Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss
Objective: The underlying mechanisms of polycystic ovarian syndrome (PCOS) are not fully understood yet. The aim of the study was to get functional insights into the regulation of steroid hormones in PCOS by steroid metabolomics. Design: This is a longitudinal study of changes of steroid hormones i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2017-05-01
|
| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | http://www.endocrineconnections.com/content/6/4/213.full |
| _version_ | 1818893243929067520 |
|---|---|
| author | Thomas Reinehr Alexandra Kulle Juliane Rothermel Caroline Knop-Schmenn Nina Lass Christina Bosse Paul-Martin Holterhus |
| author_facet | Thomas Reinehr Alexandra Kulle Juliane Rothermel Caroline Knop-Schmenn Nina Lass Christina Bosse Paul-Martin Holterhus |
| author_sort | Thomas Reinehr |
| collection | DOAJ |
| description | Objective: The underlying mechanisms of polycystic ovarian syndrome (PCOS) are not fully understood yet. The aim of the study was to get functional insights into the regulation of steroid hormones in PCOS by steroid metabolomics.
Design: This is a longitudinal study of changes of steroid hormones in 40 obese girls aged 13–16 years (50% with PCOS) participating in a 1-year lifestyle intervention. Girls with and without PCOS were matched to age, BMI and change of weight status.
Methods: We measured progesterone, 17-hydroxyprogesterone, 17-hydroxyprogenolon, 11-deoxycorticosterone, 21-deoxycorticosterone, deoxycorticosterone, corticosterone, 11-deoxycortisol, cortisol, cortisone, androstenedione, testosterone, dehydroepiandrostendione-sulfate (DHEA-S), estrone and estradiol by LC–MS/MS steroid profiling at baseline and one year later.
Results: At baseline, obese PCOS girls demonstrated significantly higher androstenedione and testosterone concentrations compared to obese girls without PCOS, whereas the other steroid hormones including glucocorticoids, mineralocorticoids, estrogens and precursors of androgens did not differ significantly. Weight loss in obese PCOS girls was associated with a significant decrease of testosterone, androstenedione, DHEA-S, cortisol and corticosterone concentrations. Weight loss in obese non-PCOS girls was associated with a significant decrease of DHEA-S, cortisol and corticosterone concentrations, whereas no significant changes of testosterone and androstenedione concentrations could be observed. Without weight loss, no significant changes of steroid hormones were measured except an increase of estradiol in obese PCOS girls without weight loss.
Conclusions: The key steroid hormones in obese adolescents with PCOS are androstenedione and testosterone, whereas glucocorticoids, mineralocorticoids, estrogens and precursors of androgens did not differ between obese girls with and without PCOS. |
| first_indexed | 2024-12-19T18:09:31Z |
| format | Article |
| id | doaj.art-2d3ba5be1b634eeaaa2736804cf61772 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-12-19T18:09:31Z |
| publishDate | 2017-05-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-2d3ba5be1b634eeaaa2736804cf617722022-12-21T20:11:21ZengBioscientificaEndocrine Connections2049-36142049-36142017-05-016421322410.1530/EC-17-0051Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight lossThomas Reinehr0Alexandra Kulle1Juliane Rothermel2Caroline Knop-Schmenn3Nina Lass4Christina Bosse5Paul-Martin Holterhus6Department of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Datteln, GermanyDivision of Pediatric Endocrinology and Diabetes, Department of Pediatrics, University Hospital of Schleswig – Holstein, UKSH, Campus Kiel/Christian Albrechts University of Kiel, CAU, Kiel, GermanyDepartment of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Datteln, GermanyDepartment of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Datteln, GermanyDepartment of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Datteln, GermanyDepartment of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Datteln, GermanyDivision of Pediatric Endocrinology and Diabetes, Department of Pediatrics, University Hospital of Schleswig – Holstein, UKSH, Campus Kiel/Christian Albrechts University of Kiel, CAU, Kiel, GermanyObjective: The underlying mechanisms of polycystic ovarian syndrome (PCOS) are not fully understood yet. The aim of the study was to get functional insights into the regulation of steroid hormones in PCOS by steroid metabolomics. Design: This is a longitudinal study of changes of steroid hormones in 40 obese girls aged 13–16 years (50% with PCOS) participating in a 1-year lifestyle intervention. Girls with and without PCOS were matched to age, BMI and change of weight status. Methods: We measured progesterone, 17-hydroxyprogesterone, 17-hydroxyprogenolon, 11-deoxycorticosterone, 21-deoxycorticosterone, deoxycorticosterone, corticosterone, 11-deoxycortisol, cortisol, cortisone, androstenedione, testosterone, dehydroepiandrostendione-sulfate (DHEA-S), estrone and estradiol by LC–MS/MS steroid profiling at baseline and one year later. Results: At baseline, obese PCOS girls demonstrated significantly higher androstenedione and testosterone concentrations compared to obese girls without PCOS, whereas the other steroid hormones including glucocorticoids, mineralocorticoids, estrogens and precursors of androgens did not differ significantly. Weight loss in obese PCOS girls was associated with a significant decrease of testosterone, androstenedione, DHEA-S, cortisol and corticosterone concentrations. Weight loss in obese non-PCOS girls was associated with a significant decrease of DHEA-S, cortisol and corticosterone concentrations, whereas no significant changes of testosterone and androstenedione concentrations could be observed. Without weight loss, no significant changes of steroid hormones were measured except an increase of estradiol in obese PCOS girls without weight loss. Conclusions: The key steroid hormones in obese adolescents with PCOS are androstenedione and testosterone, whereas glucocorticoids, mineralocorticoids, estrogens and precursors of androgens did not differ between obese girls with and without PCOS.http://www.endocrineconnections.com/content/6/4/213.fullandrogensglucocorticoidsmineralocorticoidspolycystic ovarian syndromelifestyle interventionobesity |
| spellingShingle | Thomas Reinehr Alexandra Kulle Juliane Rothermel Caroline Knop-Schmenn Nina Lass Christina Bosse Paul-Martin Holterhus Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss Endocrine Connections androgens glucocorticoids mineralocorticoids polycystic ovarian syndrome lifestyle intervention obesity |
| title | Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss |
| title_full | Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss |
| title_fullStr | Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss |
| title_full_unstemmed | Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss |
| title_short | Longitudinal analyses of the steroid metabolome in obese PCOS girls with weight loss |
| title_sort | longitudinal analyses of the steroid metabolome in obese pcos girls with weight loss |
| topic | androgens glucocorticoids mineralocorticoids polycystic ovarian syndrome lifestyle intervention obesity |
| url | http://www.endocrineconnections.com/content/6/4/213.full |
| work_keys_str_mv | AT thomasreinehr longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT alexandrakulle longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT julianerothermel longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT carolineknopschmenn longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT ninalass longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT christinabosse longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss AT paulmartinholterhus longitudinalanalysesofthesteroidmetabolomeinobesepcosgirlswithweightloss |