Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice
Acetate is associated with adipocyte differentiation and lipid deposition. To further develop this scientific point, obese mice on a high-fat diet were given an intragastric administration of acetate for 8 weeks and mouse adipose mesenchymal stem cells (mAMSCs) were treated with acetate for 24 h. Th...
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MDPI AG
2023-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/17/3736 |
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| author | Changbao Sun Ang Li Huan Wang Jiage Ma Juncai Hou |
| author_facet | Changbao Sun Ang Li Huan Wang Jiage Ma Juncai Hou |
| author_sort | Changbao Sun |
| collection | DOAJ |
| description | Acetate is associated with adipocyte differentiation and lipid deposition. To further develop this scientific point, obese mice on a high-fat diet were given an intragastric administration of acetate for 8 weeks and mouse adipose mesenchymal stem cells (mAMSCs) were treated with acetate for 24 h. The results showed that the body weight, food intake, Lee’s index, adipose tissue coefficient, liver index, blood lipid levels, insulin resistance, pro-inflammatory factors levels and fatty lesions in liver and adipose tissue in obese mice treated with acetate increased markedly, while anti-inflammatory factors levels and liver function decreased significantly (<i>p</i> < 0.05). The mRNA expression levels of PPAR-γ, C/EBP-α, SREBP, AFABP, FAS, ACC-1, SCD-1, LPL, LEPR, GPR41 and GPR43 genes in adipose tissue and mAMSCs were significantly increased, while the mRNA expression levels of HSL, CPT-1, CPT-2, AMPK, AdipoR1 and AdipoR2 genes were significantly reduced (<i>p</i> < 0.05). Except for AMPK-α signaling pathway proteins, the phosphorylation levels of p38 MAPK, ERK1/2, JNK and mTOR were significantly increased (<i>p</i> < 0.05) and these changes were dose-dependent. The findings indicated that acetate played a positive role in regulating adipocyte differentiation and lipid deposition by activating MAPKs and mTOR signaling pathways (the expression up-regulation of genes such as PPAR-γ, C/EBP-α and SREBP-1, etc.) and inhibiting the AMPK signaling pathway (the expression down-regulation of genes such as HSL, CPT-1 and AMPK-α, etc.). |
| first_indexed | 2024-03-10T23:16:09Z |
| format | Article |
| id | doaj.art-2d3bf3adbc294862ad20d8b13eaf2c3e |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T23:16:09Z |
| publishDate | 2023-08-01 |
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| series | Nutrients |
| spelling | doaj.art-2d3bf3adbc294862ad20d8b13eaf2c3e2023-11-19T08:38:25ZengMDPI AGNutrients2072-66432023-08-011517373610.3390/nu15173736Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese MiceChangbao Sun0Ang Li1Huan Wang2Jiage Ma3Juncai Hou4College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, ChinaCollege of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, ChinaBranch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaAcetate is associated with adipocyte differentiation and lipid deposition. To further develop this scientific point, obese mice on a high-fat diet were given an intragastric administration of acetate for 8 weeks and mouse adipose mesenchymal stem cells (mAMSCs) were treated with acetate for 24 h. The results showed that the body weight, food intake, Lee’s index, adipose tissue coefficient, liver index, blood lipid levels, insulin resistance, pro-inflammatory factors levels and fatty lesions in liver and adipose tissue in obese mice treated with acetate increased markedly, while anti-inflammatory factors levels and liver function decreased significantly (<i>p</i> < 0.05). The mRNA expression levels of PPAR-γ, C/EBP-α, SREBP, AFABP, FAS, ACC-1, SCD-1, LPL, LEPR, GPR41 and GPR43 genes in adipose tissue and mAMSCs were significantly increased, while the mRNA expression levels of HSL, CPT-1, CPT-2, AMPK, AdipoR1 and AdipoR2 genes were significantly reduced (<i>p</i> < 0.05). Except for AMPK-α signaling pathway proteins, the phosphorylation levels of p38 MAPK, ERK1/2, JNK and mTOR were significantly increased (<i>p</i> < 0.05) and these changes were dose-dependent. The findings indicated that acetate played a positive role in regulating adipocyte differentiation and lipid deposition by activating MAPKs and mTOR signaling pathways (the expression up-regulation of genes such as PPAR-γ, C/EBP-α and SREBP-1, etc.) and inhibiting the AMPK signaling pathway (the expression down-regulation of genes such as HSL, CPT-1 and AMPK-α, etc.).https://www.mdpi.com/2072-6643/15/17/3736obesitylipid metabolismacetateadipocyte differentiationlipid deposition |
| spellingShingle | Changbao Sun Ang Li Huan Wang Jiage Ma Juncai Hou Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice Nutrients obesity lipid metabolism acetate adipocyte differentiation lipid deposition |
| title | Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice |
| title_full | Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice |
| title_fullStr | Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice |
| title_full_unstemmed | Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice |
| title_short | Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice |
| title_sort | positive regulation of acetate in adipocyte differentiation and lipid deposition in obese mice |
| topic | obesity lipid metabolism acetate adipocyte differentiation lipid deposition |
| url | https://www.mdpi.com/2072-6643/15/17/3736 |
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