Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury
Abstract Background Acute traumatic cervical spinal cord injury (SCI) is a leading cause of disability in adolescents and young adults worldwide. Evidence from previous studies suggests that circulating cell-free DNA is associated with severity following acute injury. The present study determined wh...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12967-019-2084-z |
| _version_ | 1818453764538892288 |
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| author | Hung-Chen Wang Yu-Tsai Lin Shih-Yuan Hsu Nai-Wen Tsai Yun-Ru Lai Ben Yu-Jih Su Chia-Te Kung Cheng-Hsien Lu |
| author_facet | Hung-Chen Wang Yu-Tsai Lin Shih-Yuan Hsu Nai-Wen Tsai Yun-Ru Lai Ben Yu-Jih Su Chia-Te Kung Cheng-Hsien Lu |
| author_sort | Hung-Chen Wang |
| collection | DOAJ |
| description | Abstract Background Acute traumatic cervical spinal cord injury (SCI) is a leading cause of disability in adolescents and young adults worldwide. Evidence from previous studies suggests that circulating cell-free DNA is associated with severity following acute injury. The present study determined whether plasma DNA levels in acute cervical SCI are predictive of outcome. Methods In present study, serial plasma nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) levels were obtained from 44 patients with acute traumatic cervical SCI at five time points from day 1 to day 180 post-injury. Control blood samples were obtained from 66 volunteers. Results Data showed a significant increase in plasma nDNA and mtDNA concentrations at admission in SCI patients compared to the control group. Plasma nDNA levels at admission, but not plasma mtDNA levels, were significantly associated with the Japanese Orthopaedic Association (JOA) score and Injury Severity Score in patients with acute traumatic cervical SCI. In patients with non-excellent outcomes, plasma nDNA increased significantly at days 1, 14 and 30 post-injury. Furthermore, its level at day 14 was independently associated with outcome. Higher plasma nDNA levels at the chosen cutoff point (> 45.6 ng/ml) predicted poorer outcome with a sensitivity of 78.9% and a specificity of 78.4%. Conclusions These results indicate JOA score performance and plasma nDNA levels reflect the severity of spinal cord injury. Therefore, the plasma nDNA assays can be considered as potential neuropathological markers in patients with acute traumatic cervical SCI. |
| first_indexed | 2024-12-14T21:44:10Z |
| format | Article |
| id | doaj.art-2d2d202151604d3d9ceab5ba2cfb0c0f |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-14T21:44:10Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-2d2d202151604d3d9ceab5ba2cfb0c0f2022-12-21T22:46:23ZengBMCJournal of Translational Medicine1479-58762019-10-0117111110.1186/s12967-019-2084-zSerial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injuryHung-Chen Wang0Yu-Tsai Lin1Shih-Yuan Hsu2Nai-Wen Tsai3Yun-Ru Lai4Ben Yu-Jih Su5Chia-Te Kung6Cheng-Hsien Lu7Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Neurology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Neurology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Emergency Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Neurology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineAbstract Background Acute traumatic cervical spinal cord injury (SCI) is a leading cause of disability in adolescents and young adults worldwide. Evidence from previous studies suggests that circulating cell-free DNA is associated with severity following acute injury. The present study determined whether plasma DNA levels in acute cervical SCI are predictive of outcome. Methods In present study, serial plasma nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) levels were obtained from 44 patients with acute traumatic cervical SCI at five time points from day 1 to day 180 post-injury. Control blood samples were obtained from 66 volunteers. Results Data showed a significant increase in plasma nDNA and mtDNA concentrations at admission in SCI patients compared to the control group. Plasma nDNA levels at admission, but not plasma mtDNA levels, were significantly associated with the Japanese Orthopaedic Association (JOA) score and Injury Severity Score in patients with acute traumatic cervical SCI. In patients with non-excellent outcomes, plasma nDNA increased significantly at days 1, 14 and 30 post-injury. Furthermore, its level at day 14 was independently associated with outcome. Higher plasma nDNA levels at the chosen cutoff point (> 45.6 ng/ml) predicted poorer outcome with a sensitivity of 78.9% and a specificity of 78.4%. Conclusions These results indicate JOA score performance and plasma nDNA levels reflect the severity of spinal cord injury. Therefore, the plasma nDNA assays can be considered as potential neuropathological markers in patients with acute traumatic cervical SCI.http://link.springer.com/article/10.1186/s12967-019-2084-zTraumatic spinal cord injuryPlasma DNAOutcomeJapanese Orthopaedic Association score |
| spellingShingle | Hung-Chen Wang Yu-Tsai Lin Shih-Yuan Hsu Nai-Wen Tsai Yun-Ru Lai Ben Yu-Jih Su Chia-Te Kung Cheng-Hsien Lu Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury Journal of Translational Medicine Traumatic spinal cord injury Plasma DNA Outcome Japanese Orthopaedic Association score |
| title | Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| title_full | Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| title_fullStr | Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| title_full_unstemmed | Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| title_short | Serial plasma DNA levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| title_sort | serial plasma dna levels as predictors of outcome in patients with acute traumatic cervical spinal cord injury |
| topic | Traumatic spinal cord injury Plasma DNA Outcome Japanese Orthopaedic Association score |
| url | http://link.springer.com/article/10.1186/s12967-019-2084-z |
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