Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface
Dental ceramic material is one of the widely preferred restorative materials to mimic the natural tooth enamel surface. However, it has continuously been degraded because of low wear resistance during mastication in the oral cavity. The friction involved was reduced by introducing the lubricant sali...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/8/1658 |
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| author | Nareshkumar Baskaran You-Cheng Chang Chia-Hua Chang Shun-Kai Hung Chuan-Tse Kao Yang Wei |
| author_facet | Nareshkumar Baskaran You-Cheng Chang Chia-Hua Chang Shun-Kai Hung Chuan-Tse Kao Yang Wei |
| author_sort | Nareshkumar Baskaran |
| collection | DOAJ |
| description | Dental ceramic material is one of the widely preferred restorative materials to mimic the natural tooth enamel surface. However, it has continuously been degraded because of low wear resistance during mastication in the oral cavity. The friction involved was reduced by introducing the lubricant saliva protein layers to improve the wear resistance of the dental materials. However, little is understood regarding how the protein–protein interactions (PPI) influence the adsorbed-state structures and lubricating behaviors of saliva proteins on the ceramic material surface. The objective of this study is to quantify the influences of PPI effects on the structural changes and corresponding oral lubrications of adsorbed α-amylase, one of the abundant proteins in the saliva, on the dental ceramic material with glass as a model surface. α-Amylase was first adsorbed to glass surface under varying protein solution concentrations to saturate the surface to vary the PPI effects over a wide range. The areal density of the adsorbed protein was measured as an indicator of the level of PPI effects within the layer, and these values were then correlated with the measurements of the adsorbed protein’s secondary structure and corresponding friction coefficient. The decreased friction coefficient value was an indicator of the lubricated surfaces with higher wear resistance. Our results indicate that PPI effects help stabilize the structure of α-amylase adsorbed on glass, and the correlation observed between the friction coefficient and the conformational state of adsorbed α-amylase was apparent. This study thus provides new molecular-level insights into how PPI influences the structure and lubricating behaviors of adsorbed protein, which is critical for the innovations of dental ceramic material designs with improved wear resistance. |
| first_indexed | 2024-03-10T18:12:48Z |
| format | Article |
| id | doaj.art-2d330e28899c47f697cfb3da96f7d569 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T18:12:48Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-2d330e28899c47f697cfb3da96f7d5692023-11-20T07:57:42ZengMDPI AGPolymers2073-43602020-07-01128165810.3390/polym12081658Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass SurfaceNareshkumar Baskaran0You-Cheng Chang1Chia-Hua Chang2Shun-Kai Hung3Chuan-Tse Kao4Yang Wei5Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Section 3, Zhongxiao East Road, Taipei 10608, TaiwanDental ceramic material is one of the widely preferred restorative materials to mimic the natural tooth enamel surface. However, it has continuously been degraded because of low wear resistance during mastication in the oral cavity. The friction involved was reduced by introducing the lubricant saliva protein layers to improve the wear resistance of the dental materials. However, little is understood regarding how the protein–protein interactions (PPI) influence the adsorbed-state structures and lubricating behaviors of saliva proteins on the ceramic material surface. The objective of this study is to quantify the influences of PPI effects on the structural changes and corresponding oral lubrications of adsorbed α-amylase, one of the abundant proteins in the saliva, on the dental ceramic material with glass as a model surface. α-Amylase was first adsorbed to glass surface under varying protein solution concentrations to saturate the surface to vary the PPI effects over a wide range. The areal density of the adsorbed protein was measured as an indicator of the level of PPI effects within the layer, and these values were then correlated with the measurements of the adsorbed protein’s secondary structure and corresponding friction coefficient. The decreased friction coefficient value was an indicator of the lubricated surfaces with higher wear resistance. Our results indicate that PPI effects help stabilize the structure of α-amylase adsorbed on glass, and the correlation observed between the friction coefficient and the conformational state of adsorbed α-amylase was apparent. This study thus provides new molecular-level insights into how PPI influences the structure and lubricating behaviors of adsorbed protein, which is critical for the innovations of dental ceramic material designs with improved wear resistance.https://www.mdpi.com/2073-4360/12/8/1658dental ceramic materialglass surfaceα-amylaseprotein–protein interactionsconformational changesfriction coefficient |
| spellingShingle | Nareshkumar Baskaran You-Cheng Chang Chia-Hua Chang Shun-Kai Hung Chuan-Tse Kao Yang Wei Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface Polymers dental ceramic material glass surface α-amylase protein–protein interactions conformational changes friction coefficient |
| title | Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface |
| title_full | Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface |
| title_fullStr | Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface |
| title_full_unstemmed | Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface |
| title_short | Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface |
| title_sort | quantify the protein protein interaction effects on adsorption related lubricating behaviors of α amylase on a glass surface |
| topic | dental ceramic material glass surface α-amylase protein–protein interactions conformational changes friction coefficient |
| url | https://www.mdpi.com/2073-4360/12/8/1658 |
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