Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood
Ultraviolet blood irradiation (UVBI) is an alternative approach to the treatment of infectious diseases of various pathogeneses. Recently, UVBI has attracted particular interest as a new immunomodulatory method. Experimental studies available in the literature demonstrate the absence of precise mech...
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MDPI AG
2023-06-01
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| Series: | Molecules |
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| author | Madina M. Sozarukova Nadezhda A. Skachko Polina A. Chilikina Dmitriy O. Novikov Elena V. Proskurnina |
| author_facet | Madina M. Sozarukova Nadezhda A. Skachko Polina A. Chilikina Dmitriy O. Novikov Elena V. Proskurnina |
| author_sort | Madina M. Sozarukova |
| collection | DOAJ |
| description | Ultraviolet blood irradiation (UVBI) is an alternative approach to the treatment of infectious diseases of various pathogeneses. Recently, UVBI has attracted particular interest as a new immunomodulatory method. Experimental studies available in the literature demonstrate the absence of precise mechanisms of the effect of ultraviolet radiation (UV) on blood. Here, we investigated the effect of UV radiation of line-spectrum mercury lamp (doses up to 500 mJ/cm<sup>2</sup>) traditionally used in UVBI on the major humoral blood components: albumin, globulins and uric acid. Preliminary data on the effect of various doses of UV radiation of full-spectrum flash xenon lamp (doses up to 136 mJ/cm<sup>2</sup>), a new promising source for UVBI, on the major blood plasma protein, albumin, are presented. The research methodology included spectrofluorimetric analysis of the oxidative modification of proteins and analysis of the antioxidant activity of humoral blood components by chemiluminometry. The effect of UV radiation on albumin caused its oxidative modification and, accordingly, an impairment of the transport properties of the protein. At the same time, UV-modified albumin and γ-globulins acquired pronounced antioxidant properties compared to native samples. Uric acid mixed with albumin did not protect the protein against UV-induced oxidation. The flash full-spectrum UV qualitatively had the same effect on albumin as line-spectrum UV did, but an order of magnitude lower doses were required to achieve comparable effects. The suggested protocol can be used for selecting a safe individual dose for UV therapy. |
| first_indexed | 2024-03-11T02:07:00Z |
| format | Article |
| id | doaj.art-2d3cafeabd5a4000a107b236ce50027d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T02:07:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-2d3cafeabd5a4000a107b236ce50027d2023-11-18T11:48:26ZengMDPI AGMolecules1420-30492023-06-012812464610.3390/molecules28124646Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human BloodMadina M. Sozarukova0Nadezhda A. Skachko1Polina A. Chilikina2Dmitriy O. Novikov3Elena V. Proskurnina4Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky av., 31, 119991 Moscow, RussiaDepartment of Plasma Power Plants, Bauman Moscow State Technical University, 2-nd Baumanskaya, 5, 105005 Moscow, RussiaDepartment of Plasma Power Plants, Bauman Moscow State Technical University, 2-nd Baumanskaya, 5, 105005 Moscow, RussiaDepartment of Plasma Power Plants, Bauman Moscow State Technical University, 2-nd Baumanskaya, 5, 105005 Moscow, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, 115522 Moscow, RussiaUltraviolet blood irradiation (UVBI) is an alternative approach to the treatment of infectious diseases of various pathogeneses. Recently, UVBI has attracted particular interest as a new immunomodulatory method. Experimental studies available in the literature demonstrate the absence of precise mechanisms of the effect of ultraviolet radiation (UV) on blood. Here, we investigated the effect of UV radiation of line-spectrum mercury lamp (doses up to 500 mJ/cm<sup>2</sup>) traditionally used in UVBI on the major humoral blood components: albumin, globulins and uric acid. Preliminary data on the effect of various doses of UV radiation of full-spectrum flash xenon lamp (doses up to 136 mJ/cm<sup>2</sup>), a new promising source for UVBI, on the major blood plasma protein, albumin, are presented. The research methodology included spectrofluorimetric analysis of the oxidative modification of proteins and analysis of the antioxidant activity of humoral blood components by chemiluminometry. The effect of UV radiation on albumin caused its oxidative modification and, accordingly, an impairment of the transport properties of the protein. At the same time, UV-modified albumin and γ-globulins acquired pronounced antioxidant properties compared to native samples. Uric acid mixed with albumin did not protect the protein against UV-induced oxidation. The flash full-spectrum UV qualitatively had the same effect on albumin as line-spectrum UV did, but an order of magnitude lower doses were required to achieve comparable effects. The suggested protocol can be used for selecting a safe individual dose for UV therapy.https://www.mdpi.com/1420-3049/28/12/4646ultravioletmercury lampflash xenon lampblood plasmahuman serum albuminuric acid |
| spellingShingle | Madina M. Sozarukova Nadezhda A. Skachko Polina A. Chilikina Dmitriy O. Novikov Elena V. Proskurnina Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood Molecules ultraviolet mercury lamp flash xenon lamp blood plasma human serum albumin uric acid |
| title | Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood |
| title_full | Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood |
| title_fullStr | Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood |
| title_full_unstemmed | Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood |
| title_short | Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood |
| title_sort | effect of low dose line spectrum and full spectrum uv on major humoral components of human blood |
| topic | ultraviolet mercury lamp flash xenon lamp blood plasma human serum albumin uric acid |
| url | https://www.mdpi.com/1420-3049/28/12/4646 |
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