THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE
Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2019-06-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/842 |
| _version_ | 1797394190057865216 |
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| author | Marzena Parzymies Krystyna Pudelska Monika Poniewozik |
| author_facet | Marzena Parzymies Krystyna Pudelska Monika Poniewozik |
| author_sort | Marzena Parzymies |
| collection | DOAJ |
| description |
Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm Ag–3 NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
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| first_indexed | 2024-03-09T00:15:52Z |
| format | Article |
| id | doaj.art-2d3315089fbf4f77b3f5e13287209ec3 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:15:52Z |
| publishDate | 2019-06-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-2d3315089fbf4f77b3f5e13287209ec32023-12-12T07:54:21ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052019-06-0118310.24326/asphc.2019.3.12THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTUREMarzena Parzymies0Krystyna Pudelska1Monika Poniewozik2Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, PolandDepartment of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, PolandDepartment of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm Ag–3 NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/842nano-particlesculture initiationmicropropagationplant growth regulatorscontaminationsregeneration |
| spellingShingle | Marzena Parzymies Krystyna Pudelska Monika Poniewozik THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE Acta Scientiarum Polonorum: Hortorum Cultus nano-particles culture initiation micropropagation plant growth regulators contaminations regeneration |
| title | THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE |
| title_full | THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE |
| title_fullStr | THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE |
| title_full_unstemmed | THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE |
| title_short | THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE |
| title_sort | use of nano silver for disinfection of pennisetum alopecuroides plant material for tissue culture |
| topic | nano-particles culture initiation micropropagation plant growth regulators contaminations regeneration |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/842 |
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