Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity
Abstract Background The injection of rabies immune globulin (RIG) is of the utmost importance in the management of category III exposures to rabies-suspect animals. Because of the high cost and limited availability of existing RIG, one possible replacement for RIG is monoclonal antibodies (MAbs) aga...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | Infectious Diseases of Poverty |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40249-018-0500-x |
| _version_ | 1811269669221302272 |
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| author | Peng-Cheng Yu Xiao-Yan Tao Li-Hua Wang Qing Tang Li-Yun Fan Shu-Xia Zhang Shu-Qing Liu Xue-Xin Lu Gui-Zhen Wu Wu-Yang Zhu |
| author_facet | Peng-Cheng Yu Xiao-Yan Tao Li-Hua Wang Qing Tang Li-Yun Fan Shu-Xia Zhang Shu-Qing Liu Xue-Xin Lu Gui-Zhen Wu Wu-Yang Zhu |
| author_sort | Peng-Cheng Yu |
| collection | DOAJ |
| description | Abstract Background The injection of rabies immune globulin (RIG) is of the utmost importance in the management of category III exposures to rabies-suspect animals. Because of the high cost and limited availability of existing RIG, one possible replacement for RIG is monoclonal antibodies (MAbs) against the rabies virus (RABV). Consequently, it is necessary to determine the neutralizing activity of the MAbs against rabies viruses, especially street rabies virus. However, the method to detect the neutralizing activity of MAbs against street rabies virus remains undefined. Methods To establish a method for detecting the neutralizing activity of MAbs against street rabies virus, we constructed a library consisting of 12 strains of street RABV from 11 provinces in China. Using this street RABV library and the Reed–Muench formula, we established a method for detecting the neutralizing titer of the MAbs. The reliability and repeatability of the method were evaluated by repeatedly measuring the neutralizing activity of a MAb and a post vaccination serum. Results A total of 12 strains of street RABV were chosen for inclusion in the street RABV library, which covered six Chinese lineages (China I–China VI) and grew to high titers in N2A cells (> 105 FFD50/ml). On the basis of the library, we constructed the method to detect the neutralizing activity of the MAbs. The results of repeatedly measuring the MAbs and positive serum showed excellent reliability and repeatability of the method established in this study. Conclusions This study established a street RABV library reflecting the epidemiological features of Chinese rabies viruses, which provides a platform for detecting the neutralizing activity of MAbs against rabies viruses circulating in China. |
| first_indexed | 2024-04-12T21:46:21Z |
| format | Article |
| id | doaj.art-2d41372b2cdc444086e784d472aa8959 |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-04-12T21:46:21Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-2d41372b2cdc444086e784d472aa89592022-12-22T03:15:37ZengBMCInfectious Diseases of Poverty2049-99572018-12-01711710.1186/s40249-018-0500-xEstablishment of a Chinese street rabies virus library and its application for detecting neutralizing activityPeng-Cheng Yu0Xiao-Yan Tao1Li-Hua Wang2Qing Tang3Li-Yun Fan4Shu-Xia Zhang5Shu-Qing Liu6Xue-Xin Lu7Gui-Zhen Wu8Wu-Yang Zhu9Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionKey Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionAbstract Background The injection of rabies immune globulin (RIG) is of the utmost importance in the management of category III exposures to rabies-suspect animals. Because of the high cost and limited availability of existing RIG, one possible replacement for RIG is monoclonal antibodies (MAbs) against the rabies virus (RABV). Consequently, it is necessary to determine the neutralizing activity of the MAbs against rabies viruses, especially street rabies virus. However, the method to detect the neutralizing activity of MAbs against street rabies virus remains undefined. Methods To establish a method for detecting the neutralizing activity of MAbs against street rabies virus, we constructed a library consisting of 12 strains of street RABV from 11 provinces in China. Using this street RABV library and the Reed–Muench formula, we established a method for detecting the neutralizing titer of the MAbs. The reliability and repeatability of the method were evaluated by repeatedly measuring the neutralizing activity of a MAb and a post vaccination serum. Results A total of 12 strains of street RABV were chosen for inclusion in the street RABV library, which covered six Chinese lineages (China I–China VI) and grew to high titers in N2A cells (> 105 FFD50/ml). On the basis of the library, we constructed the method to detect the neutralizing activity of the MAbs. The results of repeatedly measuring the MAbs and positive serum showed excellent reliability and repeatability of the method established in this study. Conclusions This study established a street RABV library reflecting the epidemiological features of Chinese rabies viruses, which provides a platform for detecting the neutralizing activity of MAbs against rabies viruses circulating in China.http://link.springer.com/article/10.1186/s40249-018-0500-xStreet RABVMAbsNeutralizing activity |
| spellingShingle | Peng-Cheng Yu Xiao-Yan Tao Li-Hua Wang Qing Tang Li-Yun Fan Shu-Xia Zhang Shu-Qing Liu Xue-Xin Lu Gui-Zhen Wu Wu-Yang Zhu Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity Infectious Diseases of Poverty Street RABV MAbs Neutralizing activity |
| title | Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity |
| title_full | Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity |
| title_fullStr | Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity |
| title_full_unstemmed | Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity |
| title_short | Establishment of a Chinese street rabies virus library and its application for detecting neutralizing activity |
| title_sort | establishment of a chinese street rabies virus library and its application for detecting neutralizing activity |
| topic | Street RABV MAbs Neutralizing activity |
| url | http://link.springer.com/article/10.1186/s40249-018-0500-x |
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