Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species
Grape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2023-11-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202304-0007_detection-and-molecular-identification-of-8216-candidatus-phytoplasma-asteris-8217-associated-with-muscari.php |
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| author | Emese Mergenthaler József Fodor János Ágoston Orsolya Viczián |
| author_facet | Emese Mergenthaler József Fodor János Ágoston Orsolya Viczián |
| author_sort | Emese Mergenthaler |
| collection | DOAJ |
| description | Grape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of leaves. Symptomatic and asymptomatic Muscari plants were sampled at two locations to detect and identify the pathogens involved. Total DNA was extracted from the plants and used as a template in polymerase chain reaction assays to amplify 16S rRNA gene sequences and housekeeping genes (tuf, secY) with phytoplasma-specific primers. The resulting PCR products from symptomatic plants were cloned and sequenced bidirectionally. Homology searching of the obtained sequences against the GenBank database indicated the presence of 'Candidatus Phytoplasma asteris' in the three Muscari species. This is the first report worldwide of C. P. asteris phytoplasma infecting M. botryoides and M. comosum, and its first identification in M. armeniacum in Hungary. |
| first_indexed | 2024-03-09T14:19:26Z |
| format | Article |
| id | doaj.art-2d38a029fb4c4431aa0b5a7f6afe6dc2 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-03-09T14:19:26Z |
| publishDate | 2023-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-2d38a029fb4c4431aa0b5a7f6afe6dc22023-11-28T15:46:58ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412023-11-0159438939310.17221/70/2023-PPSpps-202304-0007Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth speciesEmese Mergenthaler0József Fodor1János Ágoston2Orsolya Viczián3Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, HungaryPlant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, HungaryELKH-SZE PhatoPlant-Lab, Széchenyi István University, Mosonmagyaróvár, HungaryPlant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, HungaryGrape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of leaves. Symptomatic and asymptomatic Muscari plants were sampled at two locations to detect and identify the pathogens involved. Total DNA was extracted from the plants and used as a template in polymerase chain reaction assays to amplify 16S rRNA gene sequences and housekeeping genes (tuf, secY) with phytoplasma-specific primers. The resulting PCR products from symptomatic plants were cloned and sequenced bidirectionally. Homology searching of the obtained sequences against the GenBank database indicated the presence of 'Candidatus Phytoplasma asteris' in the three Muscari species. This is the first report worldwide of C. P. asteris phytoplasma infecting M. botryoides and M. comosum, and its first identification in M. armeniacum in Hungary.https://pps.agriculturejournals.cz/artkey/pps-202304-0007_detection-and-molecular-identification-of-8216-candidatus-phytoplasma-asteris-8217-associated-with-muscari.phpgrape hyacinthsphytoplasmavirescenceaster yellows |
| spellingShingle | Emese Mergenthaler József Fodor János Ágoston Orsolya Viczián Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species Plant Protection Science grape hyacinths phytoplasma virescence aster yellows |
| title | Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species |
| title_full | Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species |
| title_fullStr | Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species |
| title_full_unstemmed | Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species |
| title_short | Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species |
| title_sort | detection and molecular identification of candidatus phytoplasma asteris associated with muscari virescence of three grape hyacinth species |
| topic | grape hyacinths phytoplasma virescence aster yellows |
| url | https://pps.agriculturejournals.cz/artkey/pps-202304-0007_detection-and-molecular-identification-of-8216-candidatus-phytoplasma-asteris-8217-associated-with-muscari.php |
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