Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71
The three human Enterovirus serotypes D-68, D-70, and A-71, are common pathogens that are transmitted by fecal-oral and aerosol routes. These positive RNA viruses were known to exhibit high levels of genetic diversity and variability. Currently, no vaccines are available to protect humans from these...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2021-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/computational-approach-for-predicting-common-epitopes-in-the-vp1-structural-protein-of-enterovirus-serotypes-ev-d68-ev-d70-and-ev-a71/ |
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| author | Rihabe Boussettine Yassine Kasmi Najwa Hassou Hlima Bessi Moulay Mustapha Ennaji |
| author_facet | Rihabe Boussettine Yassine Kasmi Najwa Hassou Hlima Bessi Moulay Mustapha Ennaji |
| author_sort | Rihabe Boussettine |
| collection | DOAJ |
| description | The three human Enterovirus serotypes D-68, D-70, and A-71, are common pathogens that are transmitted by fecal-oral and aerosol routes. These positive RNA viruses were known to exhibit high levels of genetic diversity and variability. Currently, no vaccines are available to protect humans from these three serotypes. Therefore, efforts are needed for the development of a vaccine directed against heterologous viruses. In our study, an immunoinformatics approach is used to identify T- and B-cell epitopes that may help for the generation of a universal vaccine against EV-D70, EV-A71, and EV-D68. B and T cell epitopes were selected based on their length. As a result, 5 B cell epitopes and 18 T cell epitopes were predicted. Our B cell epitope prediction results showed that there are a number of linear regions. Position 150-170 was found to be the most immunogenic for the different strains. Regarding the epitopes of the T lymphocytes, the result of the interactions shows that 95% of the predicted epitopes are common between the 3 sequences and the 5 methods used. These results demonstrate the great immunogenic potential of these sequences and their capacities to trigger immune reactions in people with different HLA alleles. The “VFYDGFAGF” epitope is the most important and most immunogenic for triggering an immune response. Our study results allowed us to identify epitopes to be used in the development of cross-protection vaccines against the three Enterovirus serotypes. However, in vivo and in vitro studies are needed to assess the potential of the epitopes predicted by our study. |
| first_indexed | 2024-12-17T15:27:18Z |
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| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T15:27:18Z |
| publishDate | 2021-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-2d22adad0f3e45ff85625c951f8aaf8f2022-12-21T21:43:15ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-12-011541855186310.22207/JPAM.15.4.06Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71Rihabe Boussettinehttps://orcid.org/0000-0001-9324-7712Yassine Kasmihttps://orcid.org/0000-0001-9592-7906Najwa Hassouhttps://orcid.org/0000-0002-9295-3735Hlima BessiMoulay Mustapha Ennajihttps://orcid.org/0000-0001-5809-0270The three human Enterovirus serotypes D-68, D-70, and A-71, are common pathogens that are transmitted by fecal-oral and aerosol routes. These positive RNA viruses were known to exhibit high levels of genetic diversity and variability. Currently, no vaccines are available to protect humans from these three serotypes. Therefore, efforts are needed for the development of a vaccine directed against heterologous viruses. In our study, an immunoinformatics approach is used to identify T- and B-cell epitopes that may help for the generation of a universal vaccine against EV-D70, EV-A71, and EV-D68. B and T cell epitopes were selected based on their length. As a result, 5 B cell epitopes and 18 T cell epitopes were predicted. Our B cell epitope prediction results showed that there are a number of linear regions. Position 150-170 was found to be the most immunogenic for the different strains. Regarding the epitopes of the T lymphocytes, the result of the interactions shows that 95% of the predicted epitopes are common between the 3 sequences and the 5 methods used. These results demonstrate the great immunogenic potential of these sequences and their capacities to trigger immune reactions in people with different HLA alleles. The “VFYDGFAGF” epitope is the most important and most immunogenic for triggering an immune response. Our study results allowed us to identify epitopes to be used in the development of cross-protection vaccines against the three Enterovirus serotypes. However, in vivo and in vitro studies are needed to assess the potential of the epitopes predicted by our study.https://microbiologyjournal.org/computational-approach-for-predicting-common-epitopes-in-the-vp1-structural-protein-of-enterovirus-serotypes-ev-d68-ev-d70-and-ev-a71/enterovirusepitopesvp1 structural proteincomputational prediction |
| spellingShingle | Rihabe Boussettine Yassine Kasmi Najwa Hassou Hlima Bessi Moulay Mustapha Ennaji Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 Journal of Pure and Applied Microbiology enterovirus epitopes vp1 structural protein computational prediction |
| title | Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 |
| title_full | Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 |
| title_fullStr | Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 |
| title_full_unstemmed | Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 |
| title_short | Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71 |
| title_sort | computational approach for predicting common epitopes in the vp1 structural protein of enterovirus serotypes ev d68 ev d70 and ev a71 |
| topic | enterovirus epitopes vp1 structural protein computational prediction |
| url | https://microbiologyjournal.org/computational-approach-for-predicting-common-epitopes-in-the-vp1-structural-protein-of-enterovirus-serotypes-ev-d68-ev-d70-and-ev-a71/ |
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