Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a
High plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-depende...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2016-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606&lng=en&tlng=en |
| _version_ | 1811259580408135680 |
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| author | M. Fang A. Jin Y. Zhao X. Liu |
| author_facet | M. Fang A. Jin Y. Zhao X. Liu |
| author_sort | M. Fang |
| collection | DOAJ |
| description | High plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-dependent manner, and apoptosis was induced within 48 h. The median effective concentration (EC50) of Hcy was approximately 5 mM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear translocation and acylation has been implicated in the regulation of apoptosis. We found that nuclear translocation and acetylation of GAPDH increased in the presence of 5 mM Hcy and that higher levels of acetyltransferase p300/CBP were detected in Neuro2a cells. These findings implicate the involvement of GAPDH in the mechanism whereby Hcy induces apoptosis in neurons. This study highlights a potentially important pathway in neurodegenerative disorders, and a novel target pathway for neuroprotective therapy. |
| first_indexed | 2024-04-12T18:33:47Z |
| format | Article |
| id | doaj.art-2d35bcc5a0bd414f8f346dbb89074c7e |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-12T18:33:47Z |
| publishDate | 2016-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-2d35bcc5a0bd414f8f346dbb89074c7e2022-12-22T03:20:59ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2016-01-0149210.1590/1414-431X20154543S0100-879X2016000200606Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2aM. FangA. JinY. ZhaoX. LiuHigh plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-dependent manner, and apoptosis was induced within 48 h. The median effective concentration (EC50) of Hcy was approximately 5 mM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear translocation and acylation has been implicated in the regulation of apoptosis. We found that nuclear translocation and acetylation of GAPDH increased in the presence of 5 mM Hcy and that higher levels of acetyltransferase p300/CBP were detected in Neuro2a cells. These findings implicate the involvement of GAPDH in the mechanism whereby Hcy induces apoptosis in neurons. This study highlights a potentially important pathway in neurodegenerative disorders, and a novel target pathway for neuroprotective therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606&lng=en&tlng=enHomocysteineGlyceraldehyde-3-phosphate dehydrogenaseApoptosisAcetylatep300/CBP |
| spellingShingle | M. Fang A. Jin Y. Zhao X. Liu Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a Brazilian Journal of Medical and Biological Research Homocysteine Glyceraldehyde-3-phosphate dehydrogenase Apoptosis Acetylate p300/CBP |
| title | Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a |
| title_full | Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a |
| title_fullStr | Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a |
| title_full_unstemmed | Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a |
| title_short | Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a |
| title_sort | homocysteine induces glyceraldehyde 3 phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line neuro2a |
| topic | Homocysteine Glyceraldehyde-3-phosphate dehydrogenase Apoptosis Acetylate p300/CBP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606&lng=en&tlng=en |
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