Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet
Polyphenols from the <i>Lonicera caerulea</i> L. berry have shown protective effects on experimental non-alcoholic fatty liver disease (NAFLD) in our previous studies. As endotoxins from gut bacteria are considered to be the major trigger of inflammation in NAFLD, this study aims to clar...
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/23/12/3213 |
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| author | Shusong Wu Ruizhi Hu Hironobu Nakano Keyu Chen Ming Liu Xi He Hongfu Zhang Jianhua He De-Xing Hou |
| author_facet | Shusong Wu Ruizhi Hu Hironobu Nakano Keyu Chen Ming Liu Xi He Hongfu Zhang Jianhua He De-Xing Hou |
| author_sort | Shusong Wu |
| collection | DOAJ |
| description | Polyphenols from the <i>Lonicera caerulea</i> L. berry have shown protective effects on experimental non-alcoholic fatty liver disease (NAFLD) in our previous studies. As endotoxins from gut bacteria are considered to be the major trigger of inflammation in NAFLD, this study aims to clarify the regulatory effects of <i>L. caerulea</i> L. berry polyphenols (LCBP) on gut microbiota in a high fat diet (HFD)-induced mouse model. C57BL/6N mice were fed with a normal diet, HFD, or HFD containing 0.5⁻1% of LCBP for 45 days. The results revealed that supplementation with LCBP decreased significantly the levels of IL-2, IL-6, MCP-1, and TNF-α in serum, as well as endotoxin levels in both serum and liver in HFD-fed mice. Fecal microbiota characterization by high throughput 16S rRNA gene sequencing revealed that a HFD increased the <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and LCBP reduced this ratio by increasing the relative abundance of <i>Bacteroides,</i> <i>Parabacteroides</i>, and another two undefined bacterial genera belonging to the order of <i>Bacteroidales</i> and family of <i>Rikenellaceae</i>, and also by decreasing the relative abundance of six bacterial genera belonging to the phylum <i>Firmicutes</i>, including <i>Staphylococcus</i>, <i>Lactobacillus</i>, <i>Ruminococcus</i>, and <i>Oscillospira</i>. These data demonstrated that LCBP potentially attenuated inflammation in NAFLD through modulation of gut microbiota, especially the ratio of <i>Firmicutes</i> to <i>Bacteroidetes</i>. |
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| language | English |
| last_indexed | 2024-04-13T10:02:43Z |
| publishDate | 2018-12-01 |
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| series | Molecules |
| spelling | doaj.art-2d1ece0a6b0e4713928eeda801c185962022-12-22T02:51:11ZengMDPI AGMolecules1420-30492018-12-012312321310.3390/molecules23123213molecules23123213Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat DietShusong Wu0Ruizhi Hu1Hironobu Nakano2Keyu Chen3Ming Liu4Xi He5Hongfu Zhang6Jianhua He7De-Xing Hou8Core Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaCore Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaThe United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, JapanThe United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, JapanCore Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaCore Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaThe State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, ChinaCore Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaCore Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, ChinaPolyphenols from the <i>Lonicera caerulea</i> L. berry have shown protective effects on experimental non-alcoholic fatty liver disease (NAFLD) in our previous studies. As endotoxins from gut bacteria are considered to be the major trigger of inflammation in NAFLD, this study aims to clarify the regulatory effects of <i>L. caerulea</i> L. berry polyphenols (LCBP) on gut microbiota in a high fat diet (HFD)-induced mouse model. C57BL/6N mice were fed with a normal diet, HFD, or HFD containing 0.5⁻1% of LCBP for 45 days. The results revealed that supplementation with LCBP decreased significantly the levels of IL-2, IL-6, MCP-1, and TNF-α in serum, as well as endotoxin levels in both serum and liver in HFD-fed mice. Fecal microbiota characterization by high throughput 16S rRNA gene sequencing revealed that a HFD increased the <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and LCBP reduced this ratio by increasing the relative abundance of <i>Bacteroides,</i> <i>Parabacteroides</i>, and another two undefined bacterial genera belonging to the order of <i>Bacteroidales</i> and family of <i>Rikenellaceae</i>, and also by decreasing the relative abundance of six bacterial genera belonging to the phylum <i>Firmicutes</i>, including <i>Staphylococcus</i>, <i>Lactobacillus</i>, <i>Ruminococcus</i>, and <i>Oscillospira</i>. These data demonstrated that LCBP potentially attenuated inflammation in NAFLD through modulation of gut microbiota, especially the ratio of <i>Firmicutes</i> to <i>Bacteroidetes</i>.https://www.mdpi.com/1420-3049/23/12/3213polyphenols<i>Lonicera caerulea</i> L.fatty liver diseaseinflammationgut microbiota |
| spellingShingle | Shusong Wu Ruizhi Hu Hironobu Nakano Keyu Chen Ming Liu Xi He Hongfu Zhang Jianhua He De-Xing Hou Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet Molecules polyphenols <i>Lonicera caerulea</i> L. fatty liver disease inflammation gut microbiota |
| title | Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet |
| title_full | Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet |
| title_fullStr | Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet |
| title_full_unstemmed | Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet |
| title_short | Modulation of Gut Microbiota by <i>Lonicera caerulea</i> L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet |
| title_sort | modulation of gut microbiota by i lonicera caerulea i l berry polyphenols in a mouse model of fatty liver induced by high fat diet |
| topic | polyphenols <i>Lonicera caerulea</i> L. fatty liver disease inflammation gut microbiota |
| url | https://www.mdpi.com/1420-3049/23/12/3213 |
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