Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong
<i>Staphylococcus argenteus</i> is a novel Staphylococcus species derived from <i>Staphylococcus aureus</i>. Information on the prevalence and genetic characteristics of invasive <i>S. argenteus</i> in Asia is limited. In this study, 275 invasive <i>S. aureu...
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2435 |
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| author | Jonathan H. K. Chen Hoi-Yi Leung Charles M. C. Wong Kwok-Yung Yuen Vincent C. C. Cheng |
| author_facet | Jonathan H. K. Chen Hoi-Yi Leung Charles M. C. Wong Kwok-Yung Yuen Vincent C. C. Cheng |
| author_sort | Jonathan H. K. Chen |
| collection | DOAJ |
| description | <i>Staphylococcus argenteus</i> is a novel Staphylococcus species derived from <i>Staphylococcus aureus</i>. Information on the prevalence and genetic characteristics of invasive <i>S. argenteus</i> in Asia is limited. In this study, 275 invasive <i>S. aureus</i> complex strains were retrieved from blood culture specimens in Hong Kong and re-analyzed using MALDI-TOF mass spectrometry and an in-house multiplex real-time PCR for <i>S. argenteus</i>. The prevalence of invasive <i>S. argenteus</i> in Hong Kong was found to be 4.0% (11/275). These strains were primarily susceptible to commonly used antibiotics, except penicillin. Whole-genome sequencing revealed the circulation of three <i>S. argenteus</i> genotypes (ST-2250, ST-1223, and ST-2854) in Hong Kong, with ST-2250 and ST-1223 being the predominant genotypes. The local ST-2250 and ST-1223 strains showed close phylogenetic relationships with isolates from mainland China. Antimicrobial-resistant genes (<i>fosB</i>, <i>tet-38</i>, <i>mepA</i>, <i>blaI</i>, <i>blaZ</i>) could be found in nearly all local <i>S. argenteus</i> strains. The ST-1223 and ST-2250 genotypes carried multiple staphylococcal enterotoxin genes that could cause food poisoning and toxic shock syndrome. The CRISPR/Cas locus was observed only in the ST-2250 strains. This study provides the first report on the molecular epidemiology of invasive <i>S. argenteus</i> in Hong Kong, and further analysis is needed to understand its transmission reservoir. |
| first_indexed | 2024-03-10T21:02:22Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T21:02:22Z |
| publishDate | 2023-09-01 |
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| series | Microorganisms |
| spelling | doaj.art-2d3151215b1040768883bb1a241e836c2023-11-19T17:26:42ZengMDPI AGMicroorganisms2076-26072023-09-011110243510.3390/microorganisms11102435Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong KongJonathan H. K. Chen0Hoi-Yi Leung1Charles M. C. Wong2Kwok-Yung Yuen3Vincent C. C. Cheng4Department of Microbiology, Queen Mary Hospital, Hong Kong SAR, ChinaDepartment of Microbiology, Queen Mary Hospital, Hong Kong SAR, ChinaDepartment of Microbiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong SAR, ChinaDepartment of Microbiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong SAR, ChinaDepartment of Microbiology, Queen Mary Hospital, Hong Kong SAR, China<i>Staphylococcus argenteus</i> is a novel Staphylococcus species derived from <i>Staphylococcus aureus</i>. Information on the prevalence and genetic characteristics of invasive <i>S. argenteus</i> in Asia is limited. In this study, 275 invasive <i>S. aureus</i> complex strains were retrieved from blood culture specimens in Hong Kong and re-analyzed using MALDI-TOF mass spectrometry and an in-house multiplex real-time PCR for <i>S. argenteus</i>. The prevalence of invasive <i>S. argenteus</i> in Hong Kong was found to be 4.0% (11/275). These strains were primarily susceptible to commonly used antibiotics, except penicillin. Whole-genome sequencing revealed the circulation of three <i>S. argenteus</i> genotypes (ST-2250, ST-1223, and ST-2854) in Hong Kong, with ST-2250 and ST-1223 being the predominant genotypes. The local ST-2250 and ST-1223 strains showed close phylogenetic relationships with isolates from mainland China. Antimicrobial-resistant genes (<i>fosB</i>, <i>tet-38</i>, <i>mepA</i>, <i>blaI</i>, <i>blaZ</i>) could be found in nearly all local <i>S. argenteus</i> strains. The ST-1223 and ST-2250 genotypes carried multiple staphylococcal enterotoxin genes that could cause food poisoning and toxic shock syndrome. The CRISPR/Cas locus was observed only in the ST-2250 strains. This study provides the first report on the molecular epidemiology of invasive <i>S. argenteus</i> in Hong Kong, and further analysis is needed to understand its transmission reservoir.https://www.mdpi.com/2076-2607/11/10/2435<i>Staphylococcus argenteus</i><i>Staphylococcus aureus</i>bacteremiaprevalencewhole-genome sequencingHong Kong |
| spellingShingle | Jonathan H. K. Chen Hoi-Yi Leung Charles M. C. Wong Kwok-Yung Yuen Vincent C. C. Cheng Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong Microorganisms <i>Staphylococcus argenteus</i> <i>Staphylococcus aureus</i> bacteremia prevalence whole-genome sequencing Hong Kong |
| title | Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong |
| title_full | Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong |
| title_fullStr | Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong |
| title_full_unstemmed | Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong |
| title_short | Prevalence and Characteristics of Invasive <i>Staphylococcus argenteus</i> among Patients with Bacteremia in Hong Kong |
| title_sort | prevalence and characteristics of invasive i staphylococcus argenteus i among patients with bacteremia in hong kong |
| topic | <i>Staphylococcus argenteus</i> <i>Staphylococcus aureus</i> bacteremia prevalence whole-genome sequencing Hong Kong |
| url | https://www.mdpi.com/2076-2607/11/10/2435 |
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