Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-09-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.1515/acve-2017-0028 |
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| author | Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana |
| author_facet | Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana |
| author_sort | Bjelica Jelena |
| collection | DOAJ |
| description | Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU- group; selenium adequate, PTU+ group; selenium defi cient, PTU- group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T4), triiodothyronine (T3), and thyroid stimulating hormone (TSH) were determined. The animals having “intermediate” concentrations of T3 (1.56-1.69 nmol/L) and T4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T3≤1.55 nmol/L; T4≤40 nmol/L), while PTU- groups included euthyroid rats (T3≥1.70 nmol/L; T4≥50 nmol/L). Both groups of selenium deficient rats had, when compared to the control group, a significantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium deficiency nor PTU influenced the adherence of peritoneal macrophages. Selenium deficiency significantly decreased the peroxide synthesis in macrophages and significantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium deficiency had no influence on these parameters. |
| first_indexed | 2024-04-11T20:24:40Z |
| format | Article |
| id | doaj.art-2d3898b6cb864e669bb26e9095d8a275 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-04-11T20:24:40Z |
| publishDate | 2017-09-01 |
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| series | Acta Veterinaria |
| spelling | doaj.art-2d3898b6cb864e669bb26e9095d8a2752022-12-22T04:04:41ZengSciendoActa Veterinaria1820-74482017-09-0167334035510.1515/acve-2017-0028Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile RatsBjelica Jelena0Jovanović Ivan B.1Ilić Vesna2Valčić Olivera3Kirovski Danijela4Gvozdić Dragan5Milanović Svetlana6Medicine and Medical Devices Agency of Serbia, Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaInstitute for Medical Research, University of Belgrade, Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaDepartment of Pathophysioplogy, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaBoth selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU- group; selenium adequate, PTU+ group; selenium defi cient, PTU- group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T4), triiodothyronine (T3), and thyroid stimulating hormone (TSH) were determined. The animals having “intermediate” concentrations of T3 (1.56-1.69 nmol/L) and T4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T3≤1.55 nmol/L; T4≤40 nmol/L), while PTU- groups included euthyroid rats (T3≥1.70 nmol/L; T4≥50 nmol/L). Both groups of selenium deficient rats had, when compared to the control group, a significantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium deficiency nor PTU influenced the adherence of peritoneal macrophages. Selenium deficiency significantly decreased the peroxide synthesis in macrophages and significantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium deficiency had no influence on these parameters.https://doi.org/10.1515/acve-2017-0028glutathione peroxidaseigmmacrophage adherencemacrophage peroxide productionratsseleniumthyroid hormones |
| spellingShingle | Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats Acta Veterinaria glutathione peroxidase igm macrophage adherence macrophage peroxide production rats selenium thyroid hormones |
| title | Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats |
| title_full | Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats |
| title_fullStr | Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats |
| title_full_unstemmed | Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats |
| title_short | Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats |
| title_sort | effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural igm antibodies in juvenile rats |
| topic | glutathione peroxidase igm macrophage adherence macrophage peroxide production rats selenium thyroid hormones |
| url | https://doi.org/10.1515/acve-2017-0028 |
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