Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics
Developmental processes are governed by a diverse suite of signaling pathways employing reversible phosphorylation. Recent advances in large-scale phosphoproteomic methodologies have made possible the identification and quantification of hundreds to thousands of phosphorylation sites from primary ti...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-04-01
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| Series: | Proteomes |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2227-7382/2/2/191 |
| _version_ | 1798042173097115648 |
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| author | Peter F. Doubleday Bryan A. Ballif |
| author_facet | Peter F. Doubleday Bryan A. Ballif |
| author_sort | Peter F. Doubleday |
| collection | DOAJ |
| description | Developmental processes are governed by a diverse suite of signaling pathways employing reversible phosphorylation. Recent advances in large-scale phosphoproteomic methodologies have made possible the identification and quantification of hundreds to thousands of phosphorylation sites from primary tissues. Towards a global characterization of proteomic changes across brain development, we present the results of a large-scale quantitative mass spectrometry study comparing embryonic, newborn and adult murine brain. Using anti-phosphotyrosine immuno-affinity chromatography and strong cation exchange (SCX) chromatography, coupled to immobilized metal affinity chromatography (IMAC), we identified and quantified over 1,750 phosphorylation sites and over 1,300 proteins between three developmental states. Bioinformatic analyses highlight functions associated with the identified proteins and phosphoproteins and their enrichment at distinct developmental stages. These results serve as a primary reference resource and reveal dynamic developmental profiles of proteins and phosphoproteins from the developing murine brain. |
| first_indexed | 2024-04-11T22:31:48Z |
| format | Article |
| id | doaj.art-2d331b29d36e4b0ab4f7773d6da56494 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T22:31:48Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-2d331b29d36e4b0ab4f7773d6da564942022-12-22T03:59:22ZengMDPI AGProteomes2227-73822014-04-012219120710.3390/proteomes2020191proteomes2020191Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative ProteomicsPeter F. Doubleday0Bryan A. Ballif1Department of Biology, University of Vermont, 109 Carrigan Drive, Burlington, VT 05405, USADepartment of Biology, University of Vermont, 109 Carrigan Drive, Burlington, VT 05405, USADevelopmental processes are governed by a diverse suite of signaling pathways employing reversible phosphorylation. Recent advances in large-scale phosphoproteomic methodologies have made possible the identification and quantification of hundreds to thousands of phosphorylation sites from primary tissues. Towards a global characterization of proteomic changes across brain development, we present the results of a large-scale quantitative mass spectrometry study comparing embryonic, newborn and adult murine brain. Using anti-phosphotyrosine immuno-affinity chromatography and strong cation exchange (SCX) chromatography, coupled to immobilized metal affinity chromatography (IMAC), we identified and quantified over 1,750 phosphorylation sites and over 1,300 proteins between three developmental states. Bioinformatic analyses highlight functions associated with the identified proteins and phosphoproteins and their enrichment at distinct developmental stages. These results serve as a primary reference resource and reveal dynamic developmental profiles of proteins and phosphoproteins from the developing murine brain.http://www.mdpi.com/2227-7382/2/2/191brain developmentphosphoproteomicsphosphorylationquantitative mass spectrometryreductive amination |
| spellingShingle | Peter F. Doubleday Bryan A. Ballif Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics Proteomes brain development phosphoproteomics phosphorylation quantitative mass spectrometry reductive amination |
| title | Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics |
| title_full | Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics |
| title_fullStr | Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics |
| title_full_unstemmed | Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics |
| title_short | Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics |
| title_sort | developmentally dynamic murine brain proteomes and phosphoproteomes revealed by quantitative proteomics |
| topic | brain development phosphoproteomics phosphorylation quantitative mass spectrometry reductive amination |
| url | http://www.mdpi.com/2227-7382/2/2/191 |
| work_keys_str_mv | AT peterfdoubleday developmentallydynamicmurinebrainproteomesandphosphoproteomesrevealedbyquantitativeproteomics AT bryanaballif developmentallydynamicmurinebrainproteomesandphosphoproteomesrevealedbyquantitativeproteomics |