A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples
Infectious disease outbreaks remain an ever‐prevalent global issue. The associated demand for rapid diagnostics and onsite testing will play an increasing and critical role in disease surveillance, prevention of the spread of infection, as well as timely commencement of treatment. Reported here is a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-06-01
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| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202100140 |
| _version_ | 1829459520827424768 |
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| author | Ffion Walters Gregory Burwell Jacob John Mitchell Muhammad Munem Ali Ehsaneh Daghigh Ahmadi A. Bernardus Mostert Cerys Anne Jenkins Sergiy Rozhko Olga Kazakova Owen J. Guy |
| author_facet | Ffion Walters Gregory Burwell Jacob John Mitchell Muhammad Munem Ali Ehsaneh Daghigh Ahmadi A. Bernardus Mostert Cerys Anne Jenkins Sergiy Rozhko Olga Kazakova Owen J. Guy |
| author_sort | Ffion Walters |
| collection | DOAJ |
| description | Infectious disease outbreaks remain an ever‐prevalent global issue. The associated demand for rapid diagnostics and onsite testing will play an increasing and critical role in disease surveillance, prevention of the spread of infection, as well as timely commencement of treatment. Reported here is a graphene–gold nanoparticle hybrid sensor platform technology that is demonstrated for the real‐time detection of viral proteins utilizing low volume samples (5 μL). Hepatitis C virus (HCV) is still an endemic problem worldwide and is used as an exemplar system here to demonstrate the capability of the platform viral detection sensor technology. Hepatitis C virus core antigen (HCVcAg) is a promising marker for point‐of‐care (POC) diagnostic testing for active HCV infection, with the potential to provide a one‐stop diagnosis and trigger for the commencement of treatment. Real‐time electrical resistance measurements are performed using various concentrations of HCVcAg with linear concentration dependence of resistance on HCVcAg concentration over the range of 100–750 pg mL−1. |
| first_indexed | 2024-12-12T11:19:22Z |
| format | Article |
| id | doaj.art-2d401b55c01b4776a9ccd3fc3977a988 |
| institution | Directory Open Access Journal |
| issn | 2699-9307 |
| language | English |
| last_indexed | 2024-12-12T11:19:22Z |
| publishDate | 2022-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj.art-2d401b55c01b4776a9ccd3fc3977a9882022-12-22T00:26:03ZengWiley-VCHAdvanced NanoBiomed Research2699-93072022-06-0126n/an/a10.1002/anbr.202100140A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume SamplesFfion Walters0Gregory Burwell1Jacob John Mitchell2Muhammad Munem Ali3Ehsaneh Daghigh Ahmadi4A. Bernardus Mostert5Cerys Anne Jenkins6Sergiy Rozhko7Olga Kazakova8Owen J. Guy9Centre for NanoHealth, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKDepartment of Physics, School of Biosciences, Geography and Physics Swansea University Swansea SA2 8PP UKCentre for NanoHealth, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKCentre for NanoHealth, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKCentre for NanoHealth, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKDepartment of Chemistry, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKSwansea University Medical School Swansea University Swansea SA2 8PP UKNational Physical Laboratory Quantum Metrology Institute Teddington Middlesex TW11 0LW UKNational Physical Laboratory Quantum Metrology Institute Teddington Middlesex TW11 0LW UKCentre for NanoHealth, School of Engineering and Applied Sciences Swansea University Swansea SA2 8PP UKInfectious disease outbreaks remain an ever‐prevalent global issue. The associated demand for rapid diagnostics and onsite testing will play an increasing and critical role in disease surveillance, prevention of the spread of infection, as well as timely commencement of treatment. Reported here is a graphene–gold nanoparticle hybrid sensor platform technology that is demonstrated for the real‐time detection of viral proteins utilizing low volume samples (5 μL). Hepatitis C virus (HCV) is still an endemic problem worldwide and is used as an exemplar system here to demonstrate the capability of the platform viral detection sensor technology. Hepatitis C virus core antigen (HCVcAg) is a promising marker for point‐of‐care (POC) diagnostic testing for active HCV infection, with the potential to provide a one‐stop diagnosis and trigger for the commencement of treatment. Real‐time electrical resistance measurements are performed using various concentrations of HCVcAg with linear concentration dependence of resistance on HCVcAg concentration over the range of 100–750 pg mL−1.https://doi.org/10.1002/anbr.202100140chemiresistorgold nanoparticlesgraphene biosensorhepatitis C virus core antigen (HCVcAg)hybridreal-time |
| spellingShingle | Ffion Walters Gregory Burwell Jacob John Mitchell Muhammad Munem Ali Ehsaneh Daghigh Ahmadi A. Bernardus Mostert Cerys Anne Jenkins Sergiy Rozhko Olga Kazakova Owen J. Guy A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples Advanced NanoBiomed Research chemiresistor gold nanoparticles graphene biosensor hepatitis C virus core antigen (HCVcAg) hybrid real-time |
| title | A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples |
| title_full | A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples |
| title_fullStr | A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples |
| title_full_unstemmed | A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples |
| title_short | A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples |
| title_sort | rapid graphene sensor platform for the detection of viral proteins in low volume samples |
| topic | chemiresistor gold nanoparticles graphene biosensor hepatitis C virus core antigen (HCVcAg) hybrid real-time |
| url | https://doi.org/10.1002/anbr.202100140 |
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