Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease
Objective: To explore the effect of Sirt1 on the function of endothelial progenitor cells (EPCs) in rats with chronic obstructive pulmonary disease (COPD). Methods: A rat COPD model was established via smoking and endotoxin administration for three months. The peripheral circulating EPCs were isolat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=10;spage=429;epage=439;aulast=Sun |
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| author | Dong-Mei Sun Jin-Jian Yao Zhan-Ling Dong Jin Qian Qi-Feng Huang Yuan-Tian Sun Xiao-Ran Liu |
| author_facet | Dong-Mei Sun Jin-Jian Yao Zhan-Ling Dong Jin Qian Qi-Feng Huang Yuan-Tian Sun Xiao-Ran Liu |
| author_sort | Dong-Mei Sun |
| collection | DOAJ |
| description | Objective: To explore the effect of Sirt1 on the function of endothelial progenitor cells (EPCs) in rats with chronic obstructive pulmonary disease (COPD).
Methods: A rat COPD model was established via smoking and endotoxin administration for three months. The peripheral circulating EPCs were isolated by gradient centrifugation, and their functions, cell cycle distribution, apoptosis, and Sirt1 expression were examined. The function changes of EPCs in the presence or absence of Sirt1 agonist and inhibitor were estimated; meanwhile, the expressions of Sirt1, FOXO3a, NF-κB, and p53 were also evaluated.
Results: The proliferation, adhesion, and migration of EPCs decreased while the apoptosis rate was increased in the COPD rats. The expression of Sirt1 protein in EPCs of the COPD group was significantly lower than that in the control group (P<0.01). The overexpression of the Sirt1 gene using a gene transfection technique or Sirt1 agonists (SRT1720) improved the proliferation, migration, and adhesion, and decreased the apoptosis of EPC. However, Sirt1 inhibitor (EX527) decreased EPC functions in the COPD group. The effect of Sirt1 expression on EPC function may be related to reduction of FOXO3a and increase of NF-κB and p53 activity.
Conclusions: Increased expression of Sirt1 can improve the proliferation and migration of EPCs and reduce their apoptosis in COPD rats. This change may be related to FOXO3a, NF-κB, and p53 signaling pathways. |
| first_indexed | 2024-04-11T21:27:25Z |
| format | Article |
| id | doaj.art-2d1f29f0f8c74930a82622c14f917c43 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-04-11T21:27:25Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-2d1f29f0f8c74930a82622c14f917c432022-12-22T04:02:21ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222021-01-01111042943910.4103/2221-1691.326097Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary diseaseDong-Mei SunJin-Jian YaoZhan-Ling DongJin QianQi-Feng HuangYuan-Tian SunXiao-Ran LiuObjective: To explore the effect of Sirt1 on the function of endothelial progenitor cells (EPCs) in rats with chronic obstructive pulmonary disease (COPD). Methods: A rat COPD model was established via smoking and endotoxin administration for three months. The peripheral circulating EPCs were isolated by gradient centrifugation, and their functions, cell cycle distribution, apoptosis, and Sirt1 expression were examined. The function changes of EPCs in the presence or absence of Sirt1 agonist and inhibitor were estimated; meanwhile, the expressions of Sirt1, FOXO3a, NF-κB, and p53 were also evaluated. Results: The proliferation, adhesion, and migration of EPCs decreased while the apoptosis rate was increased in the COPD rats. The expression of Sirt1 protein in EPCs of the COPD group was significantly lower than that in the control group (P<0.01). The overexpression of the Sirt1 gene using a gene transfection technique or Sirt1 agonists (SRT1720) improved the proliferation, migration, and adhesion, and decreased the apoptosis of EPC. However, Sirt1 inhibitor (EX527) decreased EPC functions in the COPD group. The effect of Sirt1 expression on EPC function may be related to reduction of FOXO3a and increase of NF-κB and p53 activity. Conclusions: Increased expression of Sirt1 can improve the proliferation and migration of EPCs and reduce their apoptosis in COPD rats. This change may be related to FOXO3a, NF-κB, and p53 signaling pathways.http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=10;spage=429;epage=439;aulast=Sunchronic obstructive pulmonary disease; endothelial progenitor cells; sirt1; proliferation; migration; adhesion; apoptosis; foxo3a; nf-κb; p53; rat |
| spellingShingle | Dong-Mei Sun Jin-Jian Yao Zhan-Ling Dong Jin Qian Qi-Feng Huang Yuan-Tian Sun Xiao-Ran Liu Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease Asian Pacific Journal of Tropical Biomedicine chronic obstructive pulmonary disease; endothelial progenitor cells; sirt1; proliferation; migration; adhesion; apoptosis; foxo3a; nf-κb; p53; rat |
| title | Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease |
| title_full | Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease |
| title_fullStr | Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease |
| title_full_unstemmed | Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease |
| title_short | Effects of Sirt1 on proliferation, migration, and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease |
| title_sort | effects of sirt1 on proliferation migration and apoptosis of endothelial progenitor cells in peripheral blood of sd rats with chronic obstructive pulmonary disease |
| topic | chronic obstructive pulmonary disease; endothelial progenitor cells; sirt1; proliferation; migration; adhesion; apoptosis; foxo3a; nf-κb; p53; rat |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=10;spage=429;epage=439;aulast=Sun |
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