The role of malate in the synthesis of glutamate in Pisum arvense roots
The in vivo and in vitro activities of NADH-dependent glutamate synthase in excised Pisum arvense roots increased several-fold under the influence of malate while pyruvate oxaloacctate. citrate and succinate inhibited this entyme. The plastids isolated from Pisum arvense root,. ahen incubated with g...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3037 |
| _version_ | 1819047918172110848 |
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| author | Genowefa Kubik-Dorosz |
| author_facet | Genowefa Kubik-Dorosz |
| author_sort | Genowefa Kubik-Dorosz |
| collection | DOAJ |
| description | The in vivo and in vitro activities of NADH-dependent glutamate synthase in excised Pisum arvense roots increased several-fold under the influence of malate while pyruvate oxaloacctate. citrate and succinate inhibited this entyme. The plastids isolated from Pisum arvense root,. ahen incubated with glutamine and α-ketoglutarate, released glutamate into the medium Malate clearly stimulated this process. Albizziin (25 mM) completely reduced the presence of glutamate in the incubation mixture. These results indicate that reduced pyridine nucleotides arising in P. arvense root plastids during oxidation of malic acid may constitute the indispensable source of electrons for glutamic acid synthesis. |
| first_indexed | 2024-12-21T11:07:59Z |
| format | Article |
| id | doaj.art-2d26747f84804655b48b6ef0927469bf |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-21T11:07:59Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-2d26747f84804655b48b6ef0927469bf2022-12-21T19:06:10ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-0155461161910.5586/asbp.1986.0502515The role of malate in the synthesis of glutamate in Pisum arvense rootsGenowefa Kubik-Dorosz0Wrocław UniversityThe in vivo and in vitro activities of NADH-dependent glutamate synthase in excised Pisum arvense roots increased several-fold under the influence of malate while pyruvate oxaloacctate. citrate and succinate inhibited this entyme. The plastids isolated from Pisum arvense root,. ahen incubated with glutamine and α-ketoglutarate, released glutamate into the medium Malate clearly stimulated this process. Albizziin (25 mM) completely reduced the presence of glutamate in the incubation mixture. These results indicate that reduced pyridine nucleotides arising in P. arvense root plastids during oxidation of malic acid may constitute the indispensable source of electrons for glutamic acid synthesis.https://pbsociety.org.pl/journals/index.php/asbp/article/view/3037rootsNADH-glutamatesynthasemalate |
| spellingShingle | Genowefa Kubik-Dorosz The role of malate in the synthesis of glutamate in Pisum arvense roots Acta Societatis Botanicorum Poloniae roots NADH-glutamate synthase malate |
| title | The role of malate in the synthesis of glutamate in Pisum arvense roots |
| title_full | The role of malate in the synthesis of glutamate in Pisum arvense roots |
| title_fullStr | The role of malate in the synthesis of glutamate in Pisum arvense roots |
| title_full_unstemmed | The role of malate in the synthesis of glutamate in Pisum arvense roots |
| title_short | The role of malate in the synthesis of glutamate in Pisum arvense roots |
| title_sort | role of malate in the synthesis of glutamate in pisum arvense roots |
| topic | roots NADH-glutamate synthase malate |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3037 |
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