In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte.
Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of os...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-07-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2705681?pdf=render |
| _version_ | 1818966129356308480 |
|---|---|
| author | Musa M Mhlanga Diana P Bratu Auguste Genovesio Agata Rybarska Nicolas Chenouard Ulf Nehrbass Jean-Christophe Olivo-Marin |
| author_facet | Musa M Mhlanga Diana P Bratu Auguste Genovesio Agata Rybarska Nicolas Chenouard Ulf Nehrbass Jean-Christophe Olivo-Marin |
| author_sort | Musa M Mhlanga |
| collection | DOAJ |
| description | Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport. oskar mRNA stably associated with Staufen near the posterior pole. We observe oskar mRNA to oligomerize as hundreds of copies forming large particles which are necessary for its long range transport and localization. We show the formation of these particles occurs in the nurse cell nucleus in an Hrp48-dependent manner. We present a more refined model of oskar mRNA transport in the Drosophila oocyte. |
| first_indexed | 2024-12-20T13:27:59Z |
| format | Article |
| id | doaj.art-2d34d70f6c89490ab99d68d8dc5143e8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T13:27:59Z |
| publishDate | 2009-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2d34d70f6c89490ab99d68d8dc5143e82022-12-21T19:39:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-07-0147e624110.1371/journal.pone.0006241In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte.Musa M MhlangaDiana P BratuAuguste GenovesioAgata RybarskaNicolas ChenouardUlf NehrbassJean-Christophe Olivo-MarinEfficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport. oskar mRNA stably associated with Staufen near the posterior pole. We observe oskar mRNA to oligomerize as hundreds of copies forming large particles which are necessary for its long range transport and localization. We show the formation of these particles occurs in the nurse cell nucleus in an Hrp48-dependent manner. We present a more refined model of oskar mRNA transport in the Drosophila oocyte.http://europepmc.org/articles/PMC2705681?pdf=render |
| spellingShingle | Musa M Mhlanga Diana P Bratu Auguste Genovesio Agata Rybarska Nicolas Chenouard Ulf Nehrbass Jean-Christophe Olivo-Marin In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. PLoS ONE |
| title | In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. |
| title_full | In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. |
| title_fullStr | In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. |
| title_full_unstemmed | In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. |
| title_short | In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte. |
| title_sort | in vivo colocalisation of oskar mrna and trans acting proteins revealed by quantitative imaging of the drosophila oocyte |
| url | http://europepmc.org/articles/PMC2705681?pdf=render |
| work_keys_str_mv | AT musammhlanga invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT dianapbratu invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT augustegenovesio invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT agatarybarska invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT nicolaschenouard invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT ulfnehrbass invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte AT jeanchristopheolivomarin invivocolocalisationofoskarmrnaandtransactingproteinsrevealedbyquantitativeimagingofthedrosophilaoocyte |