Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)

In order to standardize cellular hematology practices, the French-speaking Cellular Hematology Group (Groupe Francophone d’Hématologie Cellulaire, GFHC) focused on Perls’ stain. A national survey was carried out, leading to the proposal of recommendations on insoluble iron detection and quantificati...

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Main Authors: Camille Lours, Laurane Cottin, Margaux Wiber, Valérie Andrieu, Véronique Baccini, Lucile Baseggio, Chantal Brouzes, Bernard Chatelain, Sylvie Daliphard, Odile Fenneteau, Franck Geneviève, Sandrine Girard, Vincent Leymarie, Karim Maloum, Jean-Baptiste Rieu, Gérard Sebahoun, Isabelle Sudaka, Xavier Troussard, Orianne Wagner-Ballon, Soraya Wuilleme, Valérie Bardet, Jean-François Lesesve
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Diagnostics
Subjects:
Online Access:https://www.mdpi.com/2075-4418/12/7/1698
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author Camille Lours
Laurane Cottin
Margaux Wiber
Valérie Andrieu
Véronique Baccini
Lucile Baseggio
Chantal Brouzes
Bernard Chatelain
Sylvie Daliphard
Odile Fenneteau
Franck Geneviève
Sandrine Girard
Vincent Leymarie
Karim Maloum
Jean-Baptiste Rieu
Gérard Sebahoun
Isabelle Sudaka
Xavier Troussard
Orianne Wagner-Ballon
Soraya Wuilleme
Valérie Bardet
Jean-François Lesesve
author_facet Camille Lours
Laurane Cottin
Margaux Wiber
Valérie Andrieu
Véronique Baccini
Lucile Baseggio
Chantal Brouzes
Bernard Chatelain
Sylvie Daliphard
Odile Fenneteau
Franck Geneviève
Sandrine Girard
Vincent Leymarie
Karim Maloum
Jean-Baptiste Rieu
Gérard Sebahoun
Isabelle Sudaka
Xavier Troussard
Orianne Wagner-Ballon
Soraya Wuilleme
Valérie Bardet
Jean-François Lesesve
author_sort Camille Lours
collection DOAJ
description In order to standardize cellular hematology practices, the French-speaking Cellular Hematology Group (Groupe Francophone d’Hématologie Cellulaire, GFHC) focused on Perls’ stain. A national survey was carried out, leading to the proposal of recommendations on insoluble iron detection and quantification in bone marrow. The criteria presented here met with a “strong professional agreement” and follow the suggestions of the World Health Organization’s classification of hematological malignancies.
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spelling doaj.art-2d1faa5853434345ab5e7f264167be432023-12-03T14:55:05ZengMDPI AGDiagnostics2075-44182022-07-01127169810.3390/diagnostics12071698Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)Camille Lours0Laurane Cottin1Margaux Wiber2Valérie Andrieu3Véronique Baccini4Lucile Baseggio5Chantal Brouzes6Bernard Chatelain7Sylvie Daliphard8Odile Fenneteau9Franck Geneviève10Sandrine Girard11Vincent Leymarie12Karim Maloum13Jean-Baptiste Rieu14Gérard Sebahoun15Isabelle Sudaka16Xavier Troussard17Orianne Wagner-Ballon18Soraya Wuilleme19Valérie Bardet20Jean-François Lesesve21Service d’Hématologie Biologique, Centre de Biologie et de Pathologie Est, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, FranceLaboratoire d’Hématologie, Centre Hospitalier Universitaire d’Angers, 49100 Angers, FranceLaboratoire d’Hématologie, Centre Hospitalier Universitaire d’Angers, 49100 Angers, FranceDépartement d’Hématologie et Immunologie, Groupement Hospitalier Bichat Claude Bernard, 75018 Paris, FranceLaboratoire d’Hématologie Immunologie, Centre Hospitalier Universitaire de Pointe-à-Pitre, 97159 Pointe-à-Pitre, FranceService d’Hématologie Biologique, Centre de Biologie et de Pathologie Sud, Hôpital Lyon Sud, Hospices Civils de Lyon, 69495 Pierre Bénite, FranceService d’Hématologie Biologique, Hôpital Necker, 75015 Paris, FranceLaboratoire d’Hématologie, Clinique Universitaire UCL de Mont-Godinne, 5530 Yvoir, BelgiumLaboratoire d’Hématologie, Centre Hospitalier Universitaire de Rouen, 76000 Rouen, FranceLaboratoire d’Hématologie, Hôpital Robert Debré, 75019 Paris, FranceLaboratoire d’Hématologie, Centre Hospitalier Universitaire d’Angers, 49100 Angers, FranceService d’Hématologie Biologique, Centre de Biologie et de Pathologie Est, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, FranceLaboratoire d’Hématologie, Centre Hospitalier d’Avicenne, 93000 Bobigny, FranceService d’Hématologie Biologique, Hôpital Pitié Salpêtrière, 75013 Paris, FranceLaboratoire d’Hématologie, Institut Universitaire du Cancer—Oncopôle, 31100 Toulouse, FranceLaboratoire d’Hématologie, APHM Hôpital Nord, 13005 Marseille, FranceLaboratoire d’Hématologie, Hôpital Pasteur, 06000 Nice, FranceLaboratoire d’Hématologie, Centre Hospitalier Universitaire de Caen, 14033 Caen, FranceDépartement d’Hématologie et d’Immunologie Biologiques, Hôpital Universitaire Henri Mondor, 94000 Créteil, FranceLaboratoire d’Hématologie, Centre Hospitalier de Nantes, 44000 Nantes, FranceService d’Hématologie-Immunologie-Transfusion, CHU Ambroise Paré, INSERM UMR 1184, AP-HP, Université Paris Saclay, 92100 Boulogne Billancourt, FranceLaboratoire d’Hématologie, Centre Hospitalier Universitaire de Nancy, 54000 Nancy, FranceIn order to standardize cellular hematology practices, the French-speaking Cellular Hematology Group (Groupe Francophone d’Hématologie Cellulaire, GFHC) focused on Perls’ stain. A national survey was carried out, leading to the proposal of recommendations on insoluble iron detection and quantification in bone marrow. The criteria presented here met with a “strong professional agreement” and follow the suggestions of the World Health Organization’s classification of hematological malignancies.https://www.mdpi.com/2075-4418/12/7/1698myelodysplastic syndromesPerls’ stainPrussian blue staincytochemistryrecommendationsbone marrow
spellingShingle Camille Lours
Laurane Cottin
Margaux Wiber
Valérie Andrieu
Véronique Baccini
Lucile Baseggio
Chantal Brouzes
Bernard Chatelain
Sylvie Daliphard
Odile Fenneteau
Franck Geneviève
Sandrine Girard
Vincent Leymarie
Karim Maloum
Jean-Baptiste Rieu
Gérard Sebahoun
Isabelle Sudaka
Xavier Troussard
Orianne Wagner-Ballon
Soraya Wuilleme
Valérie Bardet
Jean-François Lesesve
Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
Diagnostics
myelodysplastic syndromes
Perls’ stain
Prussian blue stain
cytochemistry
recommendations
bone marrow
title Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
title_full Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
title_fullStr Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
title_full_unstemmed Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
title_short Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)
title_sort perls stain guidelines from the french speaking cellular hematology group gfhc
topic myelodysplastic syndromes
Perls’ stain
Prussian blue stain
cytochemistry
recommendations
bone marrow
url https://www.mdpi.com/2075-4418/12/7/1698
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