The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome
Aim:The mesocorticolimbic dopaminergic system, especially the nucleus accumbens, is an important region in opioid dependence and withdrawal. Studies have shown that nitric oxide synthase inhibitors modulate the development of tolerance to opioids, opioid dependence, and withdrawal. In this study, we...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2020-04-01
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| Series: | Namık Kemal Tıp Dergisi |
| Subjects: | |
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http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effects-of-l-name-and-agmatine-in-the-nucleus-/40344
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| author | Mahluga JAFAROVA DEMİRKAPU Hasan Raci YANANLI Elmar MAMMADOV Ina DERVİSHİ Ali KIRBAŞ Şafak Recep YAŞAR Tzemal SALİ Mansur KURBANOĞLU Merve ÇAĞLAR Öykü USLU Halil Eren SAKALLİ Betilay TOPKARA |
| author_facet | Mahluga JAFAROVA DEMİRKAPU Hasan Raci YANANLI Elmar MAMMADOV Ina DERVİSHİ Ali KIRBAŞ Şafak Recep YAŞAR Tzemal SALİ Mansur KURBANOĞLU Merve ÇAĞLAR Öykü USLU Halil Eren SAKALLİ Betilay TOPKARA |
| author_sort | Mahluga JAFAROVA DEMİRKAPU |
| collection | DOAJ |
| description | Aim:The mesocorticolimbic dopaminergic system, especially the nucleus accumbens, is an important region in opioid dependence and withdrawal. Studies have shown that nitric oxide synthase inhibitors modulate the development of tolerance to opioids, opioid dependence, and withdrawal. In this study, we aimed to investigate the effects of local injections of L-NAME and agmatine into the nucleus accumbens core (NAcc), one of the nucleus accumbens subregions on withdrawal signs and locomotor activity behavior during naloxone-induced withdrawal in morphine-dependent rats.Materials and Methods:Twenty-four adult Sprague-Dawley rats were used in the study. Morphine dependence was developed in all animals after guide cannula implantation into the NAcc region. On the last day of experiment, following bilateral L-NAME, agmatine or artificial cerebrospinal fluid (aCSF, control group) microinjections morphine withdrawal was induced by naloxone.Results:Local administration of agmatine and L-NAME into the NAcc significantly suppressed the jumping number during naloxone induced withdrawal. Local agmatine treatment significantly suppressed the score of teeth chattering, although the L-NAME did not change. No significant difference was observed in withdrawal symptoms such as wet dog shakes and defecation after local agmatine and L-NAME treatment. Agmatine increased stereotypic movements, but did not change locomotor activity behaviors such as ambulatory activity and total covered distance. Local administration of L-NAME into the NAcc did not increase stereotypic and ambulatory movements, and total covered distance during naloxone-induced withdrawal.Conclusion:These results suggest that inhibition of nitric oxide synthesis in NAcc plays a role in morphine withdrawal symptoms, but it is not responsible alone. |
| first_indexed | 2024-04-10T14:10:45Z |
| format | Article |
| id | doaj.art-2d28af3214984a908d61f097c5092689 |
| institution | Directory Open Access Journal |
| issn | 2587-0262 |
| language | English |
| last_indexed | 2024-04-10T14:10:45Z |
| publishDate | 2020-04-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Namık Kemal Tıp Dergisi |
| spelling | doaj.art-2d28af3214984a908d61f097c50926892023-02-15T16:09:43ZengGalenos YayincilikNamık Kemal Tıp Dergisi2587-02622020-04-0181657210.37696/nkmj.65454213049054The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal SyndromeMahluga JAFAROVA DEMİRKAPU0Hasan Raci YANANLI1Elmar MAMMADOV2Ina DERVİSHİ3Ali KIRBAŞ4Şafak Recep YAŞAR5Tzemal SALİ6Mansur KURBANOĞLU7Merve ÇAĞLAR8Öykü USLU9Halil Eren SAKALLİ10Betilay TOPKARA11 Tekirdag Namik Kemal University, Department of Pharmacology, Tekirdag, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Marmara University School of Medicine, Istanbul, TURKEY Eastern Mediterranean University Faculty of Medicine, Famagusta, NORTH CYPRUS Koç University School of Medicine, Istanbul, TURKEY Aim:The mesocorticolimbic dopaminergic system, especially the nucleus accumbens, is an important region in opioid dependence and withdrawal. Studies have shown that nitric oxide synthase inhibitors modulate the development of tolerance to opioids, opioid dependence, and withdrawal. In this study, we aimed to investigate the effects of local injections of L-NAME and agmatine into the nucleus accumbens core (NAcc), one of the nucleus accumbens subregions on withdrawal signs and locomotor activity behavior during naloxone-induced withdrawal in morphine-dependent rats.Materials and Methods:Twenty-four adult Sprague-Dawley rats were used in the study. Morphine dependence was developed in all animals after guide cannula implantation into the NAcc region. On the last day of experiment, following bilateral L-NAME, agmatine or artificial cerebrospinal fluid (aCSF, control group) microinjections morphine withdrawal was induced by naloxone.Results:Local administration of agmatine and L-NAME into the NAcc significantly suppressed the jumping number during naloxone induced withdrawal. Local agmatine treatment significantly suppressed the score of teeth chattering, although the L-NAME did not change. No significant difference was observed in withdrawal symptoms such as wet dog shakes and defecation after local agmatine and L-NAME treatment. Agmatine increased stereotypic movements, but did not change locomotor activity behaviors such as ambulatory activity and total covered distance. Local administration of L-NAME into the NAcc did not increase stereotypic and ambulatory movements, and total covered distance during naloxone-induced withdrawal.Conclusion:These results suggest that inhibition of nitric oxide synthesis in NAcc plays a role in morphine withdrawal symptoms, but it is not responsible alone. http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effects-of-l-name-and-agmatine-in-the-nucleus-/40344 nucleus accumbensl-nameagmatinemorphinewithdrawal |
| spellingShingle | Mahluga JAFAROVA DEMİRKAPU Hasan Raci YANANLI Elmar MAMMADOV Ina DERVİSHİ Ali KIRBAŞ Şafak Recep YAŞAR Tzemal SALİ Mansur KURBANOĞLU Merve ÇAĞLAR Öykü USLU Halil Eren SAKALLİ Betilay TOPKARA The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome Namık Kemal Tıp Dergisi nucleus accumbens l-name agmatine morphine withdrawal |
| title | The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome |
| title_full | The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome |
| title_fullStr | The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome |
| title_full_unstemmed | The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome |
| title_short | The Effects of L-NAME and Agmatine in The Nucleus Accumbens Core Regıon on Morphine Withdrawal Syndrome |
| title_sort | effects of l name and agmatine in the nucleus accumbens core region on morphine withdrawal syndrome |
| topic | nucleus accumbens l-name agmatine morphine withdrawal |
| url |
http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effects-of-l-name-and-agmatine-in-the-nucleus-/40344
|
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