Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head
Background: Steroid-induced Osteonecrosis of the Femoral Head (SIONFH) is a skeletal disease with a high incidence and a poor prognosis. Whole body vibration therapy (WBVT), a new type of physical training, is known to promote bone formation. However, it remains unclear whether WBVT has a therapeuti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1251634/full |
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| author | Jia-Qing Tian Teng-Fei Wei Yu-Rou Wei Fang-Jun Xiao Xian-Shun He Kun Lin Shun Lu Xiao-Ming He Xiao-Ming He Wei He Wei He Qiu-Shi Wei Qiu-Shi Wei Xiao-Wei Xiang Min-Cong He Min-Cong He |
| author_facet | Jia-Qing Tian Teng-Fei Wei Yu-Rou Wei Fang-Jun Xiao Xian-Shun He Kun Lin Shun Lu Xiao-Ming He Xiao-Ming He Wei He Wei He Qiu-Shi Wei Qiu-Shi Wei Xiao-Wei Xiang Min-Cong He Min-Cong He |
| author_sort | Jia-Qing Tian |
| collection | DOAJ |
| description | Background: Steroid-induced Osteonecrosis of the Femoral Head (SIONFH) is a skeletal disease with a high incidence and a poor prognosis. Whole body vibration therapy (WBVT), a new type of physical training, is known to promote bone formation. However, it remains unclear whether WBVT has a therapeutic effect on SIONFH.Materials and methods: Thirty adult male and female Sprague-Dawley (SD) rats were selected and randomly assigned to three experimental groups: the control group, the model group, and the mechanical vibration group, respectively. SIONFH induction was achieved through the combined administration of lipopolysaccharides (LPS) and methylprednisolone sodium succinate for injection (MPS). The femoral head samples underwent hematoxylin and eosin (H&E) staining to visualize tissue structures. Structural parameters of the region of interest (ROI) were compared using Micro-CT analysis. Immunohistochemistry was employed to assess the expression levels of Piezo1, BMP2, RUNX2, HIF-1, VEGF, CD31, while immunofluorescence was used to examine CD31 and Emcn expression levels.Results: The H&E staining results revealed a notable improvement in the ratio of empty lacuna in various groups following WBVT intervention. Immunohistochemical analysis showed that the expression levels of Piezo1, BMP2, RUNX2, HIF-1, VEGF, and CD31 in the WBVT group exhibited significant differences when compared to the Model group (p < 0.05). Additionally, immunofluorescence analysis demonstrated statistically significant differences in CD31 and Emcn expression levels between the WBVT group and the Model group (p < 0.05).Conclusion: WBVT upregulates Piezo1 to promote osteogenic differentiation, potentially by enhancing the HIF-1α/VEGF axis and regulating H-vessel angiogenesis through the activation of the Piezo1 ion channel. This mechanism may lead to improved blood flow supply and enhanced osteogenic differentiation within the femoral head. |
| first_indexed | 2024-03-11T19:15:55Z |
| format | Article |
| id | doaj.art-2d3c77f1c03846ebb7c6e86a12c35e01 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-11T19:15:55Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-2d3c77f1c03846ebb7c6e86a12c35e012023-10-09T08:19:43ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-10-011110.3389/fcell.2023.12516341251634Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral headJia-Qing Tian0Teng-Fei Wei1Yu-Rou Wei2Fang-Jun Xiao3Xian-Shun He4Kun Lin5Shun Lu6Xiao-Ming He7Xiao-Ming He8Wei He9Wei He10Qiu-Shi Wei11Qiu-Shi Wei12Xiao-Wei Xiang13Min-Cong He14Min-Cong He15The Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaGuangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaGuangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, Guangdong, ChinaThe Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaGuangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, Guangdong, ChinaShenzhen Luohu Traditional Chinese Medicine Hospital, Shenzhen, Guangdong, ChinaThe Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaGuangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, Guangdong, ChinaBackground: Steroid-induced Osteonecrosis of the Femoral Head (SIONFH) is a skeletal disease with a high incidence and a poor prognosis. Whole body vibration therapy (WBVT), a new type of physical training, is known to promote bone formation. However, it remains unclear whether WBVT has a therapeutic effect on SIONFH.Materials and methods: Thirty adult male and female Sprague-Dawley (SD) rats were selected and randomly assigned to three experimental groups: the control group, the model group, and the mechanical vibration group, respectively. SIONFH induction was achieved through the combined administration of lipopolysaccharides (LPS) and methylprednisolone sodium succinate for injection (MPS). The femoral head samples underwent hematoxylin and eosin (H&E) staining to visualize tissue structures. Structural parameters of the region of interest (ROI) were compared using Micro-CT analysis. Immunohistochemistry was employed to assess the expression levels of Piezo1, BMP2, RUNX2, HIF-1, VEGF, CD31, while immunofluorescence was used to examine CD31 and Emcn expression levels.Results: The H&E staining results revealed a notable improvement in the ratio of empty lacuna in various groups following WBVT intervention. Immunohistochemical analysis showed that the expression levels of Piezo1, BMP2, RUNX2, HIF-1, VEGF, and CD31 in the WBVT group exhibited significant differences when compared to the Model group (p < 0.05). Additionally, immunofluorescence analysis demonstrated statistically significant differences in CD31 and Emcn expression levels between the WBVT group and the Model group (p < 0.05).Conclusion: WBVT upregulates Piezo1 to promote osteogenic differentiation, potentially by enhancing the HIF-1α/VEGF axis and regulating H-vessel angiogenesis through the activation of the Piezo1 ion channel. This mechanism may lead to improved blood flow supply and enhanced osteogenic differentiation within the femoral head.https://www.frontiersin.org/articles/10.3389/fcell.2023.1251634/fullsteroid-induced osteonecrosis of the femoral headwhole body vibration therapymechanical stimulationblood flow supplyosteogenic differentiation; H-vessels |
| spellingShingle | Jia-Qing Tian Teng-Fei Wei Yu-Rou Wei Fang-Jun Xiao Xian-Shun He Kun Lin Shun Lu Xiao-Ming He Xiao-Ming He Wei He Wei He Qiu-Shi Wei Qiu-Shi Wei Xiao-Wei Xiang Min-Cong He Min-Cong He Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head Frontiers in Cell and Developmental Biology steroid-induced osteonecrosis of the femoral head whole body vibration therapy mechanical stimulation blood flow supply osteogenic differentiation; H-vessels |
| title | Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head |
| title_full | Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head |
| title_fullStr | Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head |
| title_full_unstemmed | Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head |
| title_short | Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head |
| title_sort | effect of whole body vibration therapy in the rat model of steroid induced osteonecrosis of the femoral head |
| topic | steroid-induced osteonecrosis of the femoral head whole body vibration therapy mechanical stimulation blood flow supply osteogenic differentiation; H-vessels |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1251634/full |
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