Effects of Three Commonly-used Diuretics on the Urinary Proteome
Biomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to keep the internal environment homeostatic, urine is more likely to reflect changes of the body. As a result, urine is likely to be a better biomarker source than blo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | Genomics, Proteomics & Bioinformatics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022914000059 |
| _version_ | 1797368011782356992 |
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| author | Xundou Li Mindi Zhao Menglin Li Lulu Jia Youhe Gao |
| author_facet | Xundou Li Mindi Zhao Menglin Li Lulu Jia Youhe Gao |
| author_sort | Xundou Li |
| collection | DOAJ |
| description | Biomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to keep the internal environment homeostatic, urine is more likely to reflect changes of the body. As a result, urine is likely to be a better biomarker source than blood. However, since the urinary proteome is affected by many factors, including diuretics, careful evaluation of those effects is necessary if urinary proteomics is used for biomarker discovery. Here, we evaluated the effects of three commonly-used diuretics (furosemide, F; hydrochlorothiazide, H; and spirolactone, S) on the urinary proteome in rats. Urine samples were collected before and after intragastric administration of diuretics at therapeutic doses and the proteomes were analyzed using label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS). Based on the criteria of P ⩽ 0.05, a fold change ⩾2, a spectral count ⩾5, and false positive rate (FDR) ⩽1%, 14 proteins (seven for F, five for H, and two for S) were identified by Progenesis LC–MS. The human orthologs of most of these 14 proteins are stable in the healthy human urinary proteome, and ten of them are reported as disease biomarkers. Thus, our results suggest that the effects of diuretics deserve more attention in future urinary protein biomarker studies. Moreover, the distinct effects of diuretics on the urinary proteome may provide clues to the mechanisms of diuretics. |
| first_indexed | 2024-03-08T17:25:25Z |
| format | Article |
| id | doaj.art-2d38cf791cf142cb87043d58f75e95a6 |
| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2024-03-08T17:25:25Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-2d38cf791cf142cb87043d58f75e95a62024-01-02T19:25:14ZengElsevierGenomics, Proteomics & Bioinformatics1672-02292014-06-0112312012610.1016/j.gpb.2013.12.002Effects of Three Commonly-used Diuretics on the Urinary ProteomeXundou LiMindi ZhaoMenglin LiLulu JiaYouhe GaoBiomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to keep the internal environment homeostatic, urine is more likely to reflect changes of the body. As a result, urine is likely to be a better biomarker source than blood. However, since the urinary proteome is affected by many factors, including diuretics, careful evaluation of those effects is necessary if urinary proteomics is used for biomarker discovery. Here, we evaluated the effects of three commonly-used diuretics (furosemide, F; hydrochlorothiazide, H; and spirolactone, S) on the urinary proteome in rats. Urine samples were collected before and after intragastric administration of diuretics at therapeutic doses and the proteomes were analyzed using label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS). Based on the criteria of P ⩽ 0.05, a fold change ⩾2, a spectral count ⩾5, and false positive rate (FDR) ⩽1%, 14 proteins (seven for F, five for H, and two for S) were identified by Progenesis LC–MS. The human orthologs of most of these 14 proteins are stable in the healthy human urinary proteome, and ten of them are reported as disease biomarkers. Thus, our results suggest that the effects of diuretics deserve more attention in future urinary protein biomarker studies. Moreover, the distinct effects of diuretics on the urinary proteome may provide clues to the mechanisms of diuretics.http://www.sciencedirect.com/science/article/pii/S1672022914000059Urinary proteomeDiureticsBiomarkers |
| spellingShingle | Xundou Li Mindi Zhao Menglin Li Lulu Jia Youhe Gao Effects of Three Commonly-used Diuretics on the Urinary Proteome Genomics, Proteomics & Bioinformatics Urinary proteome Diuretics Biomarkers |
| title | Effects of Three Commonly-used Diuretics on the Urinary Proteome |
| title_full | Effects of Three Commonly-used Diuretics on the Urinary Proteome |
| title_fullStr | Effects of Three Commonly-used Diuretics on the Urinary Proteome |
| title_full_unstemmed | Effects of Three Commonly-used Diuretics on the Urinary Proteome |
| title_short | Effects of Three Commonly-used Diuretics on the Urinary Proteome |
| title_sort | effects of three commonly used diuretics on the urinary proteome |
| topic | Urinary proteome Diuretics Biomarkers |
| url | http://www.sciencedirect.com/science/article/pii/S1672022914000059 |
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