TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11493 |
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| author | Feilong Guo Tianci Wu Gangbiao Xu Haijun Qi Xiuliang Zhu Zengyan Zhang |
| author_facet | Feilong Guo Tianci Wu Gangbiao Xu Haijun Qi Xiuliang Zhu Zengyan Zhang |
| author_sort | Feilong Guo |
| collection | DOAJ |
| description | Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis</i>. The roles of the wheat-wall-associated kinases (WAKs) in defense against both <i>F. graminearum</i> and <i>R. cerealis</i> have remained largely unknown. This research reports the identification of <i>TaWAK2A-800</i>, a wheat <i>WAK</i>-coding gene located on chromosome 2A, and its functional roles in wheat resistance responses to FHB and sharp eyespot. <i>TaWAK2A-800</i> transcript abundance was elevated by the early infection of <i>R. cerealis</i> and <i>F. graminearum</i>, or treatment with exogenous chitin. The gene transcript seemed to correspond to the resistance of wheat. Further functional analyses showed that silencing <i>TaWAK2A-800</i> compromised the resistance of wheat to both FHB (<i>F. graminearum</i>) and sharp eyespot (<i>R. cerealis</i>). Moreover, the silencing reduced the expression levels of six defense-related genes, including the chitin-triggering immune pathway-marker genes, <i>TaCERK1</i>, <i>TaRLCK1B</i>, and <i>TaMPK3</i>. Summarily, <i>TaWAK2A-800</i> participates positively in the resistance responses to both <i>F. graminearum</i> and <i>R. cerealis</i>, possibly through a chitin-induced pathway in wheat. <i>TaWAK2A-800</i> will be useful for breeding wheat varieties with resistance to both FHB and sharp eyespot. |
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| language | English |
| last_indexed | 2024-03-10T06:01:26Z |
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| spelling | doaj.art-2d42f2e9b07b44c3ab9050878670dade2023-11-22T20:53:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211149310.3390/ijms222111493TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in WheatFeilong Guo0Tianci Wu1Gangbiao Xu2Haijun Qi3Xiuliang Zhu4Zengyan Zhang5The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe Laboratory of Forestry Genetics, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaFusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis</i>. The roles of the wheat-wall-associated kinases (WAKs) in defense against both <i>F. graminearum</i> and <i>R. cerealis</i> have remained largely unknown. This research reports the identification of <i>TaWAK2A-800</i>, a wheat <i>WAK</i>-coding gene located on chromosome 2A, and its functional roles in wheat resistance responses to FHB and sharp eyespot. <i>TaWAK2A-800</i> transcript abundance was elevated by the early infection of <i>R. cerealis</i> and <i>F. graminearum</i>, or treatment with exogenous chitin. The gene transcript seemed to correspond to the resistance of wheat. Further functional analyses showed that silencing <i>TaWAK2A-800</i> compromised the resistance of wheat to both FHB (<i>F. graminearum</i>) and sharp eyespot (<i>R. cerealis</i>). Moreover, the silencing reduced the expression levels of six defense-related genes, including the chitin-triggering immune pathway-marker genes, <i>TaCERK1</i>, <i>TaRLCK1B</i>, and <i>TaMPK3</i>. Summarily, <i>TaWAK2A-800</i> participates positively in the resistance responses to both <i>F. graminearum</i> and <i>R. cerealis</i>, possibly through a chitin-induced pathway in wheat. <i>TaWAK2A-800</i> will be useful for breeding wheat varieties with resistance to both FHB and sharp eyespot.https://www.mdpi.com/1422-0067/22/21/11493bread wheat (<i>Triticum aestivum</i>)chitin-induced immunity<i>Fusarium graminearum</i><i>Rhizoctonia cereal</i>wall-associated kinase (WAK) |
| spellingShingle | Feilong Guo Tianci Wu Gangbiao Xu Haijun Qi Xiuliang Zhu Zengyan Zhang TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat International Journal of Molecular Sciences bread wheat (<i>Triticum aestivum</i>) chitin-induced immunity <i>Fusarium graminearum</i> <i>Rhizoctonia cereal</i> wall-associated kinase (WAK) |
| title | TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat |
| title_full | TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat |
| title_fullStr | TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat |
| title_full_unstemmed | TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat |
| title_short | TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat |
| title_sort | tawak2a 800 a wall associated kinase participates positively in resistance to fusarium head blight and sharp eyespot in wheat |
| topic | bread wheat (<i>Triticum aestivum</i>) chitin-induced immunity <i>Fusarium graminearum</i> <i>Rhizoctonia cereal</i> wall-associated kinase (WAK) |
| url | https://www.mdpi.com/1422-0067/22/21/11493 |
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