A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses
Summary: Recently, traces of zoonotic viruses have been discovered in bats and other species around the world, but despite repeated attempts, full viral genomes have not been rescued. The absence of critical genetic sequences from these viruses and the difficulties to isolate infectious virus from s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718312622 |
| _version_ | 1818919709291053056 |
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| author | Whitney A. Manhart Jennifer R. Pacheco Adam J. Hume Tessa N. Cressey Laure R. Deflubé Elke Mühlberger |
| author_facet | Whitney A. Manhart Jennifer R. Pacheco Adam J. Hume Tessa N. Cressey Laure R. Deflubé Elke Mühlberger |
| author_sort | Whitney A. Manhart |
| collection | DOAJ |
| description | Summary: Recently, traces of zoonotic viruses have been discovered in bats and other species around the world, but despite repeated attempts, full viral genomes have not been rescued. The absence of critical genetic sequences from these viruses and the difficulties to isolate infectious virus from specimens prevent research on their pathogenic potential for humans. One example of these zoonotic pathogens is Lloviu virus (LLOV), a filovirus that is closely related to Ebola virus. Here, we established LLOV minigenome systems based on sequence complementation from other filoviruses. Our results show that the LLOV replication and transcription mechanisms are, in general, more similar to ebolaviruses than to marburgviruses. We also show that a single nucleotide at the 3′ genome end determines species specificity of the LLOV polymerase. The data obtained here will be instrumental for the rescue of infectious LLOV clones for pathogenesis studies. : Lloviu virus (LLOV) is a filovirus of unknown pathogenicity, and the viral genome ends have not been recovered. Manhart et al. established a minigenome system for studying LLOV by complementing missing sequence information with that of other filoviruses. This minigenome provides a blueprint for generating recombinant LLOV for pathogenesis studies. Keywords: Lloviu virus, filoviruses, Ebola virus, Marburg virus, minigenomes, replication and transcription, RNA-dependent RNA polymerase, emerging viruses |
| first_indexed | 2024-12-20T01:10:10Z |
| format | Article |
| id | doaj.art-2d2a22a40624401c8dd05c0962db95cb |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T01:10:10Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-2d2a22a40624401c8dd05c0962db95cb2022-12-21T19:58:42ZengElsevierCell Reports2211-12472018-09-01241025732580.e4A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than MarburgvirusesWhitney A. Manhart0Jennifer R. Pacheco1Adam J. Hume2Tessa N. Cressey3Laure R. Deflubé4Elke Mühlberger5Department of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USADepartment of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USADepartment of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USADepartment of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USADepartment of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USADepartment of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, MA 02118, USA; Corresponding authorSummary: Recently, traces of zoonotic viruses have been discovered in bats and other species around the world, but despite repeated attempts, full viral genomes have not been rescued. The absence of critical genetic sequences from these viruses and the difficulties to isolate infectious virus from specimens prevent research on their pathogenic potential for humans. One example of these zoonotic pathogens is Lloviu virus (LLOV), a filovirus that is closely related to Ebola virus. Here, we established LLOV minigenome systems based on sequence complementation from other filoviruses. Our results show that the LLOV replication and transcription mechanisms are, in general, more similar to ebolaviruses than to marburgviruses. We also show that a single nucleotide at the 3′ genome end determines species specificity of the LLOV polymerase. The data obtained here will be instrumental for the rescue of infectious LLOV clones for pathogenesis studies. : Lloviu virus (LLOV) is a filovirus of unknown pathogenicity, and the viral genome ends have not been recovered. Manhart et al. established a minigenome system for studying LLOV by complementing missing sequence information with that of other filoviruses. This minigenome provides a blueprint for generating recombinant LLOV for pathogenesis studies. Keywords: Lloviu virus, filoviruses, Ebola virus, Marburg virus, minigenomes, replication and transcription, RNA-dependent RNA polymerase, emerging viruseshttp://www.sciencedirect.com/science/article/pii/S2211124718312622 |
| spellingShingle | Whitney A. Manhart Jennifer R. Pacheco Adam J. Hume Tessa N. Cressey Laure R. Deflubé Elke Mühlberger A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses Cell Reports |
| title | A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses |
| title_full | A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses |
| title_fullStr | A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses |
| title_full_unstemmed | A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses |
| title_short | A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses |
| title_sort | chimeric lloviu virus minigenome system reveals that the bat derived filovirus replicates more similarly to ebolaviruses than marburgviruses |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718312622 |
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