Comprehensive analysis of bacteriocins in Streptococcus mutans
Abstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Ba...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-92370-1 |
| _version_ | 1818719121676697600 |
|---|---|
| author | Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa |
| author_facet | Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa |
| author_sort | Atsuko Watanabe |
| collection | DOAJ |
| description | Abstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques. |
| first_indexed | 2024-12-17T20:01:55Z |
| format | Article |
| id | doaj.art-2d2bc72b0c464574a17e78b72286db91 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T20:01:55Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2d2bc72b0c464574a17e78b72286db912022-12-21T21:34:27ZengNature PortfolioScientific Reports2045-23222021-06-0111111310.1038/s41598-021-92370-1Comprehensive analysis of bacteriocins in Streptococcus mutansAtsuko Watanabe0Miki Kawada-Matsuo1Mi Nguyen-Tra Le2Junzo Hisatsune3Yuichi Oogai4Yoshio Nakano5Masanobu Nakata6Shouichi Miyawaki7Motoyuki Sugai8Hitoshi Komatsuzawa9Department of Orthodontics and Dentofacial Orthopedics, Kagoshima University Graduate School of Medical and Dental SciencesDepartment of Bacteriology, Hiroshima University Graduate School of Biomedical and Health SciencesDepartment of Bacteriology, Hiroshima University Graduate School of Biomedical and Health SciencesProject Research Centre for Nosocomial Infectious Diseases, Hiroshima UniversityDepartment of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental SciencesDepartment of Chemistry, Nihon University School of DentistryDepartment of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental SciencesDepartment of Orthodontics and Dentofacial Orthopedics, Kagoshima University Graduate School of Medical and Dental SciencesProject Research Centre for Nosocomial Infectious Diseases, Hiroshima UniversityDepartment of Bacteriology, Hiroshima University Graduate School of Biomedical and Health SciencesAbstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques.https://doi.org/10.1038/s41598-021-92370-1 |
| spellingShingle | Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa Comprehensive analysis of bacteriocins in Streptococcus mutans Scientific Reports |
| title | Comprehensive analysis of bacteriocins in Streptococcus mutans |
| title_full | Comprehensive analysis of bacteriocins in Streptococcus mutans |
| title_fullStr | Comprehensive analysis of bacteriocins in Streptococcus mutans |
| title_full_unstemmed | Comprehensive analysis of bacteriocins in Streptococcus mutans |
| title_short | Comprehensive analysis of bacteriocins in Streptococcus mutans |
| title_sort | comprehensive analysis of bacteriocins in streptococcus mutans |
| url | https://doi.org/10.1038/s41598-021-92370-1 |
| work_keys_str_mv | AT atsukowatanabe comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT mikikawadamatsuo comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT minguyentrale comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT junzohisatsune comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT yuichioogai comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT yoshionakano comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT masanobunakata comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT shouichimiyawaki comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT motoyukisugai comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT hitoshikomatsuzawa comprehensiveanalysisofbacteriocinsinstreptococcusmutans |