| author_facet | Sunitha Shiva Thilani Samarakoon Kaleb A. Lowe Charles Roach Hieu Sy Vu Madeline Colter Hollie Porras Caroline Hwang Mary R. Roth Pamela Tamura Maoyin Li Kathrin Schrick Jyoti Shah Xuemin Wang Haiyan Wang Ruth Welti |
| author_sort | Sunitha Shiva |
| collection | DOAJ |
| description | In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in <i>Arabidopsis thaliana</i> leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected. |
| first_indexed | 2024-03-10T18:41:18Z |
| format | Article |
| id | doaj.art-2d1cf954bf494b8894f3295835a3cc04 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:41:18Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-2d1cf954bf494b8894f3295835a3cc042023-11-20T05:51:09ZengMDPI AGPlants2223-77472020-07-019784510.3390/plants9070845Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i>Sunitha Shiva0Thilani Samarakoon1Kaleb A. Lowe2Charles Roach3Hieu Sy Vu4Madeline Colter5Hollie Porras6Caroline Hwang7Mary R. Roth8Pamela Tamura9Maoyin Li10Kathrin Schrick11Jyoti Shah12Xuemin Wang13Haiyan Wang14Ruth Welti15Division of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADepartment of Biological Sciences, University of North Texas, Denton, TX 76203-5017, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USADonald Danforth Plant Science Center, St. Louis, MO 63132, USADepartment of Biological Sciences, University of North Texas, Denton, TX 76203-5017, USADepartment of Statistics, Kansas State University, Manhattan, KS 66506-0802, USADivision of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USAIn response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in <i>Arabidopsis thaliana</i> leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected.https://www.mdpi.com/2223-7747/9/7/845<i>Arabidopsis thaliana</i>heat stresslipidomicsoxidized lipidsacylated lipidsphosphatidic acid |
| spellingShingle | Sunitha Shiva Thilani Samarakoon Kaleb A. Lowe Charles Roach Hieu Sy Vu Madeline Colter Hollie Porras Caroline Hwang Mary R. Roth Pamela Tamura Maoyin Li Kathrin Schrick Jyoti Shah Xuemin Wang Haiyan Wang Ruth Welti Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> Plants <i>Arabidopsis thaliana</i> heat stress lipidomics oxidized lipids acylated lipids phosphatidic acid |
| title | Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> |
| title_full | Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> |
| title_fullStr | Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> |
| title_short | Leaf Lipid Alterations in Response to Heat Stress of <i>Arabidopsis thaliana</i> |
| title_sort | leaf lipid alterations in response to heat stress of i arabidopsis thaliana i |
| topic | <i>Arabidopsis thaliana</i> heat stress lipidomics oxidized lipids acylated lipids phosphatidic acid |
| url | https://www.mdpi.com/2223-7747/9/7/845 |
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