In Vivo Detection of Metabolic Fluctuations in Real Time Using the NanoBiT Technology Based on PII Signalling Protein Interactions

In Vivo Detection of Metabolic Fluctuations in Real Time Using the NanoBiT Technology Based on PII Signalling Protein Interactions

New protein-fragment complementation assays (PCA) have successfully been developed to characterize protein–protein interactions in vitro and in vivo. Notably, the NanoBiT technology, employing fragment complementation of NanoLuc luciferase, stands out for its high sensitivity, wide dynamic range, an...

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Bibliographic Details
Main Authors: Rokhsareh Rozbeh, Karl Forchhammer
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:International Journal of Molecular Sciences
Subjects:
protein-fragment complementation assay
NanoLuc
luciferase
2-oxoglutarate
PII-interacting protein X (PipX)
N-Acetylglutamate kinase (NAGK)
Online Access:https://www.mdpi.com/1422-0067/25/6/3409

Internet

https://www.mdpi.com/1422-0067/25/6/3409

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